Anti-groEL antibody (ab90522)
Key features and details
- Rabbit polyclonal to groEL
- Suitable for: WB
- Reacts with: Escherichia coli
- Isotype: IgG
Overview
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Product name
Anti-groEL antibody
See all groEL primary antibodies -
Description
Rabbit polyclonal to groEL -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Full length protein corresponding to Escherichia coli groEL. Strain K12
Database link: P0A6F5 -
Positive control
- WB: Recombinant groEL protein; E.coli cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab90522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000. Predicted molecular weight: 57 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 57 kDa. |
Target
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Relevance
The bacterial chaperonin groEL is a double toroidal assembly, which together with the action of the ring-shaped oligomeric cochaperonin, GroES, facilitates protein folding in an ATP dependent manner. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 1037522 Escherichia coli
- SwissProt: P0A6F5 Escherichia coli
- SwissProt: P0A6F6 Escherichia coli
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Alternative names
- 60 kDa chaperonin antibody
- groEL protein antibody
- groL antibody
see all
Images
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All lanes : Anti-groEL antibody (ab90522) at 1/1000 dilution
Lane 1 : groEL recombinant protein
Lane 2 : Human recombinant HSP60
Lane 3 : Heat Shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 4 : E.coli cell lysate
Predicted band size: 57 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab90522 has been referenced in 15 publications.
- Northrop-Albrecht EJ et al. Assessment of extracellular vesicle isolation methods from human stool supernatant. J Extracell Vesicles 11:e12208 (2022). PubMed: 35383410
- Kim JS et al. PE_PGRS38 Interaction With HAUSP Downregulates Antimycobacterial Host Defense via TRAF6. Front Immunol 13:862628 (2022). PubMed: 35572598
- Borgert SR et al. Moonlighting chaperone activity of the enzyme PqsE contributes to RhlR-controlled virulence of Pseudomonas aeruginosa. Nat Commun 13:7402 (2022). PubMed: 36456567
- Zhang X et al. Bis-molybdopterin guanine dinucleotide modulates hemolysin expression under anaerobiosis and contributes to fitness in vivo in uropathogenic Escherichia coli. Mol Microbiol 116:1216-1231 (2021). PubMed: 34494331
- Lagha R et al. Effect of Plasmonic Gold Nanoprisms on Biofilm Formation and Heat Shock Proteins Expression in Human Pathogenic Bacteria. Pharmaceuticals (Basel) 14:N/A (2021). PubMed: 34959736