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Anti-HCN1 antibody [N70/28] (ab84816)

Reviews (1) Submit a question References (16)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [N70/28] (ab84816)
  • Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody [N70/28] (ab84816)
  • Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody [N70/28] (ab84816)
  • Flow Cytometry - Anti-HCN1 antibody [N70/28] (ab84816)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [N70/28] (ab84816)
  • Western blot - Anti-HCN1 antibody [N70/28] (ab84816)

Key features and details

  • Mouse monoclonal [N70/28] to HCN1
  • Suitable for: IHC-P, IHC-Fr, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-HCN1 antibody [N70/28]
    See all HCN1 primary antibodies

  • Description

    Mouse monoclonal [N70/28] to HCN1

  • Host species

    Mouse

  • Specificity

    No cross reactivity against HCN2

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Rat HCN1 aa 750-950 (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9JKB0

  • Positive control

    • WB: Rat brain lysate. IHC-P: Mouse cerebellum tissue, mouse brain tissue, human brain tissue. ICC: Human hippocampus tissue.

  • General notes

    The clone number has been updated from S70-28 to N70/28, both clone numbers name the same antibody clone.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab84816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Notes
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli.

  • Tissue specificity

    Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas.

  • Sequence similarities

    Belongs to the potassium channel HCN family.
    Contains 1 cyclic nucleotide-binding domain.

  • Domain

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • BCNG-1 antibody
    • BCNG1 antibody
    • Brain cyclic nucleotide gated channel 1 antibody
    • Brain cyclic nucleotide-gated channel 1 antibody
    • HAC 2 antibody
    • HAC2 antibody
    • HCN1 antibody
    • HCN1_HUMAN antibody
    • Hyperpolarization activated cyclic nucleotide gated potassium channel 1 antibody
    • Potassium channel, voltage-gated, brain, 1 antibody
    • Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1 antibody
    • Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [N70/28] (ab84816)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [N70/28] (ab84816)

    Immunohistochemistry analysis of 10% formalin-fixed mouse cerebellum tissue labelling HCN1 with ab84816 at 1/1000 dilution for 1 hour at room temperature, followed by biotinylated Goat Anti-Mouse antibody (brown) for 30 minutes at room temperature. Localization: Cytoplasmic staining of Purkinje cells. Counterstain: Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature. HRP/DAB detection.

  • Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody [N70/28] (ab84816)
    Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody [N70/28] (ab84816)

    Immunohistochemistry analysis of 10% formalin-fixed human frozen brain section labelling HCN1 with ab84816 at 1/1000 dilution for 1 hour at room temperature, followed by biotinylated Goat Anti-Mouse antibody (brown) for 30 minutes at room temperature. Localization: Neurons. Counterstain: Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature. HRP/DAB detection.

  • Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody [N70/28] (ab84816)
    Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody [N70/28] (ab84816)

    Immunohistochemistry analysis of 10% formalin-fixed mouse frozen brain section labelling HCN1 with ab84816 at 1/1000 dilution for 1 hour at room temperature, followed by biotinylated Goat Anti-Mouse antibody (brown) for 30 minutes at room temperature. Counterstain: Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature. HRP/DAB detection.

  • Flow Cytometry - Anti-HCN1 antibody [N70/28] (ab84816)
    Flow Cytometry - Anti-HCN1 antibody [N70/28] (ab84816)
    Overlay histogram showing SH-SY5Y cells stained with ab84816 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84816, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [N70/28] (ab84816)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [N70/28] (ab84816)

    Immunohistochemical analysis of paraffin-embedded (Bouin’s Fixative - picric acid, acetic acid and formaldehyde) human hippocampus tissue labelling HCN1 with ab84816 at 1/1000 dilution, for 1 hour at RT followed by secondary antibody Goat Anti-Mouse (FITC) at 1/50 dilution, for 1 hour at RT. Membrane and cytosolic staining on human hippocampus tissue is observed.

  • Western blot - Anti-HCN1 antibody [N70/28] (ab84816)
    Western blot - Anti-HCN1 antibody [N70/28] (ab84816)
    Anti-HCN1 antibody [N70/28] (ab84816) at 1/1000 dilution + Rat brain membrane lysates at 15 µg

    Secondary
    HRP-Sheep Anti-Mouse IgG antibody

    Predicted band size: 99 kDa
    Observed band size: 99 kDa

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (16)

Publishing research using ab84816? Please let us know so that we can cite the reference in this datasheet.

ab84816 has been referenced in 16 publications.

  • Lei X  et al. Activation of HCN channels caused by elevated cAMP levels in periaqueductal gray promotes bone cancer pain. Neurochem Int 162:105437 (2023). PubMed: 36336089
  • Lin W  et al. Downregulation of hyperpolarization-activated cyclic nucleotide-gated channels (HCN) in the hippocampus of patients with medial temporal lobe epilepsy and hippocampal sclerosis (MTLE-HS). Hippocampus 30:1112-1126 (2020). PubMed: 32543742
  • McClements ME  et al. An AAV Dual Vector Strategy Ameliorates the Stargardt Phenotype in Adult Abca4-/- Mice. Hum Gene Ther N/A:N/A (2018). PubMed: 30381971
  • Ding W  et al. Increased HCN Channel Activity in the Gasserian Ganglion Contributes to Trigeminal Neuropathic Pain. J Pain 19:626-634 (2018). PubMed: 29366880
  • He F  et al. The role of HCN channels in peristaltic dysfunction in human ureteral tuberculosis. Int Urol Nephrol 50:639-645 (2018). WB, ICC/IF . PubMed: 29460132
View all Publications for this product

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-HCN1 antibody [S70-28]

 Inconclusive
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (HL-1 cardiac cell line)
Specification
HL-1 cardiac cell line
Fixative
Methanol
Blocking step
Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 15 2010

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