Recombinant Anti-HDAC6 antibody [EPR22951-29] - BSA and Azide free (ab264565)
![Immunoprecipitation - Anti-HDAC6 antibody [EPR22951-29] - BSA and Azide free (ab264565)](https://www.abcam.com/ps/products/264/ab264565/Images/ab264565-3-anti-hdac6-antibody-epr22951-29-immunoprecipitation-brain-mouse.jpg "Immunoprecipitation - Anti-HDAC6 antibody [EPR22951-29] - BSA and Azide free (ab264565)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22951-29] to HDAC6 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
-
Product name
Anti-HDAC6 antibody [EPR22951-29] - BSA and Azide free
See all HDAC6 primary antibodies -
Description
Rabbit monoclonal [EPR22951-29] to HDAC6 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Mouse and rat brain lysate. NIH/3T3, Neuro-2a and PC-12 lysates. Flow Cyt (intra): Neuro-2a and NIH/3T3 cells. IP: Mouse brain lysate and NIH/3T3 lysate.
-
General notes
ab264565 is the carrier-free version of ab239362.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22951-29 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 131 kDa.
|
|
| IP |
1/30.
|
| Notes |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 131 kDa. |
|
IP
1/30. |
Target
-
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger. -
Post-translational
modificationsUbiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro. -
Cellular localization
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules. - Information by UniProt
-
Database links
- Entrez Gene: 15185 Mouse
- Entrez Gene: 84581 Rat
- SwissProt: Q9Z2V5 Mouse
- Unigene: 29854 Mouse
-
Alternative names
- CPBHM antibody
- FLJ16239 antibody
- HD 6 antibody
see all
Images
-
Immunoprecipitation - Anti-HDAC6 antibody [EPR22951-29] - BSA and Azide free (ab264565)
HDAC6 was immunoprecipitated from 0.35 mg mouse brain lysate 10µg with ab239362 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239362 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain lysate 10µg
Lane 2: ab239362 IP in mouse brain lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab239362 in mouse brain lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239362).
-
![Flow Cytometry (Intracellular) - Anti-HDAC6 antibody [EPR22951-29] - BSA and Azide free (ab264565)](https://www.abcam.com/ps/products/264/ab264565/Images/ab264565-2-anti-hdac6-antibody-epr22951-29-flowcytometry-nih-3t3-mouse.jpg)
Flow Cytometry (Intracellular) - Anti-HDAC6 antibody [EPR22951-29] - BSA and Azide free (ab264565)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labeling HDAC6 with ab239362 at 1\600 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239362).
-
![Flow Cytometry (Intracellular) - Anti-HDAC6 antibody [EPR22951-29] - BSA and Azide free (ab264565)](https://www.abcam.com/ps/products/264/ab264565/Images/ab264565-1-anti-hdac6-antibody-epr22951-29-flowcytometry-neuro2a-mouse.jpg)
Flow Cytometry (Intracellular) - Anti-HDAC6 antibody [EPR22951-29] - BSA and Azide free (ab264565)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling HDAC6 with ab239362 at 1\600 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239362).
-
![Anti-HDAC6 antibody [EPR22951-29] - BSA and Azide free (ab264565)](https://www.abcam.com/ps/products/264/ab264565/Images/ab264565-4-benefits-of-recombinant-antibodies.png)
Anti-HDAC6 antibody [EPR22951-29] - BSA and Azide free (ab264565)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab264565 has not yet been referenced specifically in any publications.
