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Anti-HCN2 antibody [S71] (ab84817)

Reviews (4) Submit a question References (10)
Immunocytochemistry - Anti-HCN2 antibody [S71] (ab84817)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [S71] (ab84817)
  • Immunohistochemistry (Frozen sections) - Anti-HCN2 antibody [S71] (ab84817)
  • Western blot - Anti-HCN2 antibody [S71] (ab84817)
  • Western blot - Anti-HCN2 antibody [S71] (ab84817)

Key features and details

  • Mouse monoclonal [S71] to HCN2
  • Suitable for: IHC-Fr, IHC-P, ICC, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-HCN2 antibody [S71]
    See all HCN2 primary antibodies

  • Description

    Mouse monoclonal [S71] to HCN2

  • Host species

    Mouse

  • Specificity

    Does not cross react with HCN1.

  • Tested applications

    Suitable for: IHC-Fr, IHC-P, ICC, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Fusion protein corresponding to Rat HCN2 aa 750 to the C-terminus (C terminal).
    Database link: Q9JKA9

  • Positive control

    • IHC-Fr: Mouse brain tissue; IHC-P: Human hippocampus tissue; WB: Rat brain membrane and mouse brain tissue lysates; ICC: Differentiated SH-SH5Y cells.

  • General notes

    The clone number S71 has previously been called S71-37 and N71/37. These clone numbers all refer to the same antibody clone.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab84817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
1/1000.
IHC-P
1/100.
ICC
1/100.
WB (3)
1/1000. Predicted molecular weight: 95 kDa.
Notes
IHC-Fr
1/1000.
IHC-P
1/100.
ICC
1/100.
WB
1/1000. Predicted molecular weight: 95 kDa.

Target

  • Function

    Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages.

  • Tissue specificity

    Highly expressed throughout the brain. Detected at low levels in heart.

  • Sequence similarities

    Belongs to the potassium channel HCN family.
    Contains 1 cyclic nucleotide-binding domain.

  • Domain

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • BCNG-2 antibody
    • BCNG2 antibody
    • Brain cyclic nucleotide gated channel 2 antibody
    • Brain cyclic nucleotide-gated channel 2 antibody
    • HAC1 antibody
    • Hcn2 antibody
    • HCN2_HUMAN antibody
    • Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 antibody
    see all

Images

  • Immunocytochemistry - Anti-HCN2 antibody [S71] (ab84817)
    Immunocytochemistry - Anti-HCN2 antibody [S71] (ab84817)

    Immunocytochemistry analysis of differentiated SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labelling HCN2 with ab84817 at 1/100 dilution followed by secondary antibody (Alexa Fluor® 488) (green). Cells were counterstained with phalloidin (Alexa Fluor® 647) (red), and with Anti-beta III Tubulin antibody (Alexa Fluor® 555) (magenta). Nuclear DNA was labelled with Hoechst (blue).

    (A) Phalloidin

    (B) Anti-beta III Tubulin antibody

    (C) HCN2 Antibody (ab84817) and secondary antibody

    (D) Hoechst

    (E) Composite

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [S71] (ab84817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [S71] (ab84817)

    Immunohistochemistry (Bouin-fixed paraffin-embedded sections) analysis of human hippocampus tissue labelling HCN2 with ab84817 at 1/100 dilution (1 hour at room temperature). FITC Goat Anti-Mouse (green) was used as the secondary antibody at 1/50 dilution (1 hour at room temperature).

  • Immunohistochemistry (Frozen sections) - Anti-HCN2 antibody [S71] (ab84817)
    Immunohistochemistry (Frozen sections) - Anti-HCN2 antibody [S71] (ab84817)

    Immunohistochemistry (Frozen sections) analysis of mouse brain tissue labelling HCN2 with ab84817 at 1/1000 dilution (1 hour at room temperature). Tissue fixed with 10% Formalin Solution for 12-24 hours at room temperature. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstained with Mayer hematoxylin (purple/blue) nuclear stain at 250-500 µL for 5 minutes at room temperature.

