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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-GSTK1 antibody [EP1938Y] - BSA and Azide free (ab247331)

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Western blot - Anti-GSTK1 antibody [EP1938Y] - BSA and Azide free (ab247331)
  • Immunocytochemistry/ Immunofluorescence - Anti-GSTK1 antibody [EP1938Y] - BSA and Azide free (ab247331)
  • Immunoprecipitation - Anti-GSTK1 antibody [EP1938Y] - BSA and Azide free (ab247331)
  • Anti-GSTK1 antibody [EP1938Y] - BSA and Azide free (ab247331)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1938Y] to GSTK1 - BSA and Azide free
  • Suitable for: IHC-P, WB, IP, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-GSTK1 antibody [EP1938Y] - BSA and Azide free
    See all GSTK1 primary antibodies

  • Description

    Rabbit monoclonal [EP1938Y] to GSTK1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody reacts with GSTK1.

  • Tested applications

    Suitable for: IHC-P, WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, HeLa and Daudi cell lysates. ICC/IF: A549 cells IP: Human heart lysate.

  • General notes

    ab247331 is the carrier-free version of ab52759.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB).

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the GST superfamily. Kappa family.

  • Cellular localization

    Peroxisome.
  • Information by UniProt

  • Database links

    • Alternative names

      • EC 2.5.1.18 antibody
      • Glutathione S Transferase kappa 1 antibody
      • Glutathione S transferase subunit 13 antibody
      • Glutathione S-transferase k1 antibody
      • Glutathione S-transferase kappa 1 antibody
      • Glutathione S-transferase subunit 13 antibody
      • Glutathione S-transferase subunit 13 homolog antibody
      • GST 13 13 antibody
      • GST 13-13 antibody
      • GST antibody
      • GST class kappa antibody
      • GST class-kappa antibody
      • GST13 antibody
      • GST13-13 antibody
      • GSTK1 1 antibody
      • Gstk1 antibody
      • GSTK1-1 antibody
      • GSTK1_HUMAN antibody
      • hGSTK1 antibody
      see all

    Images

    • Western blot - Anti-GSTK1 antibody [EP1938Y] - BSA and Azide free (ab247331)
      Western blot - Anti-GSTK1 antibody [EP1938Y] - BSA and Azide free (ab247331)
      All lanes : Anti-GSTK1 antibody [EP1938Y] (ab52759) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : GSTK1 knockout HEK293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Daudi cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 25 kDa
      Observed band size: 27 kDa why is the actual band size different from the predicted?



      This data was developed using the same antibody clone in a different buffer formulation (ab52759).

      Lanes 1-4: Merged signal (red and green). Green - ab52759 observed at 27 kDa. Red - loading control ab8245 observed at 36 kDa. 

       ab52759 Anti-GSTK1 antibody [EP1938Y] was shown to specifically react with GSTK1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266112 (knockout cell lysate ab257461) was used.  Wild-type and GSTK1 knockout samples were subjected to SDS-PAGE.  ab52759 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-GSTK1 antibody [EP1938Y] - BSA and Azide free (ab247331)
      Immunocytochemistry/ Immunofluorescence - Anti-GSTK1 antibody [EP1938Y] - BSA and Azide free (ab247331)
      This data was developed using the same antibody clone in a different buffer formulation (ab52759).
      Immunocytochemistry analysis of A549 (human lung carcinoma epithelial cell) labeling GSTK1 with purified ab52759 at 1/100 dilution (10 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.13 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
      Negative control: PBS instead of the primary antibody.
    • Immunoprecipitation - Anti-GSTK1 antibody [EP1938Y] - BSA and Azide free (ab247331)
      Immunoprecipitation - Anti-GSTK1 antibody [EP1938Y] - BSA and Azide free (ab247331)

      This data was developed using ab52759, the same antibody clone in a different buffer formulation.
      Purified ab52759 at 1/50 dilution (2µg) immunoprecipitating GSTK1 in Human heart lysate.
      Lane 1 (input): Human heart lysate 10µg
      Lane 2 (+): ab52759 + Human heart lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52759 in Human heart lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 25 kDa

    • Anti-GSTK1 antibody [EP1938Y] - BSA and Azide free (ab247331)
      Anti-GSTK1 antibody [EP1938Y] - BSA and Azide free (ab247331)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab247331? Please let us know so that we can cite the reference in this datasheet.

    ab247331 has not yet been referenced specifically in any publications.

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