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  1. Link

    products/primary-antibodies/ha-tag-antibody-chip-grade-ab9110.pdf

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Anti-HA tag antibody - ChIP Grade (ab9110)

  • Datasheet
  • SDS
Reviews (58)Q&A (12)References (1070)

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Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)
  • Immunoprecipitation - Anti-HA tag antibody - ChIP Grade (ab9110)
  • Western blot - Anti-HA tag antibody - ChIP Grade (ab9110)
  • ChIP - Anti-HA tag antibody - ChIP Grade (ab9110)
  • ChIP - Anti-HA tag antibody - ChIP Grade (ab9110)
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)

Key features and details

  • Rabbit polyclonal to HA tag - ChIP Grade
  • Suitable for: ChIP/Chip, IP, ELISA, WB, ICC/IF, Flow Cyt, ChIP
  • Reacts with: Species independent
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-HA tag antibody [EPR22819-101] (ab236632)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-HA tag antibody - ChIP Grade
    See all HA tag primary antibodies
  • Description

    Rabbit polyclonal to HA tag - ChIP Grade
  • Host species

    Rabbit
  • Specificity

    ELISA: The anti HA diluted 1:70.000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification. HRP conjugated Goat anti rabbit IgG was used and TMB was the substrate.
  • Tested applications

    Suitable for: ChIP/Chip, IP, ELISA, WB, ICC/IF, Flow Cyt, ChIPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to Influenza A HA tag conjugated to keyhole limpet haemocyanin. Influenza hemagglutinin-HA-epitope

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: 293FT cells transfected with 15kDa HA tagged Vpr (an HIV1 accessory protein). IP: Nuclear lysate of HEK-293T cells transiently expressing HA-tagged protein. ICC/IF: U-2 cells. Mouse olineu cells. ChIP: Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • HA Tag
    • Signal Transduction
    • Antibodies
    • ha tag

Associated products

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • HA tag peptide (ab13835)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP/Chip (1)
Use at an assay dependent concentration.
IP (12)
Use at an assay dependent concentration.
ELISA
1/200 - 1/500.
WB (30)
1/4000 - 1/10000.
ICC/IF (8)
Use a concentration of 1 - 4 µg/ml.
Flow Cyt (1)
Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ChIP (2)
Use 3 µg for 25 µg of chromatin.
Notes
ChIP/Chip
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ELISA
1/200 - 1/500.
WB
1/4000 - 1/10000.
ICC/IF
Use a concentration of 1 - 4 µg/ml.
Flow Cyt
Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ChIP
Use 3 µg for 25 µg of chromatin.

Target

  • Relevance

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)

    Immunofluorescent analysis of 4% paraformaldehyde (PFA) fixed, permeablised with 0.1% Triton X-100 CHO cells transfected with GFP-HA constructs (CHO-GFP-HA) labelling HA tag with ab9110 at 5µg/ml, followed by Donkey Anti-Rabbit IgG(H&L), Alexa 594 conjugated antibody at 2.5µg/ml (Red). Nucleus was counterstained with DAPI (Blue). Parallel staining in parental CHO cell line as negative control.

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)This image is courtesy of an Abreview

    ab9110 staining HA-tagged proteins in HeLa cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 3% serum for 30 minutes at 37°C. Samples were incubated with primary antibody (2 µg/ml) in 1x PBS for 1 hour at 37°C. An Alexa Fluor® 488-conjugated Goat polyclonal to rabbit was used as secondary antibody. 

    See Abreview

  • Immunoprecipitation - Anti-HA tag antibody - ChIP Grade (ab9110)
    Immunoprecipitation - Anti-HA tag antibody - ChIP Grade (ab9110)This image is courtesy of an anonymous Abreview

    ab9110 was diluted to 4 µg/mg lysate and incubated with a nuclear lysate of HEK293T cells transiently expressing HA-tagged protein and a Protein A matrix for 2 hours a 23°C to achieve immunoprecipitation.  1000 µg of lysate was present in the input. 

    A HRP-conjugated anti-rabbit HA monoclonal antibody diluted 1/1000 was used for the Western Blot step.

    See Abreview

  • Western blot - Anti-HA tag antibody - ChIP Grade (ab9110)
    Western blot - Anti-HA tag antibody - ChIP Grade (ab9110)
    All lanes : Anti-HA tag antibody - ChIP Grade (ab9110) at 1/4000 dilution

    Lane 1 : 15ug untransfected wcl lysate
    Lane 2 : 293FT cells transfected with 15kDa HA tagged Vpr (an HIV1 accessory protein)

    Secondary
    All lanes : HRP conjugated Goat anti-Rabbit

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 5 seconds


    This image is courtesy of an Abreview

    See Abreview

  • ChIP - Anti-HA tag antibody - ChIP Grade (ab9110)
    ChIP - Anti-HA tag antibody - ChIP Grade (ab9110)

    Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 20µl of Protein A/G sepharose beads, and 3µg of ab9110 (anti-HA, light blue) or, 3µg of ab18337 (anti-Boris, dark blue). A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).

