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    products/primary-antibodies/heavy-chain-cardiac-myosin-antibody-ba-g5-bsa-and-azide-free-ab174640.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Myosin
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Anti-heavy chain cardiac Myosin antibody [BA-G5] - BSA and Azide free (ab174640)

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Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] - BSA and Azide free (ab174640)

    Key features and details

    • Mouse monoclonal [BA-G5] to heavy chain cardiac Myosin - BSA and Azide free
    • Suitable for: WB, IHC-P, IHC-FoFr
    • Reacts with: Mouse
    • Isotype: IgG2b

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    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Primary
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    Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)

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    Overview

    • Product name

      Anti-heavy chain cardiac Myosin antibody [BA-G5] - BSA and Azide free
      See all heavy chain cardiac Myosin primary antibodies
    • Description

      Mouse monoclonal [BA-G5] to heavy chain cardiac Myosin - BSA and Azide free
    • Host species

      Mouse
    • Tested applications

      Suitable for: WB, IHC-P, IHC-FoFrmore details
    • Species reactivity

      Reacts with: Mouse
      Predicted to work with: Rat, Rabbit, Cow, Human
    • Immunogen

      Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

    • Positive control

      • This antibody gave a positive signal in the following Tissue Lysates: Mouse Skeletal Muscle, Rat Heart, Rat Skeletal Muscle
    • General notes

      ab174640 is the carrier-free version of ab50967.

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Constituent: PBS
    • Carrier free

      Yes
    • Concentration information loading...
    • Purity

      IgG fraction
    • Clonality

      Monoclonal
    • Clone number

      BA-G5
    • Isotype

      IgG2b
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Motor Proteins
      • Myosin
      • Immunology
      • Immune System Diseases
      • Autoimmune
      • Cardiovascular
      • Heart
      • Contractility
      • Contractile Proteins
      • Myosins

    Associated products

    • Alternative Versions

      • Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
    • Isotype control

      • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab174640 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 5 µg/ml. Predicted molecular weight: 223 kDa.
    IHC-P
    1/100.
    IHC-FoFr
    Use at an assay dependent concentration.
    Notes
    WB
    Use a concentration of 5 µg/ml. Predicted molecular weight: 223 kDa.
    IHC-P
    1/100.
    IHC-FoFr
    Use at an assay dependent concentration.

    Images

    • Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] - BSA and Azide free (ab174640)
      Western blot - Anti-heavy chain cardiac Myosin antibody [BA-G5] - BSA and Azide free (ab174640)
      Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 5 µg/ml + Heart (Mouse) Tissue Lysate at 10 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Mouse IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 223 kDa
      Observed band size: 223 kDa



      This WB data was generated using the same antibody clone in a different buffer formulation (ab50967). 

    Protocols

    • Mouse on Mouse staining protocol

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab174640? Please let us know so that we can cite the reference in this datasheet.

    ab174640 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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