Recombinant Anti-HA tag antibody [EPR4095] (ab182009)
![Western blot - Anti-HA tag antibody [EPR4095] (ab182009)](https://www.abcam.com/ps/products/182/ab182009/Images/ab182009-339735-ab182009WB1.jpg "Western blot - Anti-HA tag antibody [EPR4095] (ab182009)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4095] to HA tag
- Suitable for: WB
- Reacts with: Species independent
Related conjugates and formulations
Overview
-
Product name
Anti-HA tag antibody [EPR4095]
See all HA tag primary antibodies -
Description
Rabbit monoclonal [EPR4095] to HA tag -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Multi-tag fusion protein. HA-tagged Human Glutaminase C overexpression lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4095 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. |
Target
-
Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Images
-
Western blot - Anti-HA tag antibody [EPR4095] (ab182009)Anti-HA tag antibody [EPR4095] (ab182009) at 1/10000 dilution (Purified) + HA-tagged Human Glutaminase C overexpression lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 57 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-HA tag antibody [EPR4095] (ab182009)](https://www.abcam.com/ps/products/182/ab182009/Images/ab182009-215356-anti-ha-tag-antibody-epr4095-western-blot.jpg)
Western blot - Anti-HA tag antibody [EPR4095] (ab182009)Anti-HA tag antibody [EPR4095] (ab182009) at 1/5000 dilution + multi-tag fusion protein, 1 ng
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutionBlocking/ Dilution buffer: 5% NFDM /TBST.
-
![Anti-HA tag antibody [EPR4095] (ab182009)](https://www.abcam.com/ps/products/182/ab182009/Images/ab182009-3-benefits-of-recombinant-antibodies.png)
Anti-HA tag antibody [EPR4095] (ab182009)
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (5)
ab182009 has been referenced in 5 publications.
- Lu Y et al. Differential Degradation of TRA2A and PYCR2 Mediated by Ubiquitin E3 Ligase E4B. Front Cell Dev Biol 10:833396 (2022). PubMed: 35669517
- Guo Q et al. ZEB2, interacting with MDM2, contributes to the dysfuntion of brain microvascular endothelial cells and brain injury after intracerebral hemorrhage. Cell Cycle 20:1692-1707 (2021). PubMed: 34334113
- Barz S et al. Atg1 kinase regulates autophagosome-vacuole fusion by controlling SNARE bundling. EMBO Rep 21:e51869 (2020). PubMed: 33274589
- Hollenstein DM et al. Vac8 spatially confines autophagosome formation at the vacuole in S. cerevisiae. J Cell Sci 132:N/A (2019). PubMed: 31649143
- Driesbaugh KH et al. Proteolytic activation of the protease-activated receptor (PAR)-2 by the glycosylphosphatidylinositol-anchored serine protease testisin. J Biol Chem 290:3529-41 (2015). Human . PubMed: 25519908