  • ChIP - Anti-HA tag antibody - ChIP Grade (ab9110)
    ChIP - Anti-HA tag antibody - ChIP Grade (ab9110)This image is courtesy of an Abreview submitted by Mr. Dan Stummer

    Chromatin was prepared according to the X-ChIP protocol. Mouse embryonic stem whole cell lysate treated with disuccinimidyl glutarate (cross-linking agent). ChIP was performed using ab9110 at 1/200 dilution for 16 hours at 4°C in RIPA diluent. The bound DNA was quantitated by real-time PCR. Negative control: The parent cell line. Positive control: A cell line, which stably express an HA-tagged RARgamma protein.

     
     

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)Seitz, K. et al Send to PLoS One. 2014 Jan 29;9(1):e87132. doi: 10.1371/journal.pone.0087132. eCollection 2014 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Arrb2 depends on Gβγ to induce membrane translocation of PKCα

    Xenopus embryos were injected with 500 pg pkcα-gfp RNA and co-injected as indicated above the images. Animal Caps were prepared at stage 10 and immunostained as indicated. Nuclei were stained with Hoechst 33258 (blue). Images show representative results from at least two independent experiments with a minimum of six Animal Caps per experiment. Scale bars: 50 µm.

    The inhibitory effect of Arrb2 MO1 (E) on PKCα-GFP membrane translocation was rescued by (F) co-injection of HA-Gβ and HA-Gγ mRNA (anti-HA (red): F′, merge: F").

    HA was detected with ab9110.

    (After Figure 2 of Seitz et al)

     

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)
    This image was kindly supplied as part of the review submitted by Kasper Fugger. Immunofluorescence staining of U-2 cells expressing HA-tagged protein with ab9110.
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody - ChIP Grade (ab9110)

    ab 9110 at a 1/200 dilution staining the mouse olineu cell line (oligodendrocyte precursor cell) by immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy5 conjugated goat anti-rabbit antibody.

    This image is courtesy of an Abreview submitted by Katarina Trajkovic on 15 March 2006

    See Abreview

Protocols

  • ChIP protocols
  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1070)

Publishing research using ab9110? Please let us know so that we can cite the reference in this datasheet.

ab9110 has been referenced in 1070 publications.

  • Stanković D  et al. Drosophila pVALIUM10 TRiP RNAi lines cause undesired silencing of Gateway-based transgenes. Life Sci Alliance 6:N/A (2023). PubMed: 36446522
  • Derrien AC  et al. Functional characterization of 5p15.33 risk locus in uveal melanoma reveals rs452384 as a functional variant and NKX2.4 as an allele-specific interactor. Am J Hum Genet 109:2196-2209 (2022). PubMed: 36459980
  • Ibrahim Z  et al. Structural insights into p300 regulation and acetylation-dependent genome organisation. Nat Commun 13:7759 (2022). PubMed: 36522330
  • Sakaguchi C  et al. Identification and characterization of novel proteins associated with CHD4. Genes Cells 27:61-71 (2022). PubMed: 34897913
  • Wang W  et al. Inhibitory effect of CC chemokine ligand 23 (CCL23)/ transcription factor activating enhancer binding protein 4 (TFAP4) on cell proliferation, invasion and angiogenesis in hepatocellular carcinoma. Bioengineered 13:1626-1636 (2022). PubMed: 35001801
View all Publications for this product

Customer reviews and Q&As

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1-10 of 70 Abreviews or Q&A

Western blot abreview for Anti-HA tag antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (megakaroblastic cells)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10)
Loading amount
30 µg
Specification
megakaroblastic cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 31 2022

Immunocytochemistry abreview for Anti-HA tag antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (hek293)
Permeabilization
No
Specification
hek293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Robert Kupp

Verified customer

Submitted Jan 07 2022

Western blot abreview for Anti-HA tag antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10)
Loading amount
30 µg
Treatment
Transfected with HA-tagged protein
Specification
Liver
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 06 2020

Western blot abreview for Anti-HA tag antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
20 µg
Specification
293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 14 2020

Western blot abreview for Anti-HA tag antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
0.2 µg
Specification
HeLa
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 29 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-HA tag antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.1% saponin
Specification
HeLa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 29 2019

Western blot abreview for Anti-HA tag antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (fibroblast)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
fibroblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 07 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-HA tag antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH-3T3)
Permeabilization
Yes - 0.5% tritonX-100
Specification
NIH-3T3
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Zheng Wang

Verified customer

Submitted Nov 14 2018

Immunoprecipitation abreview for Anti-HA tag antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (Co-IP from mouse NSCs)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
Co-IP from mouse NSCs
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 01 2018

Western blot abreview for Anti-HA tag antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse neural stem cell)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
mouse neural stem cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 12 2018

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