Anti-GOLPH3/MIDAS antibody (ab91492)
Key features and details
- Rabbit polyclonal to GOLPH3/MIDAS
- Suitable for: ICC, WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-GOLPH3/MIDAS antibody
See all GOLPH3/MIDAS primary antibodies -
Description
Rabbit polyclonal to GOLPH3/MIDAS -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide corresponding to Human GOLPH3/MIDAS (N terminal).
Database link: AAH12123 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa.
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| Notes |
|---|
|
ICC
Use at an assay dependent concentration. |
|
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa. |
Target
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Function
Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. -
Tissue specificity
Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers. -
Sequence similarities
Belongs to the GOLPH3/VPS74 family. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space. - Information by UniProt
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Database links
- Entrez Gene: 78961 Rat
- SwissProt: Q9ERE4 Rat
- Unigene: 64519 Rat
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Alternative names
- Coat protein antibody
- Coat protein GPP34 antibody
- FLJ90675 antibody
see all
Images
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Western blot - Anti-GOLPH3/MIDAS antibody (ab91492)Lane 1 : Anti-GOLPH3/MIDAS antibody (ab91492) at 0.5 µg/ml
Lane 2 : Anti-GOLPH3/MIDAS antibody (ab91492) at 1 µg/ml
All lanes : rat lung tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 34 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa, 42 kDa, 50 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)Image courtesy of Armen Petrosyan by Abreview.ab91492 staining GOLPH3/MIDAS in human K562 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in formaldehyde, blocked with 2% serum for 1 hour at 22°C and then incubated with ab91492 at a 1/150 dilution for 1 hour at 22°C. The secondary used was a DyLight 594 conjugated donkey anti-rabbit polyclonal used at a 1/200 dilution.Blue is nucleus staining by DAPI, red is GOLPH3/MIDAS.
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Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)Image from Bishé B et al., J Biol Chem 287:27637-47 (2012)Fig 6.; doi: 10.1074/jbc.M112.346569; August 10, 2012, The Journal of Biological Chemistry, 287, 27637-27647.ab91492, staining GOLPH3/MIDAS (red) in HCV-infected Huh 7.5.1 cells, by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in 3% paraformaldehyde, permeabilized and blocked in antibody-binding buffer for 2 hours at 4°C. Samples were incubated with primary antibody and a fluorescence-conjugated donkey anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI (blue). -
Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)Image from Sangwung P et al., PLoS One. 2012;7(2):e31564. Epub 2012 Feb 21. Fig 3.; doi:10.1371/journal.pone.0031564; February 21, 2012, PLoS ONE 7(2): e31564.ab91492, staining GOLPH3/MIDAS in bovine aortic endothelial cells, by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100/PBS for 15 min at RT and blocked with 5% donkey serum/0.3% BSA/PBS for 30 min at RT. Samples were incubated with primary antibody at 1/100 dilution overnight at 4°C. An AlexaFlour®568-conjugated anti-rabbit IgG (1/500) was used as the secondary antibody. -
Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)Immunofluorescence of GOLPH3/MIDAS in Rat Lung cells using ab91492 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab91492 has been referenced in 9 publications.
- Li Y et al. Suppressing MDSC Infiltration in Tumor Microenvironment Serves as an Option for Treating Ovarian Cancer Metastasis. Int J Biol Sci 18:3697-3713 (2022). PubMed: 35813475
- Zhao C et al. GOLPH3 Promotes Angiogenesis of Lung Adenocarcinoma by Regulating the Wnt/ß-Catenin Signaling Pathway. Onco Targets Ther 13:6265-6277 (2020). PubMed: 32636646
- Peñalver-González B et al. Golgi Oncoprotein GOLPH3 Gene Expression Is Regulated by Functional E2F and CREB/ATF Promoter Elements. Genes (Basel) 10:N/A (2019). PubMed: 30934642
- Song Q et al. ATF-3/miR-590/GOLPH3 signaling pathway regulates proliferation of breast cancer. BMC Cancer 18:255 (2018). PubMed: 29534690
- Tang S et al. Expression of GOLPH3 in patients with non-small cell lung cancer and xenografts models. Oncol Lett 15:7555-7562 (2018). PubMed: 29849795
- Tang S et al. GOLPH3: a novel biomarker that correlates with poor survival and resistance to chemotherapy in breast cancer. Oncotarget 8:105155-105169 (2017). PubMed: 29285241
- Zhang L et al. Golgi phosphoprotein 3 expression predicts poor prognosis in patients with prostate cancer undergoing radical prostatectomy. Mol Med Rep 12:1298-304 (2015). PubMed: 25760033
- Sangwung P et al. Proteomic identification of S-nitrosylated Golgi proteins: new insights into endothelial cell regulation by eNOS-derived NO. PLoS One 7:e31564 (2012). ICC/IF ; Cow . PubMed: 22363674
- Bishé B et al. Role of Phosphatidylinositol 4-Phosphate (PI4P) and Its Binding Protein GOLPH3 in Hepatitis C Virus Secretion. J Biol Chem 287:27637-47 (2012). ICC/IF ; Human . PubMed: 22745132