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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-HDAC1 antibody [EPR460(2)] (ab109411)

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Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Immunohistochemistry (Frozen sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Flow Cytometry (Intracellular) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Immunohistochemistry (Frozen sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
  • Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR460(2)] to HDAC1
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, IHC-Fr, ICC/IF, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 APC Carrier Free HRP PE

You may also be interested in

Protein
Product image
Recombinant human HDAC1 protein (ab101661)
Kit
Product image
Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) (ab156064)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-HDAC1 antibody [EPR460(2)]
    See all HDAC1 primary antibodies
  • Description

    Rabbit monoclonal [EPR460(2)] to HDAC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WB, IHC-Fr, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-Fr: Mouse hippocampus tissue. ICC/IF: NIH/3T3, HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR460(2)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • HDACs
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class I
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Isotype/Loading Controls
    • Loading Controls
    • HDAC1

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-HDAC1 antibody [EPR460(2)] (ab192469)
    • Alexa Fluor® 647 Anti-HDAC1 antibody [EPR460(2)] (ab192470)
    • Anti-HDAC1 antibody [EPR460(2)] - BSA and Azide free (ab213701)
  • Assay kits

    • Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) (ab156064)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human HDAC1 protein (ab101661)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/50.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB (4)
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IHC-Fr
1/500.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

ICC/IF (2)
1/50.
IP
1/30.
Notes
Flow Cyt (Intra)
1/50.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IHC-Fr
1/500.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

ICC/IF
1/50.
IP
1/30.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
  • Tissue specificity

    Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications

    Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
    Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q13547 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3065 Human
    • Entrez Gene: 433759 Mouse
    • Entrez Gene: 297893 Rat
    • Omim: 601241 Human
    • SwissProt: Q13547 Human
    • SwissProt: O09106 Mouse
    • SwissProt: Q4QQW4 Rat
    • Unigene: 88556 Human
    • Unigene: 202504 Mouse
    • Unigene: 391033 Mouse
    • Unigene: 1863 Rat
    see all
  • Alternative names

    • DKFZp686H12203 antibody
    • GON 10 antibody
    • HD1 antibody
    • HDAC 1 antibody
    • HDAC1 antibody
    • HDAC1_HUMAN antibody
    • Histone deacetylase 1 antibody
    • Reduced potassium dependency yeast homolog like 1 antibody
    • RPD3 antibody
    • RPD3L1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
    Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling HDAC1 with ab109411 at 1/50 (10.92 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing mainly nuclear staining in HeLa cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
    Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling HDAC1 with ab109411 at 1/50 (10.92 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2ug/ml dilution.

  • Immunohistochemistry (Frozen sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
    Immunohistochemistry (Frozen sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus tissue labeling HDAC1 with ab109411 at 1/500 (5.55 ug/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution (Green). Nuclear staining on mouse hippocampus. is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
    Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
    All lanes : Anti-HDAC1 antibody [EPR460(2)] (ab109411) at 1/1000 dilution (Purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 55 kDa

  • Flow Cytometry (Intracellular) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
    Flow Cytometry (Intracellular) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labelling HDAC1 with Purified ab109411 at 1:20 dilution (5 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
    Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

    Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling HDAC1 with Purified ab109411 at 1:50 dilution (2.4 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue sections labeling HDAC1 with Purified ab109411 at 1:100 dilution (1.2 µg/ml). Heat mediated antigen retrieval was performed using . Tissue was counterstained with Hematoxylin. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue sections labeling HDAC1 with Purified ab109411 at 1:100 dilution (1.2 µg/ml). Heat mediated antigen retrieval was performed using . Tissue was counterstained with Hematoxylin. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling HDAC1 with Purified ab109411 at 1:100 dilution (1.2 µg/ml). Heat mediated antigen retrieval was performed using . Tissue was counterstained with Hematoxylin. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. 

  • Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
    Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Human breast carcinoma lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab109411 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab109411 was shown to recognize HDAC1 when HDAC1 knockout samples were used, along with additional cross-reactive bands. Wild-type and HDAC1 knockout samples were subjected to SDS-PAGE. ab109411 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging

  • Immunohistochemistry (Frozen sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
    Immunohistochemistry (Frozen sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

    Immunohistochemical analysis of 4% PFA fixed, 0.2% Triton X-100 permeabilised Mouse hippocampus staining HDAC1 with ab109411 at 1/500 dilution (5.55 μg/ml). ab150077 AlexaFluor®488 Goat anti-Rabbit was used as a secondary at 1/1000 (2 μg/ml) dilution. Nuclear counterstain: DAPI. 

    Nuclear staining on mouse hippocampus.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

  • Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)
    Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Human breast carcinoma lysate (20 µg) or K562 lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab109411 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.

    This western blot image is a comparison between ab109411 and a competitor's top cited rabbit polyclonal antibody.

  • Anti-HDAC1 antibody [EPR460(2)] (ab109411)
    Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (21)

Publishing research using ab109411? Please let us know so that we can cite the reference in this datasheet.

ab109411 has been referenced in 21 publications.

  • Sedaka R  et al. High salt intake induces collecting duct HDAC1-dependent NO signaling. Am J Physiol Renal Physiol 320:F297-F307 (2021). PubMed: 33356953
  • Lin S  et al. Differential MUC22 expression by epigenetic alterations in human lung squamous cell carcinoma and adenocarcinoma. Oncol Rep 45:N/A (2021). PubMed: 33786615
  • Maresca A  et al. DNMT1 mutations leading to neurodegeneration paradoxically reflect on mitochondrial metabolism. Hum Mol Genet 29:1864-1881 (2020). PubMed: 31984424
  • Turnbull RE  et al. The MiDAC histone deacetylase complex is essential for embryonic development and has a unique multivalent structure. Nat Commun 11:3252 (2020). PubMed: 32591534
  • Horikawa N  et al. Anti-VEGF therapy resistance in ovarian cancer is caused by GM-CSF-induced myeloid-derived suppressor cell recruitment. Br J Cancer 122:778-788 (2020). PubMed: 31932754
View all Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Brain)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
12 µg
Treatment
Aliquoted and heated from 34C to 69C to determine melting temperature of HDAC1. Lysate centrifuged at 18,000 x g to remove aggregates after melting. Supernatent collected, 3X SDS PAGE loading dye added, and heated sampled were heated to 95C for 10 min. Note, input sample was not heated before centrifugation, but was heated to 95C after loading dye added.
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 17 2015

Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-12% Tris-bis glycine gel)
Sample
Mouse Tissue lysate - other (mouse brain tissue)
Specification
mouse brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Arjang Salehi

Verified customer

Submitted Mar 26 2015

Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - nuclear (B cells)
Specification
B cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 16 2015

Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

Average
Abreviews
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (heart)
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 13 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-HDAC1 antibody [EPR460(2)]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 28°C
Sample
Mouse Cell (Neural cell)
Specification
Neural cell
Permeabilization
Yes - 0.3% TritonX-100 in PBS for 30 minutes
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 13 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-HDAC1 antibody [EPR460(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

Submitted May 31 2012

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