Recombinant Anti-GRB2 antibody [81/GRB2] (ab281846)
![Western blot - Anti-GRB2 antibody [81/GRB2] (ab281846)](https://www.abcam.com/ps/products/281/ab281846/Images/ab281846-471159-anti-grb2-antibody-81grb2-western-blot-hela.jpg "Western blot - Anti-GRB2 antibody [81/GRB2] (ab281846)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [81/GRB2] to GRB2
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GRB2 antibody [81/GRB2]
See all GRB2 primary antibodies -
Description
Mouse monoclonal [81/GRB2] to GRB2 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, A431, NIH/3T3, PC-12, Human tonsil, Mouse testis, Rat testis, Wild type HCT116, 293T lysates.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
81/GRB2 -
Isotype
IgG1 -
Research areas
Associated products
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab281846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000.
|
| Notes |
|---|
|
WB
1/1000. |
Target
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Function
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. -
Sequence similarities
Belongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
Domain
The SH3 domains mediate interaction with RALGPS1 and SHB. -
Cellular localization
Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 2885 Human
- Entrez Gene: 14784 Mouse
- Entrez Gene: 81504 Rat
- Omim: 108355 Human
- SwissProt: P62993 Human
- SwissProt: Q60631 Mouse
- SwissProt: P62994 Rat
- Unigene: 444356 Human
see all -
Alternative names
- Abundant SRC homology antibody
- Adapter protein GRB2 antibody
- ASH antibody
see all
Images
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Western blot - Anti-GRB2 antibody [81/GRB2] (ab281846)All lanes : Anti-GRB2 antibody [81/GRB2] (ab281846) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 5 : Human tonsil tissue lysate
Lane 6 : Mouse testis tissue lysate
Lane 7 : Rat testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Observed band size: 26 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 7 seconds
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![Western blot - Anti-GRB2 antibody [81/GRB2] (ab281846)](https://www.abcam.com/ps/products/281/ab281846/Images/ab281846-471914-anti-grb2-antibody-81grb2-western-blot-wild-type-hct116.jpg)
Western blot - Anti-GRB2 antibody [81/GRB2] (ab281846)All lanes : Anti-GRB2 antibody [81/GRB2] (ab281846) at 1/1000 dilution
Lane 1 : Wild type HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate
Lane 2 : GRB2 knockout HCT116 (ab273715) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
Lane 4 : 293T (human embryonic kidney epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (IRDye® 800CW) (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (ab216777) at 1/10000 dilution
Observed band size: 26 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
Lanes 1-4: Merged signal (red and green). Green - ab281846 observed at 26KDa. Red - loading control ab181602 (Rabbit monoclonal [EPR16891] to GAPDH) observed at 36 kDa.
Lanes 1-2: ab281846 Anti-GRB2 antibody was shown to react with GRB2 in HCT116 cells in Western blot. Loss of signal was observed when GRB2 knockout cell line ab273715 (knockout cell lysate ab275248) was used. Wild-type and GRB2 knockout samples were subjected to SDS-PAGE.
ab281846 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800RD) preadsorbed (ab216772) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging. -
![Anti-GRB2 antibody [81/GRB2] (ab281846)](https://www.abcam.com/ps/products/281/ab281846/Images/ab281846-3-Benefits-of-Recombinant-antibodies.jpg)
Anti-GRB2 antibody [81/GRB2] (ab281846)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab281846 has not yet been referenced specifically in any publications.