Anti-HA tag antibody [HA.C5] (ab18181)
![Western blot - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.com/ps/products/18/ab18181/Images/ab18181-212146-anti-ha-tag-antibody-hac5-western-blot.jpg "Western blot - Anti-HA tag antibody [HA.C5] (ab18181)")
Key features and details
- Mouse monoclonal [HA.C5] to HA tag
- Suitable for: WB, ICC/IF
- Reacts with: Species independent
- Isotype: IgG3
Overview
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Product name
Anti-HA tag antibody [HA.C5]
See all HA tag primary antibodies -
Description
Mouse monoclonal [HA.C5] to HA tag -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide from influenza hemagglutinin epitope:
YPYDVPDYA
conjugated to KLH. -
General notes
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
HA.C5 -
Isotype
IgG3 -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (12) |
1/1000.
|
| ICC/IF | (4) |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
1/1000. |
|
ICC/IF
Use at an assay dependent concentration. |
Target
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Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Images
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Western blot - Anti-HA tag antibody [HA.C5] (ab18181)This image is courtesy of an anonymous AbreviewAll lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/1000 dilution
Lanes 1-2 : HEK293 whole cell lysate - transfected
Lane 3 : HEK293 whole cell lysate - untransfected
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : IRDye® 800CW Goat anti-mouse IgG polyclonal at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.com/ps/products/18/ab18181/Images/ab18181-26826-anti-ha-tag-antibody-hac5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)This image is courtesy of an Abreview submitted by Harendra Chaharab18181 staining HA tag (green) in HeLa cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue). -
![Western blot - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.com/ps/products/18/ab18181/Images/ab18181-26829-anti-ha-tag-antibody-hac5-western-blot.jpg)
Western blot - Anti-HA tag antibody [HA.C5] (ab18181)Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1). Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1). -
![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.com/ps/products/18/ab18181/Images/ab18181-26828-anti-ha-tag-antibody-hac5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)Immunofluorescence using ab18181 staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel). -
![Western blot - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.com/ps/products/18/ab18181/Images/ab18181-26827-anti-ha-tag-antibody-hac5-western-blot.jpg)
Western blot - Anti-HA tag antibody [HA.C5] (ab18181)This image is courtesy of an anonymous AbreviewAll lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/2000 dilution
Lane 1 : WCE from cell line transfected for HA-tagged protein
Lane 2 : WCE from a cell line transfected with empty vector
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-mouse IgG (H+L)
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
Incubation with the primary antibody was carried out at 4°C overnight, whilst the secondary antibody was incubated for 1 hour at room temperature.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (192)
ab18181 has been referenced in 192 publications.
- Dong H et al. ELONGATED HYPOCOTYL 5 mediates blue light-induced starch degradation in tomato. J Exp Bot 72:2627-2641 (2021). PubMed: 33377142
- Shen M et al. Structural basis for the multi-activity factor Rad5 in replication stress tolerance. Nat Commun 12:321 (2021). PubMed: 33436623
- Malik AU et al. Deciphering the LRRK code: LRRK1 and LRRK2 phosphorylate distinct Rab proteins and are regulated by diverse mechanisms. Biochem J 478:553-578 (2021). PubMed: 33459343
- Eastwood EL et al. Dimerisation of the PICTS complex via LC8/Cut-up drives co-transcriptional transposon silencing in Drosophila. Elife 10:N/A (2021). PubMed: 33538693
- Meng J et al. PFKFB4 promotes lung adenocarcinoma progression via phosphorylating and activating transcriptional coactivator SRC-2. BMC Pulm Med 21:60 (2021). PubMed: 33593309