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RecombinantRabMAb

Recombinant Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)

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Western blot - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
  • Flow Cytometry (Intracellular) - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
  • Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y301] to GRB2 - BSA and Azide free
  • Suitable for: IHC-P, WB, Flow Cyt (Intra), ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-GRB2 antibody [Y301] - BSA and Azide free
    See all GRB2 primary antibodies

  • Description

    Rabbit monoclonal [Y301] to GRB2 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognises GRB2. It can also detect the splice isoform GRB3-3 of GRB2.

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt (Intra), ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247231 is the carrier-free version of ab32111.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Application notes
Is unsuitable for IP.

Target

  • Function

    Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
    Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.

  • Sequence similarities

    Belongs to the GRB2/sem-5/DRK family.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.

  • Domain

    The SH3 domains mediate interaction with RALGPS1 and SHB.

  • Cellular localization

    Golgi apparatus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Abundant SRC homology antibody
    • Adapter protein GRB2 antibody
    • ASH antibody
    • Ash protein antibody
    • EGFRBP GRB2 antibody
    • Epidermal growth factor receptor binding protein antibody
    • Epidermal growth factor receptor binding protein GRB2 antibody
    • GRB 2 antibody
    • GRB2 adapter protein antibody
    • Grb2 antibody
    • GRB2_HUMAN antibody
    • Grb3 3 antibody
    • Growth factor receptor bound protein 2 antibody
    • Growth factor receptor bound protein 3 antibody
    • Growth factor receptor-bound protein 2 antibody
    • HT027 antibody
    • MST084 antibody
    • MSTP084 antibody
    • NCKAP2 antibody
    • OTTHUMP00000166096 antibody
    • OTTHUMP00000166097 antibody
    • OTTHUMP00000166098 antibody
    • Protein ash antibody
    • SEM5 antibody
    • SH2/SH3 adapter GRB2 antibody
    see all

Images

  • Western blot - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
    Western blot - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
    All lanes : Anti-GRB2 antibody [Y301] (ab32111) at 1/5000 dilution (purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 4 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 5 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    This data was developed using ab32111, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    We are unsure how to define the extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
    This data was developed using ab32111, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of GRB2 expression in paraffin embedded human breast carcinoma tissue section, using 1/100 ab32111. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry/ Immunofluorescence - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
    Immunocytochemistry/ Immunofluorescence - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
    This data was developed using ab32111, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of GRB2 expression in PC12 cells, using 1/100 ab32111.
  • Flow Cytometry (Intracellular) - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
    Flow Cytometry (Intracellular) - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
    This data was developed using ab32111, the same antibody clone in a different buffer formulation.Overlay histogram showing SH-SY5Y cells stained with ab32111 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32111, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
    Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab247231? Please let us know so that we can cite the reference in this datasheet.

ab247231 has not yet been referenced specifically in any publications.

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