Recombinant Anti-Glypican 3 antibody [EPR10641] (ab174851)
![Western blot - Anti-Glypican 3 antibody [EPR10641] (ab174851)](https://www.abcam.com/ps/products/174/ab174851/Images/ab174851-298738-anti-glypican-3-antibody-epr10641-western-blot.jpg "Western blot - Anti-Glypican 3 antibody [EPR10641] (ab174851)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10641] to Glypican 3
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Glypican 3 antibody [EPR10641]
See all Glypican 3 primary antibodies -
Description
Rabbit monoclonal [EPR10641] to Glypican 3 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment within Human Glypican 3. The exact sequence is proprietary.
Database link: P51654 -
Positive control
- HepG2 whole cell lysate (ab7900); Human fetal liver lysate; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10641 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 66 kDa.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/5000. Predicted molecular weight: 66 kDa. |
Target
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Function
Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. -
Tissue specificity
Highly expressed in lung, liver and kidney. -
Involvement in disease
Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. -
Sequence similarities
Belongs to the glypican family. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 2719 Human
- Omim: 300037 Human
- SwissProt: P51654 Human
- Unigene: 644108 Human
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Alternative names
- DGSX antibody
- Glypican proteoglycan 3 antibody
- Glypican-3 [Precursor] antibody
see all
Images
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Western blot - Anti-Glypican 3 antibody [EPR10641] (ab174851)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: GPC3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab174851 observed at 70 kDa. Red - loading control, ab130007, observed at 125 kDa.
ab174851 was shown to recognize Glypican 3 in wild-type HAP1 cells as signal was lost at the expected MW in GPC3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GPC3 knockout samples were subjected to SDS-PAGE. Ab174851 and ab130007 (Mouse anti-vinculin loading control) were incubated overnight at 4°C at 1/1000 and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry (Intracellular) - Anti-Glypican 3 antibody [EPR10641] (ab174851)](https://www.abcam.com/ps/products/174/ab174851/Images/ab174851-202329-anti-glypican-3-antibody-epr10641-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Glypican 3 antibody [EPR10641] (ab174851)Intracellular flow cytometric analysis of permeabilized HepG2 cells labeling Glypican 3 using ab174851 at 1/10 dilution (red) or a rabbit IgG negative (green).
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![Western blot - Anti-Glypican 3 antibody [EPR10641] (ab174851)](https://www.abcam.com/ps/products/174/ab174851/Images/ab174851-202328-anti-glypican-3-antibody-epr10641-western-blot.jpg)
Western blot - Anti-Glypican 3 antibody [EPR10641] (ab174851)All lanes : Anti-Glypican 3 antibody [EPR10641] (ab174851) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 66 kDa -
![Anti-Glypican 3 antibody [EPR10641] (ab174851)](https://www.abcam.com/ps/products/174/ab174851/Images/ab174851-4-benefits-of-recombinant-antibodies.png)
Anti-Glypican 3 antibody [EPR10641] (ab174851)
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab174851 has been referenced in 1 publication.
- Wu F et al. Screening of MicroRNA Related to Irradiation Response and the Regulation Mechanism of miRNA-96-5p in Rectal Cancer Cells. Front Oncol 11:699475 (2021). PubMed: 34458143