Recombinant Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)
![Western blot - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)](https://www.abcam.com/ps/products/247/ab247712/Images/ab247712-478766-anti-glycophorin-c-gpc-antibody-epr4116-westernblot-human.jpg "Western blot - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4116] to Glycophorin C/GPC - BSA and Azide free
- Suitable for: IHC-P, IP, Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free
See all Glycophorin C/GPC primary antibodies -
Description
Rabbit monoclonal [EPR4116] to Glycophorin C/GPC - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: K562 cell lysate; IP: TF-1 lysate.
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General notes
ab247712 is the carrier-free version of ab108925.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR4116 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247712 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| IP |
Use at an assay dependent concentration.
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
IP
Use at an assay dependent concentration. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. |
Target
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Function
This protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells. -
Tissue specificity
Glycophorin-C is expressed in erythrocytes. Glycophorin-D is ubiquitous. -
Sequence similarities
Belongs to the glycophorin-C family. -
Cellular localization
Cell membrane. Linked to the membrane via band 4.1. - Information by UniProt
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Database links
- Entrez Gene: 2995 Human
- Omim: 110750 Human
- SwissProt: P04921 Human
- Unigene: 59138 Human
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Alternative names
- CD236 antibody
- CD236R antibody
- GE antibody
see all
Images
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Western blot - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)All lanes : Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925) at 1/1000 dilution (Purified)
Lane 1 : TF-1 (Human Erythroleukemia erythroblast) whole cell lysate
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 : Human red blood lysate
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 14 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)](https://www.abcam.com/ps/products/247/ab247712/Images/ab247712-14-anti-glycophorin-c-gpc-antibody-epr4116-immunohistochemistry-spleen-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)This data was developed using ab108925, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling Glycophorin C/GPC with Purified ab108925 at 1:1500 dilution (0.06 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)](https://www.abcam.com/ps/products/247/ab247712/Images/ab247712-13-anti-glycophorin-c-gpc-antibody-epr4116-immunohistochemistry-placenta-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)This data was developed using ab108925, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue sections labeling Glycophorin C/GPC with Purified ab108925 at 1:1500 dilution (0.06 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. M3 -
![Immunoprecipitation - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)](https://www.abcam.com/ps/products/247/ab247712/Images/ab247712-10-anti-glycophorin-c-gpc-antibody-epr4116-immunoprecipitation-tf1-human.jpg)
Immunoprecipitation - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)This data was developed using ab108925, the same antibody clone in a different buffer formulation.
Glycophorin C/GPC was immunoprecipitated from 0.35 mg TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10 µg with ab108925 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.Lane 1: TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10 µg
Lane 2: abab108925 IP in TF-1 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab108925 in TF-1 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Lower band could be N-Deglycosylated GPC. (PMID: 16461900)
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![Flow Cytometry (Intracellular) - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)](https://www.abcam.com/ps/products/247/ab247712/Images/ab247712-9-anti-glycophorin-c-gpc-antibody-epr4116-flowcytometry-a549-k562-human.jpg)
Flow Cytometry (Intracellular) - Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)This data was developed using ab108925, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell, Left) / K-562 (Human chronic myelogenous leukemia lymphoblast, Right) cells labeling Glycophorin C/GPC with purified ab108925 at 1/10000 dilution (0.1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Negative control: A549. -
![Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)](https://www.abcam.com/ps/products/247/ab247712/Images/ab247712-6-benefits-of-recombinant-antibodies.png)
Anti-Glycophorin C/GPC antibody [EPR4116] - BSA and Azide free (ab247712)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab247712 has not yet been referenced specifically in any publications.
