Recombinant Anti-GLYR1 antibody [EPR10076(B)] (ab154838)
![Western blot - Anti-GLYR1 antibody [EPR10076(B)] (ab154838)](https://www.abcam.com/ps/products/154/ab154838/Images/ab154838-410236-anti-glyr1-antibody-epr10076-western-blot-human-knockout-lysate.jpg "Western blot - Anti-GLYR1 antibody [EPR10076(B)] (ab154838)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10076(B)] to GLYR1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GLYR1 antibody [EPR10076(B)]
See all GLYR1 primary antibodies -
Description
Rabbit monoclonal [EPR10076(B)] to GLYR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T, Jurkat, HeLa, K562 and HUVEC cell lysates. IHC-P: Human colon and skin tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10076(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 61 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May have oxidoreductase activity. Regulates p38 MAP kinase activity by mediating stress activation of p38alpha/MAPK14 and specifically regulating MAPK14 signaling. Indirectly promotes phosphorylation of MAPK14 and activation of ATF2. The phosphorylation of MAPK14 requires upstream activity of MAP2K4 and MAP2K6. Recruited on chromatin, recognizes and binds trimethylated 'Lys-36' of histone H3 (H3K36me3). -
Sequence similarities
Belongs to the 3-hydroxyisobutyrate dehydrogenase family. NP60 subfamily.
Contains 1 A.T hook DNA-binding domain.
Contains 1 PWWP domain. -
Domain
The A.T hook DNA-binding domain is required for the interaction with MAPK14.
The PWWP domain probably mediates the binding to H3K36me3. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 84656 Human
- Entrez Gene: 74022 Mouse
- Entrez Gene: 360477 Rat
- Omim: 610660 Human
- SwissProt: Q49A26 Human
- SwissProt: Q922P9 Mouse
- SwissProt: Q5RKH0 Rat
- Unigene: 387255 Human
see all -
Alternative names
- 3 hydroxyisobutyrate dehydrogenase like protein antibody
- 3-hydroxyisobutyrate dehydrogenase-like protein antibody
- BM045 antibody
see all
Images
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Western blot - Anti-GLYR1 antibody [EPR10076(B)] (ab154838)All lanes : Anti-GLYR1 antibody [EPR10076(B)] (ab154838) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : GLYR1 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HUVEC cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDaLanes 1-4: Merged signal (red and green). Green - ab154838 observed at 61 kDa. Red - loading control ab8245 observed at 36 kDa.
ab154838 Anti-GLYR1 antibody [EPR10076(B)] was shown to specifically react with GLYR1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266610 (knockout cell lysate ab257968) was used. Wild-type and GLYR1 knockout samples were subjected to SDS-PAGE. ab154838 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-GLYR1 antibody [EPR10076(B)] (ab154838)](https://www.abcam.com/ps/products/154/ab154838/Images/GLYR1-Primary-antibodies-ab154838-1.jpg)
Western blot - Anti-GLYR1 antibody [EPR10076(B)] (ab154838)All lanes : Anti-GLYR1 antibody [EPR10076(B)] (ab154838) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYR1 antibody [EPR10076(B)] (ab154838)](https://www.abcam.com/ps/products/154/ab154838/Images/GLYR1-Primary-antibodies-ab154838-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYR1 antibody [EPR10076(B)] (ab154838)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue, labeling GLYR1 using ab154838 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYR1 antibody [EPR10076(B)] (ab154838)](https://www.abcam.com/ps/products/154/ab154838/Images/GLYR1-Primary-antibodies-ab154838-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYR1 antibody [EPR10076(B)] (ab154838)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue, labeling GLYR1 using ab154838 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-GLYR1 antibody [EPR10076(B)] (ab154838)](https://www.abcam.com/ps/products/154/ab154838/Images/ab154838-5-benefits-of-recombinant-antibodies.png)
Anti-GLYR1 antibody [EPR10076(B)] (ab154838)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154838 has not yet been referenced specifically in any publications.