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  1. Link

    products/primary-antibodies/heavy-chain-myosinmyh3-antibody-f1652-ab264038.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Myosin
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Recombinant

Recombinant Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
  • Western blot - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
  • Western blot - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
  • Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [F1.652] to heavy chain Myosin/MYH3
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

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Peptide
Human Fast Myosin Skeletal Heavy chain peptide (ab102066)
Secondary
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
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Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

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Overview

  • Product name

    Anti-heavy chain Myosin/MYH3 antibody [F1.652]
    See all heavy chain Myosin/MYH3 primary antibodies
  • Description

    Mouse monoclonal [F1.652] to heavy chain Myosin/MYH3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IHC-Fr
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse embryo tissue lysate; L6 whole cell lysate; Mouse 14.5 limb tissue lysate. IHC-P: Mouse 14.5 tissue; Rat 14.5 tissue.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    F1.652
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Myosin

Associated products

  • Alternative Versions

    • Anti-heavy chain Myosin/MYH3 antibody [F1.652] - BSA and Azide free (ab264039)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab264038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.222 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 224 kDa).
IHC-P
Use a concentration of 2.22 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 0.222 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 224 kDa).
IHC-P
Use a concentration of 2.22 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for ICC/IF or IHC-Fr.

Target

  • Function

    Muscle contraction.
  • Involvement in disease

    Defects in MYH3 are the cause of distal arthrogryposis type 2A (DA2A) [MIM:193700]; also known as Freeman-Sheldon syndrome (FSS). Distal arthrogryposis is a clinically and genetically heterogeneous group of disorders characterized by bone anomalies and joint contractures of the hands and feet, causing medially overlapping fingers, clenched fists, ulnar deviation of fingers, camptodactyly and positional foot deformities. It is a disorder of primary limb malformation without primary neurologic or muscle disease. DA2A is the most severe form of distal arthrogryposis. Affected individuals have contractures of the orofacial muscles, characterized by microstomia with pouting lips, H-shaped dimpling of the chin, deep nasolabial folds, and blepharophimosis. Dysphagia, failure to thrive, growth deficit, and life-threatening respiratory complications (caused by structural anomalies of the oropharynx and upper airways) are frequent. Inheritance is autosomal dominant.
    Defects in MYH3 are the cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as Sheldon-Hall syndrome (SHS) or arthrogryposis multiplex congenita distal type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. DA2B is the most common of the distal arthrogryposis syndromes. It is similar to DA2A but the facial contractures are less dramatic.
  • Sequence similarities

    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Developmental stage

    Abundantly present in fetal skeletal muscle and not present or barely detectable in heart and adult skeletal muscle.
  • Domain

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localization

    Cytoplasm > myofibril. Thick filaments of the myofibrils.
  • Target information above from: UniProt accession P11055 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 17883 Mouse
    • Entrez Gene: 24583 Rat
    • SwissProt: P13541 Mouse
    • SwissProt: P12847 Rat
    • Unigene: 340090 Mouse
    • Unigene: 98847 Rat
    • Alternative names

      • embryonic antibody
      • fast skeletal muscle antibody
      • HEMHC antibody
      • Muscle embryonic myosin heavy chain 3 antibody
      • Muscle embryonic myosin heavy chain antibody
      • MYH 3 antibody
      • Myh3 antibody
      • MYH3_HUMAN antibody
      • MYHC EMB antibody
      • MYHSE 1 antibody
      • MYHSE1 antibody
      • Myosin heavy chain 3 antibody
      • Myosin heavy chain 3 skeletal muscle embryonic antibody
      • Myosin heavy chain antibody
      • Myosin heavy chain fast skeletal muscle embryonic antibody
      • Myosin Heavy Polypeptide 3 antibody
      • Myosin heavy polypeptide 3 skeletal muscle embryonic antibody
      • Myosin skeletal heavy chain embryonic 1 antibody
      • Myosin-3 antibody
      • SMHCE antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)

      Immunohistochemical analysis of paraffin-embedded mouse E14.5 tissue labeling Myosin (MYH3) with ab264038 at 1/500 dilution (2.22 µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on the skeletal muscle of mouse E14.5. The section was incubated with ab264038 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Western blot - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
      Western blot - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
      All lanes : Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038) at 0.222 µg/ml

      Lane 1 : Mouse embryo tissue lysate
      Lane 2 : Mouse adult skeletal muscle tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

      Predicted band size: 224 kDa
      Observed band size: 250 kDa why is the actual band size different from the predicted?


      Exposure time: 26 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      Negative control: Mouse adult skelatal muscle (PMID:3530499).


      The expression profile observed is consistent with what has been described in the literature (PMID: 3530499, 12899730).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)

      Immunohistochemical analysis of paraffin-embedded rat E14.5 tissue labeling Myosin (MYH3) with ab264038 at 1/500 dilution (2.22 µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on the skeletal muscle of rat E14.5. The section was incubated with ab264038 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Western blot - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
      Western blot - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
      All lanes : Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038) at 0.222 µg/ml

      Lane 1 : L6 (rat skeletal muscle myoblast), whole cell lysate
      Lane 2 : Mouse E14.5 limb tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

      Predicted band size: 224 kDa
      Observed band size: 250 kDa why is the actual band size different from the predicted?


      Exposure time: 26 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The expression profile observed is consistent with what has been described in the literature (PMID: 3530499, 12899730).

    • Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
      Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab264038? Please let us know so that we can cite the reference in this datasheet.

    ab264038 has been referenced in 1 publication.

    • Silberstein L  et al. Developmental progression of myosin gene expression in cultured muscle cells. Cell 46:1075-81 (1986). PubMed: 3530499

    Customer reviews and Q&As

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