Recombinant Anti-HA tag antibody [HL1603] (ab308398)
![Western blot - Anti-HA tag antibody [HL1603] (ab308398)](https://www.abcam.com/ps/products/308/ab308398/Images/ab308398-9-AntiHA-tag-antibody-HL1603-WB-HEK293t-transfected-cells.jpg "Western blot - Anti-HA tag antibody [HL1603] (ab308398)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HL1603] to HA tag - BSA and Azide free
- Suitable for: IHC-P, WB, Indirect ELISA, ICC/IF
Overview
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Product name
Anti-HA tag antibody [HL1603]
See all HA tag primary antibodies -
Description
Rabbit monoclonal [HL1603] to HA tag - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Indirect ELISA, ICC/IFmore details -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: transfected HEK293T cells IHC-P: HA tag transfected HEK293T cell pellet Indirect ELISA: recombinant HA-tag tagged protein WB: transfected HEK293T cell lysate,
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General notes
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HL1603 -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab308398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
1/5000.
|
|
| Indirect ELISA |
Use a concentration of 1 µg/ml.
|
|
| ICC/IF |
1/500.
|
| Notes |
|---|
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/5000. |
|
Indirect ELISA
Use a concentration of 1 µg/ml. |
|
ICC/IF
1/500. |
Target
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Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Images
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Western blot - Anti-HA tag antibody [HL1603] (ab308398)All lanes : Anti-HA tag antibody [HL1603] (ab308398) at 1/5000 dilution
Lane 1 : Non-transfected (–) HEK293T cell lysates
Lane 2 : HA tagged protein transfected (+) HEK293T cell lysates
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody -
![Indirect ELISA - Anti-HA tag antibody [HL1603] (ab308398)](https://www.abcam.com/ps/products/308/ab308398/Images/ab308398-8-AntiHA-tag-antibody-HL1603-indirect-ELISA--purified-protein.jpg)
Indirect ELISA - Anti-HA tag antibody [HL1603] (ab308398)Indirect ELISA analysis was performed by coating the plate with recombinant HA-tag tagged protein (1000-15.63 ng/mL). Coated protein was probed with HA tag antibody [HL1603] (ab308398) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (1/10000) was used to detect the bound primary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [HL1603] (ab308398)](https://www.abcam.com/ps/products/308/ab308398/Images/ab308398-7-AntiHA-tag-antibody-HL1603-IHCP-HEK293T-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [HL1603] (ab308398)Immunohistochemical analysis of mock and HA tag - transfected HEK293T cell pellet labeling HA tag using ab308398 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min -
![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HL1603] (ab308398)](https://www.abcam.com/ps/products/308/ab308398/Images/ab308398-2-AntiHA-tag-antibody-HL1603-ICC-HEK293T-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HL1603] (ab308398)For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of HA tag using ab308398 at 1/500 dilution (green). Blue: Fluoroshield with DAPI. -
![Anti-HA tag antibody [HL1603] (ab308398)](https://www.abcam.com/ps/products/308/ab308398/Images/ab308398-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-HA tag antibody [HL1603] (ab308398)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab308398 has not yet been referenced specifically in any publications.
