Recombinant Anti-GNB2 antibody [EP3262Y] (ab108504)
![Western blot - Anti-GNB2 antibody [EP3262Y] (ab108504)](https://www.abcam.com/ps/products/108/ab108504/Images/ab108504-519658-AntiGNB2-antibody-EP3262Y-western-blot-human-mouse-rat.jpg "Western blot - Anti-GNB2 antibody [EP3262Y] (ab108504)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3262Y] to GNB2
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GNB2 antibody [EP3262Y]
See all GNB2 primary antibodies -
Description
Rabbit monoclonal [EP3262Y] to GNB2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, SH-SY5Y, Jurkat and NIH3T3 cell lysates. Rat and Mouse brain lysate
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General notes
This product has switched from a hybridoma to recombinant production method on 18th September 2023
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP3262Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).
For the unpurified version use at 1/1000 to 1/10000 dilution |
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa). For the unpurified version use at 1/1000 to 1/10000 dilution |
Target
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Function
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. -
Sequence similarities
Belongs to the WD repeat G protein beta family.
Contains 7 WD repeats. -
Cellular localization
Cytoplasm > perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 2783 Human
- Entrez Gene: 14693 Mouse
- Entrez Gene: 81667 Rat
- Omim: 139390 Human
- SwissProt: P62879 Human
- SwissProt: P62880 Mouse
- SwissProt: P54313 Rat
- Unigene: 185172 Human
see all -
Alternative names
- G protein beta 2 subunit antibody
- G protein subunit beta 2 antibody
- G protein subunit beta-2 antibody
see all
Images
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Western blot - Anti-GNB2 antibody [EP3262Y] (ab108504)All lanes : Anti-GNB2 antibody [EP3262Y] (ab108504) at 1/1000 dilution
Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 15 µg
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4 : Mouse brain lysate at 20 µg
Lane 5 : Rat brain lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Secondary antibody: incubated for 1h at 1:20,000 at room temperature (ab97051); incubated for 1h at 1:2000 at room temperature (Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG)
Exposure time: Lane 1, 5 seconds. Lanes 2 and 3, 10 seconds. Lanes 4 and 5, 3 seconds.
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![Western blot - Anti-GNB2 antibody [EP3262Y] (ab108504)](https://www.abcam.com/ps/products/108/ab108504/Images/ab108504-393577-anti-gnb2-antibody-ep3262y-western-blot-human-knockout-lysate.jpg)
Western blot - Anti-GNB2 antibody [EP3262Y] (ab108504)All lanes : Anti-GNB2 antibody [EP3262Y] (ab108504) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : GNB2 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab108504 observed at 35 kDa. Red - loading control ab7291 observed at 50 kDa.
ab108504 Anti-GNB2 antibody [EP3262Y] was shown to specifically react with GNB2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266528 (knockout cell lysate ab257453) was used. Wild-type and GNB2 knockout samples were subjected to SDS-PAGE. ab108504 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-GNB2 antibody [EP3262Y] (ab108504)](https://www.abcam.com/ps/products/108/ab108504/Images/ab108504-3-benefits-of-recombinant-antibodies.png)
Anti-GNB2 antibody [EP3262Y] (ab108504)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab108504 has been referenced in 1 publication.
- Abd Alla J et al. Stearoyl-CoA Desaturase (SCD) Induces Cardiac Dysfunction with Cardiac Lipid Overload and Angiotensin II AT1 Receptor Protein Up-Regulation. Int J Mol Sci 22:N/A (2021). PubMed: 34576047