Recombinant Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)](https://www.abcam.com/ps/products/240/ab240995/Images/ab240995-3-ab207080280339207080IHC1.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20569] to Glypican 3 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free
See all Glypican 3 primary antibodies -
Description
Rabbit monoclonal [EPR20569] to Glypican 3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human placenta and hepatocellular cancer tissues. ICC/IF: HepG2 cells. Flow Cyt (intra): HepG2 cells. IP: HepG2 whole cell lysate.
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General notes
ab240995 is the carrier-free version of ab207080.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20569 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 40,70 kDa (predicted molecular weight: 65 kDa).
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| ICC/IF |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 40,70 kDa (predicted molecular weight: 65 kDa). |
|
ICC/IF
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. -
Tissue specificity
Highly expressed in lung, liver and kidney. -
Involvement in disease
Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. -
Sequence similarities
Belongs to the glypican family. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 2719 Human
- Omim: 300037 Human
- SwissProt: P51654 Human
- Unigene: 644108 Human
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Alternative names
- DGSX antibody
- Glypican proteoglycan 3 antibody
- Glypican-3 [Precursor] antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)
Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Glypican 3 with ab207080 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human placenta (PMID: 12788060; PMID: 11193214). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207080)
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)](https://www.abcam.com/ps/products/240/ab240995/Images/ab240995-2-ab207080280340207080ICC1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)Immunofluorescent analysis of 100% methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Glypican 3 with ab207080 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on HepG2 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Negative control: Raji (PMID: 9371521).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207080) -
![Flow Cytometry (Intracellular) - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)](https://www.abcam.com/ps/products/240/ab240995/Images/ab240995-1-ab207080280341207080FCfinal.jpg)
Flow Cytometry (Intracellular) - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)Left panel - Negative control: Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Raji (human Burkitt's lymphoma) cell line labeling Glypican 3 with ab207080 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
Right panel: Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling Glypican 3 with ab207080 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207080)
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![Immunoprecipitation - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)](https://www.abcam.com/ps/products/240/ab240995/Images/ab240995-7-anti-glypican-3-antibody-epr20569-immunoprecipitation-hepg2-human.jpg)
Immunoprecipitation - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)This data was developed using ab207080, the same antibody clone in a different buffer formulation.
Purified ab207080 at 1/40 dilution (2µg) immunoprecipitating Glypican 3 in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate 10 µg
Lane 2 (+): ab207080 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab207080 in HepG2 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/10,000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 40,70 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)](https://www.abcam.com/ps/products/240/ab240995/Images/ab240995-4-ab207080280337207080IHC2final.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer tissue labeling Glypican 3 with ab207080 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human hepatocellular cancer (PMID: 12788060). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207080)
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)](https://www.abcam.com/ps/products/240/ab240995/Images/ab240995-5-ab207080280332207080IHCneg.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Glypican 3 with ab207080 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Negative control: No staining on human liver (PMID: 12788060).
Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207080)
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)](https://www.abcam.com/ps/products/240/ab240995/Images/ab240995-6-benefits-of-recombinant-antibodies.png)
Anti-Glypican 3 antibody [EPR20569] - BSA and Azide free (ab240995)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab240995 has not yet been referenced specifically in any publications.
