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RecombinantRabMAb

Recombinant Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)

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Immunoprecipitation - Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)
  • Immunoprecipitation - Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)
  • Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4236] to GST - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Species independent

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-GST antibody [EPR4236] - BSA and Azide free
    See all GST primary antibodies

  • Description

    Rabbit monoclonal [EPR4236] to GST - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide (GST-tag) within GST aa 300-450. The exact sequence is proprietary. GST tag was fused to ATG16L1 to produce a recombinant protein.
    Database link: Q676U5

  • General notes

    ab235384 is the carrier-free version of ab111947.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab235384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Application notes
Is unsuitable for Flow Cyt or IHC-P.

Target

  • Relevance

    GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

  • Alternative names

    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Images

  • Immunoprecipitation - Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)
    Immunoprecipitation - Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)

    (Cross IP) GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.

    Lane 1: Primary: ab180123, 1/1000 dilution
    Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
    Lane 2: Primary: ab180123, 1/1000 dilution
    Sample: 293T transfected with GST tagged protein vector cell lysate
    Secondary: ab131366, Veriblot, 1/10000 dilution
    Lane 3: Primary: ab172730, Rabbit monoclonal IgG
    Sample: 293T transfected with GST tagged protein vector cell lysate

    All lanes: VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
    Blocking and diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111947).

  • Immunoprecipitation - Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)
    Immunoprecipitation - Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)

    GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.

    Lane 1: Primary: ab111947, 1/1000 dilution
    Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
    Lane 2: Primary: ab111947, 1/1000 dilution
    Sample: 293T transfected with GST tagged protein vector cell lysate
    Lane 3: Primary: ab172730, Rabbit monoclonal IgG
    Sample: 293T transfected with GST tagged protein vector cell lysate

    All lanes: VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
    Blocking and diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111947).

  • Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)
    Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab235384? Please let us know so that we can cite the reference in this datasheet.

ab235384 has not yet been referenced specifically in any publications.

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