Recombinant Anti-GST antibody [6G9] (ab252882)
![Western blot - Anti-GST antibody [6G9] (ab252882)](https://www.abcam.com/ps/products/252/ab252882/Images/ab252882-391689-anti-gst-antibody-6g9-western-blot-hek293t.jpg "Western blot - Anti-GST antibody [6G9] (ab252882)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [6G9] to GST
- Suitable for: IP, WB
- Reacts with: Species independent
Related conjugates and formulations
Overview
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Product name
Anti-GST antibody [6G9]
See all GST primary antibodies -
Description
Rat monoclonal [6G9] to GST -
Host species
Rat -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant fragment within GST. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a GST-tagged Protein L-Myc (P12524; aa 1-394; 43kDa) construct. whole cell lysate. IP: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a GST-tagged Peroxiredoxin 5 (P30044; aa 1-214; 22kDa) construct whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
6G9 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/30.
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| WB |
1/1000. Predicted molecular weight: 25 kDa.
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| Notes |
|---|
|
IP
1/30. |
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WB
1/1000. Predicted molecular weight: 25 kDa. |
Target
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Relevance
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
Alternative names
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
Images
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Western blot - Anti-GST antibody [6G9] (ab252882)All lanes : Anti-GST antibody [6G9] (ab252882) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty plasmid. whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a GST-tagged Protein L-Myc (P12524; aa 1-394; 43kDa) construct. whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/10000 dilution
Predicted band size: 25 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 59 seconds.
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![Immunoprecipitation - Anti-GST antibody [6G9] (ab252882)](https://www.abcam.com/ps/products/252/ab252882/Images/ab252882-1-anti-gst-antibody-6g9-immunoprecipitation-hek293t.jpg)
Immunoprecipitation - Anti-GST antibody [6G9] (ab252882)GST was immunoprecipitated from 0.35 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a GST-tagged Peroxiredoxin 5 (P30044; aa 1-214; 22kDa) construct whole cell lysate 10µg with ab252882 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252882 at 1/1000 dilution. Goat Anti-Rat IgG (H+L), HRP) (ab205720) was used at 1/5000 dilution.
Lane 1: HEK-293T transfected with a GST-tagged Peroxiredoxin 5 (P30044; aa 1-214; 22kDa) construct whole cell lysate 10µg.
Lane 2: ab252882 IP in HEK-293T transfected with a GST-tagged Peroxiredoxin 5 (P30044; aa 1-214; 22kDa) construct whole cell lysate.
Lane 3: Rat monoclonal IgG (ab18450) instead of ab252882 in HEK-293T transfected with a GST-tagged Peroxiredoxin 5 (P30044; aa 1-214; 22kDa)construct whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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![Anti-GST antibody [6G9] (ab252882)](https://www.abcam.com/ps/products/252/ab252882/Images/ab252882-3-benefits-of-recombinant-antibodies.png)
Anti-GST antibody [6G9] (ab252882)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab252882 has not yet been referenced specifically in any publications.