Anti-GRIM19 antibody [6E1BH7] (ab110240)
![Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240)](https://www.abcam.com/ps/products/110/ab110240/Images/ab110240-393562-anti-grim19-antibody-6e1bh7-western-blot-human-knockout-lysate.jpg "Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240)")
Key features and details
- Mouse monoclonal [6E1BH7] to GRIM19
- Suitable for: WB, ICC/IF, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-GRIM19 antibody [6E1BH7]
See all GRIM19 primary antibodies -
Description
Mouse monoclonal [6E1BH7] to GRIM19 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Recombinant full length protein. This information is considered to be commercially sensitive.
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Positive control
- WB: HeLa and Jurkat cell lysates; Human heart, Bovine heart, Rat heart, and Mouse heart isolated mitochondria. ICC/IF: Human fibroblasts. Flow Cyt: HeLa cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purification notes
ab110240 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
6E1BH7 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110240 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
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| ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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Flow Cyt
Use a concentration of 1 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes. -
Tissue specificity
Widely expressed, with highest expression in heart, skeletal muscle, liver, kidney and placenta. In intestinal mucosa, down-regulated in areas involved in Crohn disease and ulcerative colitis. -
Involvement in disease
Defects in NDUFA13 may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms. -
Sequence similarities
Belongs to the complex I NDUFA13 subunit family. -
Developmental stage
Expressed in numerous fetal tissues. -
Cellular localization
Mitochondrion inner membrane. Nucleus. May be translocated into the nucleus upon IFN/RA treatment. - Information by UniProt
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Database links
- Entrez Gene: 338084 Cow
- Entrez Gene: 51079 Human
- Entrez Gene: 67184 Mouse
- Entrez Gene: 314759 Rat
- Omim: 609435 Human
- SwissProt: Q95KV7 Cow
- SwissProt: Q9P0J0 Human
- SwissProt: Q9ERS2 Mouse
see all -
Alternative names
- 2700054G14Rik antibody
- AU022060 antibody
- B16.6 antibody
see all
Images
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Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240)All lanes : Anti-GRIM19 antibody [6E1BH7] (ab110240) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : NDUFA13 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDaLanes 1-3: Merged signal (red and green). Green - ab110240 observed at 17 kDa. Red - loading control ab181602 observed at 36 kDa.
ab110240 GRIM19 antibody [6E1BH7] was shown to specifically react with GRIM19 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265863 (knockout cell lysate ab257136) was used. Wild-type and GRIM19 knockout samples were subjected to SDS-PAGE. ab110240 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240)](https://www.abcam.com/ps/products/110/ab110240/Images/ab110240-123414-anti-grim19-antibody-6e1bh7-western-blot.jpg)
Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240)All lanes : Anti-GRIM19 antibody [6E1BH7] (ab110240) at 1 µg/ml
Lane 1 : Human heart mitochondria
Lane 2 : Bovine heart mitochondria
Lane 3 : Rat heart mitochondria
Lane 4 : Mouse heart mitochondria
Predicted band size: 17 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-GRIM19 antibody [6E1BH7] (ab110240)](https://www.abcam.com/ps/products/110/ab110240/Images/ab110240-123413-anti-grim19-antibody-6e1bh7-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-GRIM19 antibody [6E1BH7] (ab110240)Mitochondrial localization of GRIM19 visualized by immunocytochemistry using ab110240 at a concentration of 1 µg/mL. Cultured Human fibroblasts were fixed, permeabilized and then labeled with ab110240 followed by Alexa® 488 goat-anti-mouse IgG. -
![Flow Cytometry - Anti-GRIM19 antibody [6E1BH7] (ab110240)](https://www.abcam.com/ps/products/110/ab110240/Images/ab110240-123412-anti-grim19-antibody-6e1bh7-flow-cytometry.jpg)
Flow Cytometry - Anti-GRIM19 antibody [6E1BH7] (ab110240)HeLa cells were stained with 1 µg/mL ab110240 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
Datasheets and documents
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SDS download
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Datasheet download
References (46)
ab110240 has been referenced in 46 publications.
- Yu M et al. Berberine alleviates lipid metabolism disorders via inhibition of mitochondrial complex I in gut and liver. Int J Biol Sci 17:1693-1707 (2021). PubMed: 33994854
- Jhun J et al. GRIM19 Impedes Obesity by Regulating Inflammatory White Fat Browning and Promoting Th17/Treg Balance. Cells 10:N/A (2021). PubMed: 33467683
- Liu XD et al. Metformin protects high glucose-cultured cardiomyocytes from oxidative stress by promoting NDUFA13 expression and mitochondrial biogenesis via the AMPK signaling pathway. Mol Med Rep 22:5262-5270 (2020). PubMed: 33174032
- Formosa LE et al. Dissecting the Roles of Mitochondrial Complex I Intermediate Assembly Complex Factors in the Biogenesis of Complex I. Cell Rep 31:107541 (2020). PubMed: 32320651
- Zhao QY et al. Assessment of mitochondrial function in metabolic dysfunction-associated fatty liver disease using obese mouse models. Zool Res 41:539-551 (2020). PubMed: 32786176