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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)

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Western blot - Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)
  • Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)
  • Flow Cytometry (Intracellular) - Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)
  • Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10338(2)] to HDAC8 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free
    See all HDAC8 primary antibodies

  • Description

    Rabbit monoclonal [EPR10338(2)] to HDAC8 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, K562 and HeLa whole cell lysates.

  • General notes

    ab232643 is the carrier-free version of ab187139.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab232643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
IP
Use at an assay dependent concentration.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
IP
Use at an assay dependent concentration.

Target

  • Function

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.

  • Tissue specificity

    Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.

  • Sequence similarities

    Belongs to the histone deacetylase family. HD type 1 subfamily.

  • Post-translational
    modifications

    Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.

  • Cellular localization

    Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CDA07 antibody
    • CDLS5 antibody
    • HD 8 antibody
    • HD8 antibody
    • HDAC 8 antibody
    • Hdac8 antibody
    • HDAC8_HUMAN antibody
    • HDACL 1 antibody
    • HDACL1 antibody
    • Histone deacetylase 8 antibody
    • Histone deacetylase like 1 antibody
    • MRXS6 antibody
    • RPD 3 antibody
    • RPD3 antibody
    • WTS antibody
    see all

Images

  • Western blot - Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)
    Western blot - Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: HDAC8 knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate (20 µg)
    Lane 4: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab187139. Red - loading control, ab7291, observed at 52 kDa.
    ab187139 was shown to specifically react with HDAC8 when HDAC8 knockout samples were used. Wild-type and HDAC8 knockout samples were subjected to SDS-PAGE. ab187139 and ab7291 (loading control to alpha tubulin) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187139).

  • Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)
    Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)

    Immunoprecipitation analysis of human fetal kidney tissue lysate labeling HDAC8 using ab187139 at 1/50 dilution (Lane 1). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of human fetal kidney tissue lysate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187139).

  • Flow Cytometry (Intracellular) - Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)
    Flow Cytometry (Intracellular) - Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)

    Intracellular Flow Cytometry analysis of K562 cells labeling HDAC8 using ab187139 at 1/150 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187139). 

     

     

  • Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)
    Anti-HDAC8 antibody [EPR10338(2)] - BSA and Azide free (ab232643)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab232643? Please let us know so that we can cite the reference in this datasheet.

ab232643 has not yet been referenced specifically in any publications.

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