Anti-heavy chain cardiac Myosin antibody [BA-G5] - BSA and Azide free (ab174640)
Key features and details
- Mouse monoclonal [BA-G5] to heavy chain cardiac Myosin - BSA and Azide free
- Suitable for: WB, IHC-P, IHC-FoFr
- Reacts with: Mouse
- Isotype: IgG2b
Overview
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Product name
Anti-heavy chain cardiac Myosin antibody [BA-G5] - BSA and Azide free
See all heavy chain cardiac Myosin primary antibodies -
Description
Mouse monoclonal [BA-G5] to heavy chain cardiac Myosin - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Rabbit, Cow, Human -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following Tissue Lysates: Mouse Skeletal Muscle, Rat Heart, Rat Skeletal Muscle
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General notes
ab174640 is the carrier-free version of ab50967.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
BA-G5 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 5 µg/ml. Predicted molecular weight: 223 kDa.
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IHC-P |
1/100.
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IHC-FoFr |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 5 µg/ml. Predicted molecular weight: 223 kDa. |
IHC-P
1/100. |
IHC-FoFr
Use at an assay dependent concentration. |
Images
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Anti-heavy chain cardiac Myosin antibody [BA-G5] (ab50967) at 5 µg/ml + Heart (Mouse) Tissue Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Mouse IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 223 kDa
Observed band size: 223 kDaThis WB data was generated using the same antibody clone in a different buffer formulation (ab50967).
Datasheets and documents
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Datasheet download
References (0)
ab174640 has not yet been referenced specifically in any publications.