Recombinant Anti-Haptoglobin antibody [EPR22856-233] (ab256455)
![Western blot - Anti-Haptoglobin antibody [EPR22856-233] (ab256455)](https://www.abcam.com/ps/products/256/ab256455/Images/ab256455-353912-anti-haptoglobin-antibody-epr22856-233-western-blot-mouse.jpg "Western blot - Anti-Haptoglobin antibody [EPR22856-233] (ab256455)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22856-233] to Haptoglobin
- Suitable for: IHC-P, IHC-Fr, WB
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Haptoglobin antibody [EPR22856-233]
See all Haptoglobin primary antibodies -
Description
Rabbit monoclonal [EPR22856-233] to Haptoglobin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Fr, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse serum, plasma, liver and lung lysates. IHC-P: Mouse lung, liver and cerebral cortex tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22856-233 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab256455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/2000.
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| IHC-Fr |
1/500.
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| WB |
1/1000. Predicted molecular weight: 45 kDa.
|
| Notes |
|---|
|
IHC-P
1/2000. |
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IHC-Fr
1/500. |
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WB
1/1000. Predicted molecular weight: 45 kDa. |
Target
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Function
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Anhaptoglobinemia -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 15439 Mouse
- SwissProt: Q61646 Mouse
- Unigene: 26730 Mouse
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Alternative names
- Binding peptide antibody
- BP antibody
- Haptoglobin alpha chain antibody
see all
Images
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Western blot - Anti-Haptoglobin antibody [EPR22856-233] (ab256455)All lanes : Anti-Haptoglobin antibody [EPR22856-233] (ab256455) at 1/1000 dilution
Lane 1 : Mouse serum
Lane 2 : Mouse plasma
Lane 3 : Mouse liver lysate
Lane 4 : Mouse lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 36,42,45,52 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:19805376, 16543473).
Exposure time: Lanes 1-2: 3 seconds; Lanes 3-4: 103 seconds.
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![Immunohistochemistry (Frozen sections) - Anti-Haptoglobin antibody [EPR22856-233] (ab256455)](https://www.abcam.com/ps/products/256/ab256455/Images/ab256455-4-anti-haptoglobin-antibody-epr22856-233-immunohistochemistry-frozen-lung-mouse.jpg)
Immunohistochemistry (Frozen sections) - Anti-Haptoglobin antibody [EPR22856-233] (ab256455)Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Haptoglobin with ab256455 at 1/500 (1.17 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-233] (ab256455)](https://www.abcam.com/ps/products/256/ab256455/Images/ab256455-1-anti-haptoglobin-antibody-epr22856-233-immunohistochemistry-lung-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-233] (ab256455)Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Haptoglobin with ab256455 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on bronchi of mouse lung (PMID: 14697247). The section was incubated with ab256455 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-233] (ab256455)](https://www.abcam.com/ps/products/256/ab256455/Images/ab256455-2-anti-haptoglobin-antibody-epr22856-233-immunohistochemistry-liver-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-233] (ab256455)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Haptoglobin with ab256455 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse liver (PMID: 14697247). The section was incubated with ab256455 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-233] (ab256455)](https://www.abcam.com/ps/products/256/ab256455/Images/ab256455-3-anti-haptoglobin-antibody-epr22856-233-immunohistochemistry-cerebral-cortex-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-233] (ab256455)Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling Haptoglobin with ab256455 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining on mouse cerebral cortex (PMID: 14697247). The section was incubated with ab256455 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Anti-Haptoglobin antibody [EPR22856-233] (ab256455)](https://www.abcam.com/ps/products/256/ab256455/Images/ab256455-6-benefits-of-recombinant-antibodies.png)
Anti-Haptoglobin antibody [EPR22856-233] (ab256455)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab256455 has not yet been referenced specifically in any publications.