  • Western blot - Anti-HCN2 antibody [S71] (ab84817)
    Western blot - Anti-HCN2 antibody [S71] (ab84817)
    Anti-HCN2 antibody [S71] (ab84817) at 1/1000 dilution + Mouse brain tissue lysate at 15 µg

    Secondary
    Goat anti-mouse IgG, HRP at 1/4000 dilution

    Predicted band size: 95 kDa
    Observed band size: 95 kDa



    Chemiluminescent for HRP (Moss) for 5 min in RT.

  • Western blot - Anti-HCN2 antibody [S71] (ab84817)
    Western blot - Anti-HCN2 antibody [S71] (ab84817)
    Anti-HCN2 antibody [S71] (ab84817) at 1/1000 dilution + Rat brain membrane lysates at 15 µg

    Secondary
    HRP-Sheep Anti-Mouse IgG antibody

    Predicted band size: 95 kDa

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (10)

Publishing research using ab84817? Please let us know so that we can cite the reference in this datasheet.

ab84817 has been referenced in 10 publications.

  • Lei X  et al. Activation of HCN channels caused by elevated cAMP levels in periaqueductal gray promotes bone cancer pain. Neurochem Int 162:105437 (2023). PubMed: 36336089
  • Zhong W & Darmani NA The HCN Channel Blocker ZD7288 Induces Emesis in the Least Shrew (Cryptotis parva). Front Pharmacol 12:647021 (2021). PubMed: 33995059
  • Zhang X  et al. Shank3 contributes to neuropathic pain by facilitating the SNI-dependent increase of HCN2 and the expression of PSD95. Neurosci Res N/A:N/A (2020). PubMed: 32454040
  • Zhao H  et al. Overexpression of the medium-conductance calcium-activated potassium channel (SK4) and the HCN2 channel to generate a biological pacemaker. Mol Med Rep 20:3406-3414 (2019). PubMed: 31432175
  • Mader F  et al. Hyperpolarization-Activated Cyclic Nucleotide-Gated Non-selective (HCN) Ion Channels Regulate Human and Murine Urinary Bladder Contractility. Front Physiol 9:753 (2018). PubMed: 29971015
  • Ding W  et al. Increased HCN Channel Activity in the Gasserian Ganglion Contributes to Trigeminal Neuropathic Pain. J Pain 19:626-634 (2018). PubMed: 29366880
  • Guo T  et al. Association between hyperpolarization-activated channel in interstitial cells of Cajal and gastrointestinal dysmotility induced by malignant ascites. Oncol Lett 13:1601-1608 (2017). IHC-Fr ; Mouse . PubMed: 28454297
  • Pitcairn E  et al. Coordinating heart morphogenesis: A novel role for hyperpolarization-activated cyclic nucleotide-gated (HCN) channels during cardiogenesis inXenopus laevis. Commun Integr Biol 10:e1309488 (2017). PubMed: 28702127
  • Ding W  et al. Inhibition of HCN channel activity in the thalamus attenuates chronic pain in rats. Neurosci Lett 631:97-103 (2016). PubMed: 27542339
  • Feng QF  et al. The effects of acupuncture on bladder interstitial cells of cajal excitability in rats with overactive bladder. Evid Based Complement Alternat Med 2013:261217 (2013). IHC . PubMed: 24194780

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-HCN2 antibody [S71]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (atrium)
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-12%)
Loading amount
50 µg
Specification
atrium
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Christopher Trummer

Verified customer

Submitted Oct 04 2018

Western blot abreview for Anti-HCN2 antibody [S71]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (atrium)
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-12%)
Loading amount
50 µg
Specification
atrium
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Christopher Trummer

Verified customer

Submitted Oct 04 2018

Western blot abreview for Anti-HCN2 antibody [S71]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (atrium)
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-12%)
Loading amount
50 µg
Specification
atrium
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Christopher Trummer

Verified customer

Submitted Oct 04 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-HCN2 antibody [S71-37]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (HL-1 cardiac cell line)
Specification
HL-1 cardiac cell line
Fixative
Methanol
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 04 2010

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