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Anti-HADHB antibody [5C3AB7] (ab110301)

Reviews (1) Submit a question References (1)
Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)
  • Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)
  • Immunocytochemistry - Anti-HADHB antibody [5C3AB7] (ab110301)
  • Flow Cytometry - Anti-HADHB antibody [5C3AB7] (ab110301)
  • Immunoprecipitation - Anti-HADHB antibody [5C3AB7] (ab110301)

Key features and details

  • Mouse monoclonal [5C3AB7] to HADHB
  • Suitable for: IP, WB, Flow Cyt, ICC
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 HRP

Overview

  • Product name

    Anti-HADHB antibody [5C3AB7]
    See all HADHB primary antibodies

  • Description

    Mouse monoclonal [5C3AB7] to HADHB

  • Host species

    Mouse

  • Tested applications

    Suitable for: IP, WB, Flow Cyt, ICCmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2 and HLM cell lysates; HDFn and HeLa cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 51 kDa.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC
Use a concentration of 1 µg/ml.
Notes
IP
Use at an assay dependent concentration.
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 51 kDa.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC
Use a concentration of 1 µg/ml.

Target

  • Pathway

    Lipid metabolism; fatty acid beta-oxidation.

  • Involvement in disease

    Defects in HADHB are a cause of trifunctional protein deficiency (TFP deficiency) [MIM:609015]. The clinical manifestations are very variable and include hypoglycemia, cardiomyopathy and sudden death. Phenotypes with mainly hepatic and neuromyopathic involvement can also be distinguished. Biochemically, TFP deficiency is defined by the loss of all three enzyme activities of the TFP complex.

  • Sequence similarities

    Belongs to the thiolase family.

  • Cellular localization

    Mitochondrion.
  • Information by UniProt

  • Database links

    • Alternative names

      • 2 enoyl Coenzyme A (CoA) hydratase beta subunit antibody
      • 3 ketoacyl Coenzyme A (CoA) thiolase of mitochondrial trifunctional protein beta subunit antibody
      • 3 ketoacyl Coenzyme A thiolase antibody
      • 3 ketoacyl Coenzyme A thiolase of mitochondrial trifunctional protein beta subunit antibody
      • 3-ketoacyl-CoA thiolase antibody
      • Acetyl CoA acyltransferase antibody
      • Acetyl-CoA acyltransferase antibody
      • Beta ketothiolase antibody
      • Beta-ketothiolase antibody
      • ECHB antibody
      • ECHB_HUMAN antibody
      • HADH antibody
      • Hadhb antibody
      • Hydroxyacyl CoA dehydrogenase/3 ketoacyl CoA thiolase/enoyl CoA hydratase (trifunctional protein), beta subunit antibody
      • Hydroxyacyl Coenzyme A (CoA) dehydrogenase beta subunit antibody
      • Hydroxyacyl Coenzyme A dehydrogenase antibody
      • Hydroxyacyl Coenzyme A dehydrogenase beta subunit antibody
      • Hydroxyacyl Coenzyme A dehydrogenase/3 ketoacyl Coenzyme A thiolase/enoyl Coenzyme A hydratase (trifunctional protein) beta subunit antibody
      • MGC87480 antibody
      • Mitochondrial trifunctional enzyme beta subunit antibody
      • Mitochondrial trifunctional protein beta subunit antibody
      • MSTP 029 antibody
      • MSTP029 antibody
      • MTPB antibody
      • TP beta antibody
      • TP-beta antibody
      • TPbeta antibody
      • Trifunctional enzyme subunit beta antibody
      • Trifunctional enzyme subunit beta mitochondrial antibody
      • Trifunctional protein antibody
      see all

    Images

    • Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)
      Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)
      Anti-HADHB antibody [5C3AB7] (ab110301) at 0.5 µg/ml (20 ug HepG2 cell lysate)
      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 51 kDa

    • Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)
      Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)
      Anti-HADHB antibody [5C3AB7] (ab110301) at 0.5 µg/ml + HepG2 cell lysate

      Predicted band size: 51 kDa



      As a Western blot antibody, ab110301 works with HepG2 (human) cells.

    • Immunocytochemistry - Anti-HADHB antibody [5C3AB7] (ab110301)
      Immunocytochemistry - Anti-HADHB antibody [5C3AB7] (ab110301)
      Immunocytochemistry analysis using ab110301 at 1µg/ml staining HADHB in HDFn cells (paraformaldehyde fixed and Triton X-100 permeabilized).
      The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution. DAPI was used to stain the cell nuclei (blue).
    • Flow Cytometry - Anti-HADHB antibody [5C3AB7] (ab110301)
      Flow Cytometry - Anti-HADHB antibody [5C3AB7] (ab110301)
      Flow cytometric analysis using ab110301 at 1µg/ml staining HADHB in HeLa cells (blue). Isotype control antibody (red).
    • Immunoprecipitation - Anti-HADHB antibody [5C3AB7] (ab110301)
      Immunoprecipitation - Anti-HADHB antibody [5C3AB7] (ab110301)
      As an IP antibody, MS733 works with human samples.

    References (1)

    Publishing research using ab110301? Please let us know so that we can cite the reference in this datasheet.

    ab110301 has been referenced in 1 publication.

    • Li XX  et al. Nuclear Receptor Nur77 Facilitates Melanoma Cell Survival under Metabolic Stress by Protecting Fatty Acid Oxidation. Mol Cell 69:480-492.e7 (2018). PubMed: 29395065

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-HADHB antibody [5C3AB7]

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    African Green Monkey Cell lysate - whole cell (COS cells, HeLa cells (human), N2A cells (mouse),)
    Specification
    COS cells, HeLa cells (human), N2A cells (mouse),
    Blocking step
    LiCor blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 22 2014

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