Recombinant Anti-GRIM19 antibody [EPR4471(2)] (ab109017)
![Western blot - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)](https://www.abcam.com/ps/products/109/ab109017/Images/ab109017-393564-anti-grim19-antibody-epr44712-western-blot-human-knockout-lysate.jpg "Western blot - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4471(2)] to GRIM19
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GRIM19 antibody [EPR4471(2)]
See all GRIM19 primary antibodies -
Description
Rabbit monoclonal [EPR4471(2)] to GRIM19 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Ramos, Jurkat, and 293T cell lysates. IHC-P: Human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4471(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 17 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes. -
Tissue specificity
Widely expressed, with highest expression in heart, skeletal muscle, liver, kidney and placenta. In intestinal mucosa, down-regulated in areas involved in Crohn disease and ulcerative colitis. -
Involvement in disease
Defects in NDUFA13 may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms. -
Sequence similarities
Belongs to the complex I NDUFA13 subunit family. -
Developmental stage
Expressed in numerous fetal tissues. -
Cellular localization
Mitochondrion inner membrane. Nucleus. May be translocated into the nucleus upon IFN/RA treatment. - Information by UniProt
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Database links
- Entrez Gene: 51079 Human
- Omim: 609435 Human
- SwissProt: Q9P0J0 Human
- Unigene: 534453 Human
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Alternative names
- 2700054G14Rik antibody
- AU022060 antibody
- B16.6 antibody
see all
Images
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Western blot - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)All lanes : Anti-GRIM19 antibody [EPR4471(2)] (ab109017) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : NDUFA13 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDaLanes 1-3: Merged signal (red and green). Green - ab109017 observed at 17 kDa. Red - loading control ab8245 observed at 36 kDa.
ab109017 Anti-GRIM19 antibody [EPR4471(2)] was shown to specifically react with GRIM19 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265863 (knockout cell lysate ab257136) was used. Wild-type and GRIM19 knockout samples were subjected to SDS-PAGE. ab109017 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)](https://www.abcam.com/ps/products/109/ab109017/Images/ab109017-121590-anti-grim19-antibody-epr44712-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)Immunohistochemical analysis of GRIM19 in paraffin-embedded Human kidney tissue using ab109017 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)](https://www.abcam.com/ps/products/109/ab109017/Images/ab109017-121591-anti-grim19-antibody-epr44712-western-blot.jpg)
Western blot - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)All lanes : Anti-GRIM19 antibody [EPR4471(2)] (ab109017) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 17 kDa -
![Anti-GRIM19 antibody [EPR4471(2)] (ab109017)](https://www.abcam.com/ps/products/109/ab109017/Images/ab109017-4-benefits-of-recombinant-antibodies.png)
Anti-GRIM19 antibody [EPR4471(2)] (ab109017)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab109017 has not yet been referenced specifically in any publications.