Recombinant Anti-HAI-1 antibody [EPR23294-49] (ab272721)
![Western blot - Anti-HAI-1 antibody [EPR23294-49] (ab272721)](https://www.abcam.com/ps/products/272/ab272721/Images/ab272721-393108-anti-hai1-antibody-epr2329449-western-blot-human-placenta-tissue-lysate20-ug.jpg "Western blot - Anti-HAI-1 antibody [EPR23294-49] (ab272721)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23294-49] to HAI-1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-HAI-1 antibody [EPR23294-49]
See all HAI-1 primary antibodies -
Description
Rabbit monoclonal [EPR23294-49] to HAI-1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: IHC-Fr -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human and rat placenta tissue lysate. T47D whole cell lysate. Mouse large intestine, small intestine, placenta and stomach tissue lysate. ICC/IF: T47D cells. Flow Cyt (intra): T47D cells. IHC-P: Mouse testis and stomach tissue. IP: mouse placenta tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23294-49 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/600.
(0.1ug) |
|
| WB |
1/1000. Detects a band of approximately 56, 66 kDa (predicted molecular weight: 58 kDa).
(0.616 μg/ml). |
|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/50.
|
|
| IP |
1/30.
(2μg in 0.35mg lysates) |
| Notes |
|---|
|
Flow Cyt (Intra)
1/600. (0.1ug) |
|
WB
1/1000. Detects a band of approximately 56, 66 kDa (predicted molecular weight: 58 kDa). (0.616 μg/ml). |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/50. |
|
IP
1/30. (2μg in 0.35mg lysates) |
Target
-
Function
Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14). -
Sequence similarities
Contains 2 BPTI/Kunitz inhibitor domains.
Contains 1 LDL-receptor class A domain.
Contains 1 MANSC domain. -
Domain
This inhibitor contains two inhibitory domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6692 Human
- Entrez Gene: 20732 Mouse
- Entrez Gene: 311331 Rat
- Omim: 605123 Human
- SwissProt: O43278 Human
- SwissProt: Q9R097 Mouse
- Unigene: 233950 Human
- Unigene: 104955 Mouse
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Alternative names
- HAI 1 antibody
- HAI antibody
- HAI-1 antibody
see all
Images
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Western blot - Anti-HAI-1 antibody [EPR23294-49] (ab272721)All lanes : Anti-HAI-1 antibody [EPR23294-49] (ab272721) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate
Lane 3 : Daudi (human Burkitts lymphoma lymphoblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 56,66 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
Low expression cell line: Daudi (PMID:24070417).
The molecular weight observed is consistent with what has been described in the literature (PMID:11013244 ).
Exposure time: 3 minutes.
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![Immunocytochemistry/ Immunofluorescence - Anti-HAI-1 antibody [EPR23294-49] (ab272721)](https://www.abcam.com/ps/products/272/ab272721/Images/ab272721-3-anti-hai1-antibody-epr2329449-immunocytochemistry-t47d.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HAI-1 antibody [EPR23294-49] (ab272721)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized T-47D cells labelling HAI-1 with ab272721 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2µg/ml) dilution (Green). Confocal image showing membranous staining in T-47D cell line.
Negative control: Daudi (PMID: 24070417).
ab195889 anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/ml) dilution.
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![Flow Cytometry (Intracellular) - Anti-HAI-1 antibody [EPR23294-49] (ab272721)](https://www.abcam.com/ps/products/272/ab272721/Images/ab272721-4-anti-hai1-antibody-epr2329449-flow-cytometry-daudi.jpg)
Flow Cytometry (Intracellular) - Anti-HAI-1 antibody [EPR23294-49] (ab272721)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Daudi (Human Burkitt's lymphoma lymphoblast) (Left panel) / T-47D (Human ductal breast epithelial tumor epithelial cell) (Right panel) cells labelling HAI-1 with ab272721 at 1/600 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: Daudi (PMID: 24070417).
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![Western blot - Anti-HAI-1 antibody [EPR23294-49] (ab272721)](https://www.abcam.com/ps/products/272/ab272721/Images/ab272721-393109-anti-hai1-antibody-epr2329449-western-blot-mouse-large-intestinetissue-lysate20-ug.jpg)
Western blot - Anti-HAI-1 antibody [EPR23294-49] (ab272721)All lanes : Anti-HAI-1 antibody [EPR23294-49] (ab272721) at 1/1000 dilution
Lane 1 : Mouse large intestine tissue lysate
Lane 2 : Mouse small intestine tissue lysate
Lane 3 : Mouse placenta tissue lysate
Lane 4 : Mouse stomach tissue lysate
Lane 5 : Mouse liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 56,66 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression tissue: liver (PMID:21840293).
The molecular weight observed is consistent with what has been described in the literature (PMID:11013244).
Exposure time: 3 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAI-1 antibody [EPR23294-49] (ab272721)](https://www.abcam.com/ps/products/272/ab272721/Images/ab272721-2-anti-hai1-antibody-epr2329449-immunohistochemistry-mouse-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAI-1 antibody [EPR23294-49] (ab272721)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling HAI-1 with ab272721 at 1/2000 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on germ cells of mouse testis (PMID: 16740973). The section was incubated with ab272721 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunoprecipitation - Anti-HAI-1 antibody [EPR23294-49] (ab272721)](https://www.abcam.com/ps/products/272/ab272721/Images/ab272721-5-anti-hai1-antibody-epr2329449-immunoprecipitation-mouse-placenta-tissue-lysate10-ug.jpg)
Immunoprecipitation - Anti-HAI-1 antibody [EPR23294-49] (ab272721)HAI-1 was immunoprecipitated from mouse placenta tissue lysate with ab272721 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272721 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse placenta tissue lysate 10µg.
Lane 2: ab272721 IP in mouse placenta tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272721 in mouse placenta tissue lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This blot was developed using a higher sensitivity ECL substrate.
-
![Western blot - Anti-HAI-1 antibody [EPR23294-49] (ab272721)](https://www.abcam.com/ps/products/272/ab272721/Images/ab272721-393112-anti-hai1-antibody-epr2329449-western-blot-rat-placenta-tissue-lysate20-ug.jpg)
Western blot - Anti-HAI-1 antibody [EPR23294-49] (ab272721)All lanes : Anti-HAI-1 antibody [EPR23294-49] (ab272721) at 1/1000 dilution
Lane 1 : Rat placenta tissue lysate
Lane 2 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression tissue: liver (PMID:21840293).
Exposure time: 3 minutes.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAI-1 antibody [EPR23294-49] (ab272721)](https://www.abcam.com/ps/products/272/ab272721/Images/ab272721-1-anti-hai1-antibody-epr2329449-immunohistochemistry-mouse-stomach.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAI-1 antibody [EPR23294-49] (ab272721)Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling HAI-1 with ab272721 at 1/2000 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse stomach. The section was incubated with ab272721 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Anti-HAI-1 antibody [EPR23294-49] (ab272721)](https://www.abcam.com/ps/products/272/ab272721/Images/ab272721-9-benefits-of-recombinant-antibodies.png)
Anti-HAI-1 antibody [EPR23294-49] (ab272721)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab272721 has not yet been referenced specifically in any publications.