Anti-Grp75/MOT antibody (ab53098)
Key features and details
- Rabbit polyclonal to Grp75/MOT
- Suitable for: ELISA, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-Grp75/MOT antibody
See all Grp75/MOT primary antibodies -
Description
Rabbit polyclonal to Grp75/MOT -
Host species
Rabbit -
Specificity
Detects endogenous levels of total Grp75 / MOT protein -
Tested applications
Suitable for: ELISA, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, African green monkey -
Immunogen
Synthetic peptide corresponding to Human Grp75/MOT aa 630-679.
Database link: P38646 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| WB | (1) |
1/300 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
|
| IHC-P | (1) |
Use at an assay dependent concentration.
|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
WB
1/300 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
|
ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. -
Sequence similarities
Belongs to the heat shock protein 70 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 3313 Human
- Entrez Gene: 15526 Mouse
- Entrez Gene: 291671 Rat
- Omim: 600548 Human
- SwissProt: P38646 Human
- SwissProt: P38647 Mouse
- SwissProt: P48721 Rat
- Unigene: 184233 Human
see all -
Alternative names
- 75 kDa glucose regulated protein antibody
- 75 kDa glucose-regulated protein antibody
- CSA antibody
see all
Images
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Western blot - Anti-Grp75/MOT antibody (ab53098)All lanes : Anti-Grp75/MOT antibody (ab53098) at 1/300 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 2 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract with peptide
Predicted band size: 74 kDa
Observed band size: 74 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody (ab53098)Immunohistochemical analysis of Grp75/MOT in paraffin-embedded human breast carcinoma tissue, using ab53098 at 1/50 dilution, in the presence (right) or absence (left) of peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody (ab53098)Immunocytochemistry/ Immunofluorescence analysis of Grp75/MOT in COS-7 (African green monkey kidney fibroblast-like) cells, using ab53098 at 1/100 dilution, in the presence (right) or absence (left) of peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody (ab53098)ICC/IF image of ab53098 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53098, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab53098 has been referenced in 21 publications.
- Pilotto F et al. PolyGA targets the ER stress-adaptive response by impairing GRP75 function at the MAM in C9ORF72-ALS/FTD. Acta Neuropathol 144:939-966 (2022). PubMed: 36121477
- Silva-Pinheiro P et al. DNA polymerase gamma mutations that impair holoenzyme stability cause catalytic subunit depletion. Nucleic Acids Res 49:5230-5248 (2021). PubMed: 33956154
- Acín-Perez R et al. Recruitment and remodeling of peridroplet mitochondria in human adipose tissue. Redox Biol 46:102087 (2021). PubMed: 34411987
- Ramraj SK et al. Novel ovarian cancer maintenance therapy targeted at mortalin and mutant p53. Int J Cancer 147:1086-1097 (2020). PubMed: 31845320
- Nomoto H et al. Activation of the HIF1a/PFKFB3 stress response pathway in beta cells in type 1 diabetes. Diabetologia 63:149-161 (2020). PubMed: 31720731