Recombinant Anti-GSTK1 antibody [EP1938Y] (ab52759)
![Western blot - Anti-GSTK1 antibody [EP1938Y] (ab52759)](https://www.abcam.com/ps/products/52/ab52759/Images/ab52759-393622-anti-gstk1-antibody-ep1938y-western-blot-human-knockout-lysate.jpg "Western blot - Anti-GSTK1 antibody [EP1938Y] (ab52759)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1938Y] to GSTK1
- Suitable for: ICC/IF, WB, IP, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GSTK1 antibody [EP1938Y]
See all GSTK1 primary antibodies -
Description
Rabbit monoclonal [EP1938Y] to GSTK1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human GSTK1 aa 150-250 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HEK293T, HeLa and Daudi cell lysates; Fetal liver lysate. IHC-P: Human liver tissue. ICC: A549 cells. IP: Human heart lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1938Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/250.
|
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| WB |
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
|
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| IP |
1/50.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
ICC/IF
1/100 - 1/250. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
|
IP
1/50. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB). -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the GST superfamily. Kappa family. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 373156 Human
- Omim: 602321 Human
- SwissProt: Q9Y2Q3 Human
- Unigene: 390667 Human
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Alternative names
- EC 2.5.1.18 antibody
- Glutathione S Transferase kappa 1 antibody
- Glutathione S transferase subunit 13 antibody
see all
Images
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Western blot - Anti-GSTK1 antibody [EP1938Y] (ab52759)All lanes : Anti-GSTK1 antibody [EP1938Y] (ab52759) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : GSTK1 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab52759 observed at 27 kDa. Red - loading control ab8245 observed at 36 kDa.
ab52759 Anti-GSTK1 antibody [EP1938Y] was shown to specifically react with GSTK1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266112 (knockout cell lysate ab257461) was used. Wild-type and GSTK1 knockout samples were subjected to SDS-PAGE. ab52759 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-GSTK1 antibody [EP1938Y] (ab52759)](https://www.abcam.com/ps/products/52/ab52759/Images/ab52759-4-anti-gstk1-antibody-ep1938y-immunocytochemistry-a549-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GSTK1 antibody [EP1938Y] (ab52759)Immunocytochemistry analysis of A549 (human lung carcinoma epithelial cell) labeling GSTK1 with purified ab52759 at 1/100 dilution (10 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.13 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTK1 antibody [EP1938Y] (ab52759)](https://www.abcam.com/ps/products/52/ab52759/Images/ab52759_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTK1 antibody [EP1938Y] (ab52759)Immunohistochemical staining of paraffin-embedded human liver using ab52759 at a 1:100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-GSTK1 antibody [EP1938Y] (ab52759)](https://www.abcam.com/ps/products/52/ab52759/Images/ab52759-6-anti-gstk1-antibody-ep1938y-immunoprecipitation-heart-human.jpg)
Immunoprecipitation - Anti-GSTK1 antibody [EP1938Y] (ab52759)Purified ab52759 at 1/50 dilution (2µg) immunoprecipitating GSTK1 in Human heart lysate.
Lane 1 (input): Human heart lysate 10µg
Lane 2 (+): ab52759 + Human heart lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52759 in Human heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 25 kDa -
![Western blot - Anti-GSTK1 antibody [EP1938Y] (ab52759)](https://www.abcam.com/ps/products/52/ab52759/Images/ab52759_1.jpg)
Western blot - Anti-GSTK1 antibody [EP1938Y] (ab52759)Anti-GSTK1 antibody [EP1938Y] (ab52759) at 1/5000 dilution + Fetal liver lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa -
![Anti-GSTK1 antibody [EP1938Y] (ab52759)](https://www.abcam.com/ps/products/52/ab52759/Images/ab52759-5-benefits-of-recombinant-antibodies.png)
Anti-GSTK1 antibody [EP1938Y] (ab52759)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab52759 has been referenced in 2 publications.
- Crosby LM et al. Toxicological and chemical studies of wastewater from hydraulic fracture and conventional shale gas wells. Environ Toxicol Chem 37:2098-2111 (2018). PubMed: 29630745
- Campos-Pereira FD et al. Early cytotoxic and genotoxic effects of atrazine on Wistar rat liver: a morphological, immunohistochemical, biochemical, and molecular study. Ecotoxicol Environ Saf 78:170-7 (2012). IHC-FoFr . PubMed: 22153302