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  1. Link

    products/primary-antibodies/ha-tag-antibody-hac5-ab18181.pdf

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Tags & Cell Markers Epitope Tags HA Tag
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Anti-HA tag antibody [HA.C5] (ab18181)

  • Datasheet
  • SDS
Reviews (19)Q&A (2)References (192)

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Western blot - Anti-HA tag antibody [HA.C5] (ab18181)
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)
  • Western blot - Anti-HA tag antibody [HA.C5] (ab18181)
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)
  • Western blot - Anti-HA tag antibody [HA.C5] (ab18181)

Key features and details

  • Mouse monoclonal [HA.C5] to HA tag
  • Suitable for: WB, ICC/IF
  • Reacts with: Species independent
  • Isotype: IgG3

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Overview

  • Product name

    Anti-HA tag antibody [HA.C5]
    See all HA tag primary antibodies
  • Description

    Mouse monoclonal [HA.C5] to HA tag
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide from influenza hemagglutinin epitope:

    YPYDVPDYA

    conjugated to KLH.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from TCS
  • Clonality

    Monoclonal
  • Clone number

    HA.C5
  • Isotype

    IgG3
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • HA Tag

Associated products

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG3, kappa monoclonal [MG3-35] - Isotype Control (ab18394)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • HA tag peptide (ab13835)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (12)
1/1000.
ICC/IF (4)
Use at an assay dependent concentration.
Notes
WB
1/1000.
ICC/IF
Use at an assay dependent concentration.

Target

  • Relevance

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • Western blot - Anti-HA tag antibody [HA.C5] (ab18181)
    Western blot - Anti-HA tag antibody [HA.C5] (ab18181)This image is courtesy of an anonymous Abreview
    All lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/1000 dilution

    Lanes 1-2 : HEK293 whole cell lysate - transfected
    Lane 3 : HEK293 whole cell lysate - untransfected

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : IRDye® 800CW Goat anti-mouse IgG polyclonal at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size: 85 kDa why is the actual band size different from the predicted?


    Exposure time: 5 minutes

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)This image is courtesy of an Abreview submitted by Harendra Chahar
    ab18181 staining HA tag (green) in HeLa cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

    See Abreview

  • Western blot - Anti-HA tag antibody [HA.C5] (ab18181)
    Western blot - Anti-HA tag antibody [HA.C5] (ab18181)
    Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1). Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1).
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)
    Immunofluorescence using ab18181 staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel).
  • Western blot - Anti-HA tag antibody [HA.C5] (ab18181)
    Western blot - Anti-HA tag antibody [HA.C5] (ab18181)This image is courtesy of an anonymous Abreview
    All lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/2000 dilution

    Lane 1 : WCE from cell line transfected for HA-tagged protein
    Lane 2 : WCE from a cell line transfected with empty vector

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP conjugated Goat anti-mouse IgG (H+L)

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds


    Incubation with the primary antibody was carried out at 4°C overnight, whilst the secondary antibody was incubated for 1 hour at room temperature.

    See Abreview

Protocols

  • ChIP protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (192)

Publishing research using ab18181? Please let us know so that we can cite the reference in this datasheet.

ab18181 has been referenced in 192 publications.

  • Dong H  et al. ELONGATED HYPOCOTYL 5 mediates blue light-induced starch degradation in tomato. J Exp Bot 72:2627-2641 (2021). PubMed: 33377142
  • Shen M  et al. Structural basis for the multi-activity factor Rad5 in replication stress tolerance. Nat Commun 12:321 (2021). PubMed: 33436623
  • Malik AU  et al. Deciphering the LRRK code: LRRK1 and LRRK2 phosphorylate distinct Rab proteins and are regulated by diverse mechanisms. Biochem J 478:553-578 (2021). PubMed: 33459343
  • Eastwood EL  et al. Dimerisation of the PICTS complex via LC8/Cut-up drives co-transcriptional transposon silencing in Drosophila. Elife 10:N/A (2021). PubMed: 33538693
  • Meng J  et al. PFKFB4 promotes lung adenocarcinoma progression via phosphorylating and activating transcriptional coactivator SRC-2. BMC Pulm Med 21:60 (2021). PubMed: 33593309
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 21 Abreviews or Q&A

Western blot abreview for Anti-HA tag antibody [HA.C5]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (4-20)
Loading amount
10 µg
Treatment
transfected with HA-tagged protein
Specification
HEK293
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 02 2021

Western blot abreview for Anti-HA tag antibody [HA.C5]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (10% NuPAGE Gel Bis-Tris)
Loading amount
10 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 14 2019

Immunohistochemistry (Frozen sections) abreview for Anti-HA tag antibody [HA.C5]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (frozen liver sections)
Permeabilization
Yes - Triton X 0.3% - 10 min
Specification
frozen liver sections
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 30 2016

Western blot abreview for Anti-HA tag antibody [HA.C5]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Gel Running Conditions
Non-reduced Denaturing (10% SDS)
Loading amount
80 µg
Specification
293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Qingyuan Yang

Verified customer

Submitted Dec 30 2015

Western blot abreview for Anti-HA tag antibody [HA.C5]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
1 µg
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Sample
Aedes aegypti (Mosquito) Recombinant protein (aedes midgut protein made in E.coli)
Specification
aedes midgut protein made in E.coli
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 31 2015

Western blot abreview for Anti-HA tag antibody [HA.C5]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Human Cell lysate - whole cell (Renal cancer cell line)
Specification
Renal cancer cell line
Treatment
Doxicycline induction of Protein_HA tag
Blocking step
Li-cor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 28 2014

Immunoprecipitation abreview for Anti-HA tag antibody [HA.C5]

Average
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Treatment
untransfected/ transfected ha-tagged protein
Total protein in input
100 µg
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 27 2013

Western blot abreview for Anti-HA tag antibody [HA.C5]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
Odyssey block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 25 2013

Immunoprecipitation abreview for Anti-HA tag antibody [HA.C5]

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (CHO cells)
Total protein in input
500 µg
Specification
CHO cells
Immuno-precipitation step
Protein A/G
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 26 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-HA tag antibody [HA.C5]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2)
Specification
HepG2
Fixative
Paraformaldehyde
Permeabilization
Yes - Saponin 0.1%
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 19°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 12 2013

1-10 of 21 Abreviews or Q&A

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