Anti-GST Tag Antibody (ab307273)
Key features and details
- Rabbit polyclonal to GST
- Suitable for: WB, ICC/IF
- Isotype: IgG
Overview
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Product name
Anti-GST Tag Antibody
See all GST primary antibodies -
Description
Rabbit polyclonal to GST -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Immunogen
Recombinant full length protein corresponding to Schistosoma japonicum GST.
Database link: P08515 -
Positive control
- WB: Whole cell lysate from CHO cells transfected with pcDNA3-12 Tags. ICC/IF: CHO cells transfected with 12-tags constructs.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab307273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 25 kDa.
|
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| ICC/IF |
1/2000.
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| Notes |
|---|
|
WB
1/2000. Predicted molecular weight: 25 kDa. |
|
ICC/IF
1/2000. |
Target
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Relevance
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
Alternative names
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GST Tag Antibody (ab307273)
Immunocytochemistry/ Immunofluorescence analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized CHO cells transfected with 12-tags constructs labeling GST-Tag with AB307273 at a 1/2000 dilution (0.5 µg/ml) followed by Donkey anti-rabbit IgG (H&L) conjugated to FITC at a 1/1000 dilution (1 µg/ml) (Green). Nuclei stained with DAPI (Blue).
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Western blot - Anti-GST Tag Antibody (ab307273)All lanes : Anti-GST Tag Antibody (ab307273) at 1/2000 dilution
Lane 1 : CHO cells transfected with pcDNA3-12 Tags
Lane 2 : Non-transfected CHO cells
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Donkey anti-rabbit IgG at 1/5000 dilution
Predicted band size: 25 kDaCells incubated with primary antibody for 1 hour at room temperature followed by the secondary antibody for 1 hour at room temperature.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab307273 has not yet been referenced specifically in any publications.