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Recombinant

Recombinant Anti-HCN1 antibody [7C3] (ab252835)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)
  • Western blot - Anti-HCN1 antibody [7C3] (ab252835)
  • Western blot - Anti-HCN1 antibody [7C3] (ab252835)
  • Anti-HCN1 antibody [7C3] (ab252835)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [7C3] to HCN1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-HCN1 antibody [7C3]
    See all HCN1 primary antibodies

  • Description

    Rat monoclonal [7C3] to HCN1

  • Host species

    Rat

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Human, mouse and rat brain tissue lysate. IHC-P: Human, rat and mouse cerebrum tissue. Mouse and rat retina tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Associated products

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab252835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.326 µg/ml. Detects a band of approximately 99, 110 kDa (predicted molecular weight: 99 kDa).

Human: 99 kDa, Mouse, Rat:110 kDa
IHC-P
Use a concentration of 0.065 mg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Use at 1:500 dilution for human and 1:5000 dilution for mouse and rat.
Notes
WB
Use a concentration of 0.326 µg/ml. Detects a band of approximately 99, 110 kDa (predicted molecular weight: 99 kDa).

Human: 99 kDa, Mouse, Rat:110 kDa
IHC-P
Use a concentration of 0.065 mg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Use at 1:500 dilution for human and 1:5000 dilution for mouse and rat.
Application notes
Is unsuitable for ICC/IF.

Target

  • Function

    Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli.

  • Tissue specificity

    Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas.

  • Sequence similarities

    Belongs to the potassium channel HCN family.
    Contains 1 cyclic nucleotide-binding domain.

  • Domain

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • BCNG-1 antibody
    • BCNG1 antibody
    • Brain cyclic nucleotide gated channel 1 antibody
    • Brain cyclic nucleotide-gated channel 1 antibody
    • HAC 2 antibody
    • HAC2 antibody
    • HCN1 antibody
    • HCN1_HUMAN antibody
    • Hyperpolarization activated cyclic nucleotide gated potassium channel 1 antibody
    • Potassium channel, voltage-gated, brain, 1 antibody
    • Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1 antibody
    • Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling HCN1 with ab252835 at 0.065, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on rat cerebrum tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)

    Immunohistochemical analysis of paraffin-embedded rat retina tissue labeling HCN1 with ab252835 at 0.065µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on rat retina tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling HCN1 with ab252835 at 0.065µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse cerebrum tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)

    Immunohistochemical analysis of paraffin-embedded mouse retina tissue labeling HCN1 with ab252835 at 0.065µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse retina tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling HCN1 with ab252835 at 0.065µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human esophagus tissue is observed. The section was incubated with ab252835 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Western blot - Anti-HCN1 antibody [7C3] (ab252835)
    Western blot - Anti-HCN1 antibody [7C3] (ab252835)
    All lanes : Anti-HCN1 antibody [7C3] (ab252835) at 0.326 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 99 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 secs.

  • Western blot - Anti-HCN1 antibody [7C3] (ab252835)
    Western blot - Anti-HCN1 antibody [7C3] (ab252835)
    All lanes : Anti-HCN1 antibody [7C3] (ab252835) at 0.326 µg/ml

    Lane 1 : Human brain tissue lysate
    Lane 2 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 99 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 5 secs.

    The negative control and the molecular weight of HCN1 observed is consistent with what has been described in the literature (PMID: 12786975).

  • Anti-HCN1 antibody [7C3] (ab252835)
    Anti-HCN1 antibody [7C3] (ab252835)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab252835? Please let us know so that we can cite the reference in this datasheet.

ab252835 has not yet been referenced specifically in any publications.

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