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Anti-groEL antibody (ab90522)

Reviews (2)Q&A (4)References (15)
Western blot - Anti-groEL antibody (ab90522)

    Key features and details

    • Rabbit polyclonal to groEL
    • Suitable for: WB
    • Reacts with: Escherichia coli
    • Isotype: IgG

    Overview

    • Product name

      Anti-groEL antibody
      See all groEL primary antibodies

    • Description

      Rabbit polyclonal to groEL

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Escherichia coli

    • Immunogen

      Full length protein corresponding to Escherichia coli groEL. Strain K12
      Database link: P0A6F5

    • Positive control

      • WB: Recombinant groEL protein; E.coli cell lysate.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab90522 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (2)
    1/1000. Predicted molecular weight: 57 kDa.
    Notes
    WB
    1/1000. Predicted molecular weight: 57 kDa.

    Target

    • Relevance

      The bacterial chaperonin groEL is a double toroidal assembly, which together with the action of the ring-shaped oligomeric cochaperonin, GroES, facilitates protein folding in an ATP dependent manner.

    • Cellular localization

      Cytoplasmic

    • Database links

      • Alternative names

        • 60 kDa chaperonin antibody
        • groEL protein antibody
        • groL antibody
        • mopA antibody
        • Protein Cpn60 antibody
        see all

      Images

      • Western blot - Anti-groEL antibody (ab90522)
        Western blot - Anti-groEL antibody (ab90522)
        All lanes : Anti-groEL antibody (ab90522) at 1/1000 dilution

        Lane 1 : groEL recombinant protein
        Lane 2 : Human recombinant HSP60
        Lane 3 : Heat Shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
        Lane 4 : E.coli cell lysate

        Predicted band size: 57 kDa

      References (15)

      Publishing research using ab90522? Please let us know so that we can cite the reference in this datasheet.

      ab90522 has been referenced in 15 publications.

      • Northrop-Albrecht EJ  et al. Assessment of extracellular vesicle isolation methods from human stool supernatant. J Extracell Vesicles 11:e12208 (2022). PubMed: 35383410
      • Kim JS  et al. PE_PGRS38 Interaction With HAUSP Downregulates Antimycobacterial Host Defense via TRAF6. Front Immunol 13:862628 (2022). PubMed: 35572598
      • Borgert SR  et al. Moonlighting chaperone activity of the enzyme PqsE contributes to RhlR-controlled virulence of Pseudomonas aeruginosa. Nat Commun 13:7402 (2022). PubMed: 36456567
      • Zhang X  et al. Bis-molybdopterin guanine dinucleotide modulates hemolysin expression under anaerobiosis and contributes to fitness in vivo in uropathogenic Escherichia coli. Mol Microbiol 116:1216-1231 (2021). PubMed: 34494331
      • Lagha R  et al. Effect of Plasmonic Gold Nanoprisms on Biofilm Formation and Heat Shock Proteins Expression in Human Pathogenic Bacteria. Pharmaceuticals (Basel) 14:N/A (2021). PubMed: 34959736
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-6 of 6 Abreviews or Q&A

      Western blot abreview for Anti-groEL antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Pseudomonas aeruginosa Cell lysate - whole cell (pseudomonas aeruginosa)
      Gel Running Conditions
      Non-reduced Denaturing
      Loading amount
      1e+006 cells
      Specification
      pseudomonas aeruginosa
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Yu Zhang

      Verified customer

      Submitted Jan 12 2016

      Western blot abreview for Anti-groEL antibody

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      75 µg
      Gel Running Conditions
      Reduced Denaturing (18)
      Sample
      Escherichia coli Cell lysate - whole cell (E. coli Deletion variants)
      Specification
      E. coli Deletion variants
      Treatment
      20 mM Nitrat, no oxygen for 16 hrs
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Oct 14 2014

      Question

      I will go ahead with the order with the potential for a future discount if the antibody doesn't work as hoped. Thanks for this offer, I appreciate it. I'll await your reply before I order anything.

      Read More
      Answer


      I am very pleased to hear you would like to accept our offer and test ab90522 in P. aerugionsa. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for P. aerugionsa and include this code in the Additional Comments section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.


      For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Question

      I was curious about product ab90522.

      Read More
      Answer

      Thank you very much for your interest in ab90522.

      To our knowledge, ab90522 has not been tested in P. aerugionsa. Therefore, I can offer a discount off a future purchase if you buy ab90522 now, test it in P. aerugionsa and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.
      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know that you would like to proceed and test ab90522 in P. aerugionsa. I will then send a discount code. This code must be issued before purchasing ab90522 so please wait for my reply before ordering.

      2. Purchase ab90522 either by phone, fax, or online (www.abcam.com).

      3. Test it in P. aerugionsa.

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
      Please remember that submission of the Abreview is sufficient for the discount code to become active.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab90522 turns out to be unsuitable for P. aerugionsa, you will still receive the discount on your next purchase after your Abreview has been submitted.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      Read More

      Question

      I need to detect groEL protein in Streptococcus pneumoniae. I was wondering if your Anti-groEL antibody will work.
      Thank you

      Read More
      Answer

      Thank you for contacting Abcam regarding ab90522.


      We have not tested this cross reactivity specifically so I did an alignment. Based on a sequence alignment in Uniprot, there is only about 60% homology between various strains of E. Coli GroEL and streptococcus pneumoniae GroEL so unfortunately there is not a chance of cross reactivity.


      I am sorry for any inconvenience this may cause. Please do not hesitate to contact me if you have any additional questions.

      Read More

      Question

      WB with E.coli
      sees 60 kDa band in LB media (neg control) as well as in lysate
      60 kDa band is expected, but not in filtered LB media
      both bands (media and lysate)have same intensity
      lysate: tris-glycine buffer, boiled, laemmli to denature E.coli proteins
      Ab: 1/1000, 1/2000, 1/3000 1h RT
      block: commercial blocking reagent
      secondary: OK

      Read More
      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab82592.

      To check the status of the order please contact our Customer Service team and reference this number.

      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com