Recombinant Anti-HCN2 antibody [4A6] (ab252836)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)](https://www.abcam.com/ps/products/252/ab252836/Images/ab252836-383235-AntiHCN2-antibody-4A6--ihc-rat-cerebrum.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [4A6] to HCN2
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-HCN2 antibody [4A6]
See all HCN2 primary antibodies -
Description
Rat monoclonal [4A6] to HCN2 -
Host species
Rat -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
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Positive control
- IHC-P: Human, mouse and rat cerebrum tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4A6 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 1.235 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Use at 1:200 dilution for human, 1:500 dilution for mouse and rat. |
| Notes |
|---|
|
IHC-P
Use a concentration of 1.235 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Use at 1:200 dilution for human, 1:500 dilution for mouse and rat. |
Target
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Function
Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. -
Tissue specificity
Highly expressed throughout the brain. Detected at low levels in heart. -
Sequence similarities
Belongs to the potassium channel HCN family.
Contains 1 cyclic nucleotide-binding domain. -
Domain
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 610 Human
- Entrez Gene: 15166 Mouse
- Entrez Gene: 114244 Rat
- Omim: 602781 Human
- SwissProt: Q9UL51 Human
- SwissProt: O88703 Mouse
- SwissProt: Q9JKA9 Rat
- Unigene: 124161 Human
see all -
Alternative names
- BCNG-2 antibody
- BCNG2 antibody
- Brain cyclic nucleotide gated channel 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling HCN2 with ab252836 at 1.235µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on rat cerebrum tissue is observed. The section was incubated with ab252836 for 30mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)](https://www.abcam.com/ps/products/252/ab252836/Images/ab252836-383234-AntiHCN2-antibody-4A6--ihc-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling HCN2 with ab252836 at 1.235µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse cerebrum tissue is observed. The section was incubated with ab252836 for 30mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)](https://www.abcam.com/ps/products/252/ab252836/Images/ab252836-383233-AntiHCN2-antibody-4A6--ihc-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling HCN2 with ab252836 at 1.235µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human cerebrum tissue is observed. The section was incubated with ab252836 for 30mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Anti-HCN2 antibody [4A6] (ab252836)](https://www.abcam.com/ps/products/252/ab252836/Images/ab252836-4-benefits-of-recombinant-antibodies.png)
Anti-HCN2 antibody [4A6] (ab252836)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab252836 has not yet been referenced specifically in any publications.