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RecombinantRabMAb

Recombinant Anti-GUCY1B3 antibody [EPR8822] (ab154841)

Reviews (2) Submit a question References (9)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
  • Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
  • Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
  • Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
  • Anti-GUCY1B3 antibody [EPR8822] (ab154841)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8822] to GUCY1B3
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-GUCY1B3 antibody [EPR8822]
    See all GUCY1B3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8822] to GUCY1B3

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GUCY1B3 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Recombinant Human GUCY1B3 protein (ab152446) can be used as a positive control in WB. Human placenta lysate; Human fetal brain and fetal lung lysates; Human pancreas tissue; Human thyroid gland carcinoma tissue; Mouse brain; Rat brain; Rat spleen; Human kidney.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR8822

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
IHC-P
1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use at 1/100 -1/250. 
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
IHC-P
1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use at 1/100 -1/250. 

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling GUCY1B3 with Purified ab154841 at 1:400 dilution (2.54 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    All lanes : Anti-GUCY1B3 antibody [EPR8822] (ab154841) at 1/5000 dilution (purified)

    Lane 1 : Human fetal lung lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat brain lysates
    Lane 4 : Rat spleen lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 71 kDa



    Blocking and diluting buffer : 5% NFDM/TBST
  • Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    All lanes : Anti-GUCY1B3 antibody [EPR8822] (ab154841) at 1/1000 dilution (unpurified)

    Lane 1 : Human placenta lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal lung lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 71 kDa

  • Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Western blot - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    All lanes : Anti-GUCY1B3 antibody [EPR8822] (ab154841) at 1/2000 dilution (unpurified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat heart tissue lysate
    Lane 7 : Rat kidney tissue lysate
    Lane 8 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 71 kDa
    Observed band size: 65 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling GUCY1B3 using unpurified ab154841 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thyroid gland carcinoma tissue, labeling GUCY1B3 using unpurified ab154841 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)

    Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using unpurified ab154841 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)

    Immunohistochemical analysis of paraffin embedded Human heart tissue using unpurified ab154841 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GUCY1B3 antibody [EPR8822] (ab154841)

    Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using unpurified ab154841 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-GUCY1B3 antibody [EPR8822] (ab154841)
    Anti-GUCY1B3 antibody [EPR8822] (ab154841)

References (9)

Publishing research using ab154841? Please let us know so that we can cite the reference in this datasheet.

ab154841 has been referenced in 9 publications.

  • Galley JC  et al. Angiotensin II augments renal vascular smooth muscle soluble GC expression via an AT1 receptor-forkhead box subclass O transcription factor signalling axis. Br J Pharmacol 179:2490-2504 (2022). PubMed: 33963547
  • Schenk MW  et al. Soluble guanylate cyclase signalling mediates etoposide resistance in progressing small cell lung cancer. Nat Commun 12:6652 (2021). PubMed: 34789728
  • Brescia M  et al. Multi-Compartment, Early Disruption of cGMP and cAMP Signalling in Cardiac Myocytes from the mdx Model of Duchenne Muscular Dystrophy. Int J Mol Sci 21:N/A (2020). PubMed: 32992747
  • Claveria-Cabello A  et al. Dual Pharmacological Targeting of HDACs and PDE5 Inhibits Liver Disease Progression in a Mouse Model of Biliary Inflammation and Fibrosis. Cancers (Basel) 12:N/A (2020). PubMed: 33322158
  • Mukohda M  et al. RhoBTB1 protects against hypertension and arterial stiffness by restraining phosphodiesterase 5 activity. J Clin Invest 130:2318-2332 (2019). PubMed: 30896450
  • Agbor LN  et al. Conditional deletion of smooth muscle Cullin-3 causes severe progressive hypertension. JCI Insight 5:N/A (2019). PubMed: 31184598
  • Zhan R  et al. Nitric oxide induces epidermal stem cell de-adhesion by targeting integrin ß1 and Talin via the cGMP signalling pathway. Nitric Oxide 78:1-10 (2018). WB . PubMed: 29698689
  • Wales JA  et al. Discovery of stimulator binding to a conserved pocket in the heme domain of soluble guanylyl cyclase. J Biol Chem 293:1850-1864 (2018). PubMed: 29222330
  • Prasanna G  et al. A Novel Selective Soluble Guanylate Cyclase Activator, MGV354, Lowers Intraocular Pressure in Preclinical Models, Following Topical Ocular Dosing. Invest Ophthalmol Vis Sci 59:1704-1716 (2018). PubMed: 29610853

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry free floating abreview for Anti-Guanylyl Cyclase beta 1 antibody [EPR8822]

 Good
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brainstem, cochlear nuclei)
Specification
brainstem, cochlear nuclei
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Lin Lin

Verified customer

Submitted Mar 31 2017

Western blot abreview for Anti-Guanylyl Cyclase beta 1 antibody [EPR8822]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (lung, liver)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
lung, liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 07 2016

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