Anti-GST antibody (ab9085)
Key features and details
- Rabbit polyclonal to GST
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-GST antibody
See all GST primary antibodies -
Description
Rabbit polyclonal to GST -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Constituent: 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified against bacterially expressed GST -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/500.
|
| Notes |
|---|
|
WB
1/500. |
Target
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Relevance
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
Alternative names
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
Images
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Western blot - Anti-GST antibody (ab9085)This image is courtesy of an anonymous AbreviewAll lanes : Anti-GST antibody (ab9085) at 1/2000 dilution
Lane 1 : Recombinant GST protein purified from E. coli XL1-Blue
Lane 2 : Recombinant GST-tagged p53 protein purified from E. coli XL1-Blue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit IgG polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 26,80 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
Blocked with 5% Milk for 1 hour at 25°C.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (59)
ab9085 has been referenced in 59 publications.
- Jaiswal N et al. Regulation of plant immunity and growth by tomato receptor-like cytoplasmic kinase TRK1. New Phytol 233:458-478 (2022). PubMed: 34655240
- Addicks GC et al. GCN5 maintains muscle integrity by acetylating YY1 to promote dystrophin expression. J Cell Biol 221:N/A (2022). PubMed: 35024765
- Shi S et al. Protein tyrosine phosphatase PTPN2 regulates TGF-β signaling through Smad4 dephosphorylation. Am J Cancer Res 12:5516-5531 (2022). PubMed: 36628288
- Peng H et al. Kinetic Characterization of ASXL1/2-Mediated Allosteric Regulation of the BAP1 Deubiquitinase. Mol Cancer Res 19:1099-1112 (2021). PubMed: 33731362
- Park SY et al. Reduction in MLKL-mediated endosomal trafficking enhances the TRAIL-DR4/5 signal to increase cancer cell death. Cell Death Dis 11:744 (2020). PubMed: 32917855