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RecombinantRabMAb

Recombinant Anti-GST antibody [EPR4236] (ab111947)

Submit a review Submit a question References (19)
Immunocytochemistry/ Immunofluorescence - Anti-GST antibody [EPR4236] (ab111947)
  • Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)
  • Western blot - Anti-GST antibody [EPR4236] (ab111947)
  • Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)
  • Western blot - Anti-GST antibody [EPR4236] (ab111947)
  • Anti-GST antibody [EPR4236] (ab111947)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4236] to GST
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Species independent

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-GST antibody [EPR4236]
    See all GST primary antibodies

  • Description

    Rabbit monoclonal [EPR4236] to GST

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide (GST-tag) within GST aa 300-450. The exact sequence is proprietary. GST tag was fused to ATG16L1 to produce a recombinant protein.
    Database link: Q676U5

  • Positive control

    • GST fusion proteins; Alpha-actinin-4-GST, AKT1/PKBa-GST or CDK7-GST.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab111947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
ICC/IF
1/100.
IP
1/20.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
ICC/IF
1/100.
IP
1/20.
Application notes
Is unsuitable for Flow Cyt or IHC-P.

Target

  • Relevance

    GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

  • Alternative names

    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-GST antibody [EPR4236] (ab111947)
    Immunocytochemistry/ Immunofluorescence - Anti-GST antibody [EPR4236] (ab111947)

    Immunofluorescence staining of 293T cells transfected with GST, with ab111947 at a working dilution of 1/100 and counter-stained with DAPI. Tubulin was also stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120). The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1/500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X-100. The negative controls are shown in the bottom hand panels - for the first negative control, anti-GST antibody (ab111947) was used at a dilution of 1/100 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/1000 and for the second negative control, mouse anti-tubulin (ab7291) and anti-rabbit secondary antibody (ab150077) were used.

  • Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)
    Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)

    GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.

    Lane 1: Primary: ab111947, 1/1000 dilution
    Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
    Lane 2: Primary: ab111947, 1/1000 dilution
    Sample: 293T transfected with GST tagged protein vector cell lysate
    Lane 3: Primary: ab172730, Rabbit monoclonal IgG
    Sample: 293T transfected with GST tagged protein vector cell lysate

    All lanes: Secondary: ab131366, Veriblot, 1/10000 dilution
    Blocking and diluting buffer: 5% NFDM/TBST



    Lanes 1-2 : Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution
    Lane 3 : Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    Lane 1 : Input - 293 transfected with GST tagged protein vector cell lysate at 10 µg
    Lane 2 : (+) 293T transfected with GST tagged protein vector cell lysate
    Lane 3 : (-) 293T transfected with GST tagged protein vector cell lysate

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution (VeriBlot)

    Additional bands at: 41 kDa (possible tagged protein)

  • Western blot - Anti-GST antibody [EPR4236] (ab111947)
    Western blot - Anti-GST antibody [EPR4236] (ab111947)
    Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution + GST recombinant protein at 0.01 µg

    Predicted band size: 26 kDa

  • Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)
    Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)

    (Cross IP) GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.

    Lane 1: Primary: ab180123, 1/1000 dilution
    Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
    Lane 2: Primary: ab180123, 1/1000 dilution
    Sample: 293T transfected with GST tagged protein vector cell lysate
    Secondary: ab131366, Veriblot, 1/10000 dilution
    Lane 3: Primary: ab172730, Rabbit monoclonal IgG
    Sample: 293T transfected with GST tagged protein vector cell lysate

    All lanes: Secondary: ab131366, Veriblot, 1/10000 dilution
    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-GST antibody [EPR4236] (ab111947)
    Western blot - Anti-GST antibody [EPR4236] (ab111947)
    All lanes : Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution

    Lane 1 : Alpha-actinin-4-GST fusion protein
    Lane 2 : AKT1/PKBa-GST fusion protein
    Lane 3 : CDK7-GST fusion protein

    Lysates/proteins at 0.01 µg per lane.

    Predicted band size: 26 kDa

  • Anti-GST antibody [EPR4236] (ab111947)
    Anti-GST antibody [EPR4236] (ab111947)

References (19)

Publishing research using ab111947? Please let us know so that we can cite the reference in this datasheet.

ab111947 has been referenced in 19 publications.

  • Yang G  et al. The Effector Protein CgNLP1 of Colletotrichum gloeosporioides Affects Invasion and Disrupts Nuclear Localization of Necrosis-Induced Transcription Factor HbMYB8-Like to Suppress Plant Defense Signaling. Front Microbiol 13:911479 (2022). PubMed: 35770165
  • Qiu Z  et al. PLK1-mediated phosphorylation of PPIL2 regulates HR via CtIP. Front Cell Dev Biol 10:902403 (2022). PubMed: 36092721
  • Chen L  et al. Mangiferin prevents myocardial infarction-induced apoptosis and heart failure in mice by activating the Sirt1/FoxO3a pathway. J Cell Mol Med 25:2944-2955 (2021). PubMed: 33523605
  • Xu Q  et al. Aloin protects mice from diet-induced non-alcoholic steatohepatitis via activation of Nrf2/HO-1 signaling. Food Funct 12:696-705 (2021). PubMed: 33410857
  • Xu Q  et al. Adenosine 5'-monophosphate-activated protein kinase ameliorates bovine adipocyte oxidative stress by inducing antioxidant responses and autophagy. J Dairy Sci 104:4516-4528 (2021). PubMed: 33551161
View all Publications for this product

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