Recombinant Anti-Granzyme B antibody [BLR022E] (ab243879)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [BLR022E] (ab243879)](https://www.abcam.com/ps/products/243/ab243879/Images/ab243879-334304-anti-granzyme-b-antibody-blr022e-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [BLR022E] (ab243879)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR022E] to Granzyme B
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human
Overview
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Product name
Anti-Granzyme B antibody [BLR022E]
See all Granzyme B primary antibodies -
Description
Rabbit monoclonal [BLR022E] to Granzyme B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Granzyme B.
Database link: P10144 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
Purification notes
The monoclonal antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR022E -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000.
|
|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC |
1/100 - 1/500.
|
| Notes |
|---|
|
WB
1/1000. |
|
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC
1/100 - 1/500. |
Target
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Function
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. -
Sequence similarities
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells. - Information by UniProt
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Database links
- Entrez Gene: 3002 Human
- Omim: 123910 Human
- SwissProt: P10144 Human
- Unigene: 1051 Human
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Alternative names
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [BLR022E] (ab243879)Formalin-fixed, paraffin-embedded human tonsil tissue stained for Ki67 using ab243879 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [BLR022E] (ab243879)](https://www.abcam.com/ps/products/243/ab243879/Images/ab243879-466766-ab-243879-antigranzyme-b-antibody-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [BLR022E] (ab243879)Formalin-fixed, paraffin-embedded human lung carcinoma stained for Granzyme B (red) using ab243879 at 1/250 dilution in immunohistochemical analysis. HRP-conjugated goat anti-rabbit IgG was used as the secondary. Opal™ staining. -
![Western blot - Anti-Granzyme B antibody [BLR022E] (ab243879)](https://www.abcam.com/ps/products/243/ab243879/Images/ab243879-334307-anti-granzyme-b-antibody-blr022e-western-blot.jpg)
Western blot - Anti-Granzyme B antibody [BLR022E] (ab243879)Western blot analysis of Granzyme B using ab243879 at 1/1000 dilution.
Lane 1: SR whole cell lysate cell lysate (50 µg).
Lane 2: SR whole cell lysate cell lysate (15 µg).
Lane 3: SR whole cell lysate cell lysate (5 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds.
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![Western blot - Anti-Granzyme B antibody [BLR022E] (ab243879)](https://www.abcam.com/ps/products/243/ab243879/Images/ab243879-334306-anti-granzyme-b-antibody-blr022e-western-blot.jpg)
Western blot - Anti-Granzyme B antibody [BLR022E] (ab243879)Western blot analysis of MYC-tagged Granzyme B using ab243879 at 1/1000 dilution.
Lane 1: Granzyme A overexpressing overexpressing cell lysate (15 µg).
Lane 2: Granzyme H overexpressing overexpressing cell lysate (15 µg).
Lane 3: Granzyme A overexpressing overexpressing cell lysate (15 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds.
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![Immunocytochemistry - Anti-Granzyme B antibody [BLR022E] (ab243879)](https://www.abcam.com/ps/products/243/ab243879/Images/ab243879-334305-anti-granzyme-b-antibody-blr022e-immunocytochemistry.jpg)
Immunocytochemistry - Anti-Granzyme B antibody [BLR022E] (ab243879)Paraffin-embedded, Formaldehyde-fixed Granzyme B over-expressing cells (Left) and Granzyme A over-expressing cells (right) labeling Granzyme B using ab243879 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining -
![Anti-Granzyme B antibody [BLR022E] (ab243879)](https://www.abcam.com/ps/products/243/ab243879/Images/ab243879-5-benefits-of-recombinant-antibodies.png)
Anti-Granzyme B antibody [BLR022E] (ab243879)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab243879 has not yet been referenced specifically in any publications.