Recombinant Anti-GPD2 antibody [EPR14259] (ab188585)
![Western blot - Anti-GPD2 antibody [EPR14259] (ab188585)](https://www.abcam.com/ps/products/188/ab188585/Images/ab188585-222632-anti-gpd2-antibody-epr14259-western-blot.jpg "Western blot - Anti-GPD2 antibody [EPR14259] (ab188585)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14259] to GPD2
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-GPD2 antibody [EPR14259]
See all GPD2 primary antibodies -
Description
Rabbit monoclonal [EPR14259] to GPD2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HeLa cells; HeLa, MCF-7, NIH:OVCAR-3 and U87-MG cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14259 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/310.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (3) |
1/1000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 81 kDa).
|
| IP |
1/30 - 1/50.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/310. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 81 kDa). |
|
IP
1/30 - 1/50. |
Target
-
Pathway
Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1. -
Sequence similarities
Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.
Contains 2 EF-hand domains. -
Cellular localization
Mitochondrion. - Information by UniProt
-
Database links
- Entrez Gene: 2820 Human
- Entrez Gene: 14571 Mouse
- Entrez Gene: 25062 Rat
- Omim: 138430 Human
- SwissProt: P43304 Human
- SwissProt: Q64521 Mouse
- SwissProt: P35571 Rat
- Unigene: 512382 Human
see all -
Alternative names
- GDH2 antibody
- Glycerol 3 phosphate dehydrogenase 2 (mitochondrial) antibody
- Glycerol 3 phosphate dehydrogenase 2 antibody
see all
Images
-
Western blot - Anti-GPD2 antibody [EPR14259] (ab188585)All lanes : Anti-GPD2 antibody [EPR14259] (ab188585) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : NIH:OVCAR-3 cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 81 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-GPD2 antibody [EPR14259] (ab188585)](https://www.abcam.com/ps/products/188/ab188585/Images/ab188585-222631-anti-gpd2-antibody-epr14259-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-GPD2 antibody [EPR14259] (ab188585)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling GPD2 with ab188585 at 1/310 dilution (red) compared to a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
-
![Immunoprecipitation - Anti-GPD2 antibody [EPR14259] (ab188585)](https://www.abcam.com/ps/products/188/ab188585/Images/ab188585-222629-anti-gpd2-antibody-epr14259-immunoprecipitation.jpg)
Immunoprecipitation - Anti-GPD2 antibody [EPR14259] (ab188585)Western blot aalysis of GPD2 in HeLa cell lysate immunoprecipitated using ab188585 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
-
![Anti-GPD2 antibody [EPR14259] (ab188585)](https://www.abcam.com/ps/products/188/ab188585/Images/ab188585-4-benefits-of-recombinant-antibodies.png)
Anti-GPD2 antibody [EPR14259] (ab188585)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (9)
ab188585 has been referenced in 9 publications.
- Ding Y et al. Effect of miR-143-3p from Extracellular Vesicles of Porcine Uterine Luminal Fluid on Porcine Trophoblast Cells. Animals (Basel) 12:N/A (2022). PubMed: 36496922
- Qu H et al. Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease. Diabetes 70:1372-1387 (2021). PubMed: 33741719
- Tan W et al. Improvement of Endothelial Dysfunction of Berberine in Atherosclerotic Mice and Mechanism Exploring through TMT-Based Proteomics. Oxid Med Cell Longev 2020:8683404 (2020). PubMed: 32566106
- Chen J et al. The Potential of the FSP1cre-Pparb/d-/- Mouse Model for Studying Juvenile NAFLD. Int J Mol Sci 20:N/A (2019). PubMed: 31618976
- Thakur S et al. Metformin Targets Mitochondrial Glycerophosphate Dehydrogenase to Control Rate of Oxidative Phosphorylation and Growth of Thyroid Cancer In Vitro and In Vivo. Clin Cancer Res N/A:N/A (2018). PubMed: 29691295
- Liu X et al. Mitochondrial glycerol 3-phosphate dehydrogenase promotes skeletal muscle regeneration. EMBO Mol Med 10:N/A (2018). PubMed: 30389681
- Pinto SK et al. Expression of microRNAs and target proteins in skeletal muscle of rats selectively bred for high and low running capacity. Am J Physiol Endocrinol Metab 313:E335-E343 (2017). PubMed: 28465283
- Pehar M et al. Changes in Protein Expression and Lysine Acetylation Induced by Decreased Glutathione Levels in Astrocytes. Mol Cell Proteomics 15:493-505 (2016). PubMed: 26486419
- Xia M et al. Cell Type-Specific Effects of Mutant DISC1: A Proteomics Study. Mol Neuropsychiatry 2:28-36 (2016). PubMed: 27606318