Anti-GST antibody (ab184804)
Key features and details
- Rabbit polyclonal to GST
- Suitable for: WB, IP, ICC, ELISA
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-GST antibody
See all GST primary antibodies -
Description
Rabbit polyclonal to GST -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Full length protein corresponding to Schistosoma japonicum GST.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/30000. Predicted molecular weight: 25 kDa.
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| IP |
Use a concentration of 1 - 4 µg/ml.
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| ICC |
1/100 - 1/400.
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| ELISA |
1/1000 - 1/30000.
for coating plates use a dilution of 1:100-1:500 |
| Notes |
|---|
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WB
1/1000 - 1/30000. Predicted molecular weight: 25 kDa. |
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IP
Use a concentration of 1 - 4 µg/ml. |
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ICC
1/100 - 1/400.
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ELISA
1/1000 - 1/30000. for coating plates use a dilution of 1:100-1:500 |
Target
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Relevance
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
Alternative names
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
Images
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Western blot - Anti-GST antibody (ab184804)
Detection of GST-tagged Protein by Western Blot. Samples: 0.1, 0.3, or 1.0.µg of 293 cell lysate expressing a GST-tag fusion protein. Antibodies: Affinity purified, rabbit anti-GST antibody ab184804 used for WB at 0.06 µg/ml (1:15,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab184804 has not yet been referenced specifically in any publications.