Recombinant Anti-Glypican 3 antibody [EPR20569] (ab207080)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] (ab207080)](https://www.abcam.com/ps/products/207/ab207080/Images/ab207080-280339-anti-glypican-3-antibody-epr20569-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] (ab207080)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20569] to Glypican 3
- Suitable for: WB, Flow Cyt (Intra), IHC-P, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Glypican 3 antibody [EPR20569]
See all Glypican 3 primary antibodies -
Description
Rabbit monoclonal [EPR20569] to Glypican 3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra), IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HepG2 and Huh7 whole cell lysates; Human fetal liver lysate. IHC-P: Human placenta and hepatocellular cancer tissues. ICC/IF: HepG2 cells. Flow Cyt (intra): HepG2 cells. IP: HepG2 whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20569 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab207080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 40,70 kDa (predicted molecular weight: 65 kDa).
|
|
| Flow Cyt (Intra) |
1/500.
|
|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/500.
|
|
| IP |
1/40.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 40,70 kDa (predicted molecular weight: 65 kDa). |
|
Flow Cyt (Intra)
1/500. |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/500. |
|
IP
1/40. |
Target
-
Function
Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. -
Tissue specificity
Highly expressed in lung, liver and kidney. -
Involvement in disease
Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. -
Sequence similarities
Belongs to the glypican family. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
-
Database links
- Entrez Gene: 2719 Human
- Omim: 300037 Human
- SwissProt: P51654 Human
- Unigene: 644108 Human
-
Alternative names
- DGSX antibody
- Glypican proteoglycan 3 antibody
- Glypican-3 [Precursor] antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] (ab207080)
Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Glypican 3 with ab207080 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human placenta (PMID: 12788060; PMID: 11193214). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-Glypican 3 antibody [EPR20569] (ab207080)](https://www.abcam.com/ps/products/207/ab207080/Images/ab207080-280340-anti-glypican-3-antibody-epr20569-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Glypican 3 antibody [EPR20569] (ab207080)Immunofluorescent analysis of 100% methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Glypican 3 with ab207080 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on HepG2 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Negative control: Raji (PMID: 9371521).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] (ab207080)](https://www.abcam.com/ps/products/207/ab207080/Images/ab207080-280337-anti-glypican-3-antibody-epr20569-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] (ab207080)Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer tissue labeling Glypican 3 with ab207080 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human hepatocellular cancer (PMID: 12788060). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunoprecipitation - Anti-Glypican 3 antibody [EPR20569] (ab207080)](https://www.abcam.com/ps/products/207/ab207080/Images/ab207080-9-anti-glypican-3-antibody-epr20569-immunoprecipitation-hepg2-human.jpg)
Immunoprecipitation - Anti-Glypican 3 antibody [EPR20569] (ab207080)Purified ab207080 at 1/40 dilution (2µg) immunoprecipitating Glypican 3 in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate 10 µg
Lane 2 (+): ab207080 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab207080 in HepG2 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/10,000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 40,70 kDa -
![Flow Cytometry (Intracellular) - Anti-Glypican 3 antibody [EPR20569] (ab207080)](https://www.abcam.com/ps/products/207/ab207080/Images/ab207080-280341-anti-glypican-3-antibody-epr20569-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Glypican 3 antibody [EPR20569] (ab207080)Left panel - Negative control: Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Raji (human Burkitt's lymphoma) cell line labeling Glypican 3with ab207080 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
Right panel: Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling Glypican 3with ab207080 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] (ab207080)](https://www.abcam.com/ps/products/207/ab207080/Images/ab207080-280332-anti-glypican-3-antibody-epr20569-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] (ab207080)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Glypican 3 with ab207080 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Negative control: No staining on human liver (PMID: 12788060).
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Western blot - Anti-Glypican 3 antibody [EPR20569] (ab207080)](https://www.abcam.com/ps/products/207/ab207080/Images/ab207080-280329-anti-glypican-3-antibody-epr20569-western-blot.jpg)
Western blot - Anti-Glypican 3 antibody [EPR20569] (ab207080)All lanes : Anti-Glypican 3 antibody [EPR20569] (ab207080) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 65 kDa
Observed band size: 40,70 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
The observed molecular weight is consistent with the literature (PMID: 12851874; PMID: 25553423).
-
![Western blot - Anti-Glypican 3 antibody [EPR20569] (ab207080)](https://www.abcam.com/ps/products/207/ab207080/Images/ab207080-280328-anti-glypican-3-antibody-epr20569-western-blot.jpg)
Western blot - Anti-Glypican 3 antibody [EPR20569] (ab207080)All lanes : Anti-Glypican 3 antibody [EPR20569] (ab207080) at 1/1000 dilution
Lane 1 : Huh7 (human hepatocellular carcinoma epithelial cell line) whole cell lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 65 kDa
Observed band size: 40,70 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 minutes; Lane 2: 1 minute.
The observed molecular weight is consistent with the literature (PMID: 12851874; PMID: 25553423).
-
![Anti-Glypican 3 antibody [EPR20569] (ab207080)](https://www.abcam.com/ps/products/207/ab207080/Images/ab207080-8-benefits-of-recombinant-antibodies.png)
Anti-Glypican 3 antibody [EPR20569] (ab207080)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (2)
ab207080 has been referenced in 2 publications.
- Yang H et al. Therapeutic potential of targeting membrane-spanning proteoglycan SDC4 in hepatocellular carcinoma. Cell Death Dis 12:492 (2021). PubMed: 33990545
- Chu X et al. Establishment and characterization of a novel cell line (cc-006cpm8) of moderately/poorly differentiated colorectal adenocarcinoma derived from a primary tumor of a patient. Int J Oncol 55:243-256 (2019). PubMed: 31115570