Recombinant Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)](https://www.abcam.com/ps/products/264/ab264038/Images/ab264038-1-anti-myosin-myh3-antibody-f1652-immunohistochemistry-mouse-e145.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [F1.652] to heavy chain Myosin/MYH3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-heavy chain Myosin/MYH3 antibody [F1.652]
See all heavy chain Myosin/MYH3 primary antibodies -
Description
Mouse monoclonal [F1.652] to heavy chain Myosin/MYH3 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IHC-Fr -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse embryo tissue lysate; L6 whole cell lysate; Mouse 14.5 limb tissue lysate. IHC-P: Mouse 14.5 tissue; Rat 14.5 tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
F1.652 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.222 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 224 kDa).
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| IHC-P |
Use a concentration of 2.22 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
Use a concentration of 0.222 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 224 kDa). |
|
IHC-P
Use a concentration of 2.22 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Muscle contraction. -
Involvement in disease
Defects in MYH3 are the cause of distal arthrogryposis type 2A (DA2A) [MIM:193700]; also known as Freeman-Sheldon syndrome (FSS). Distal arthrogryposis is a clinically and genetically heterogeneous group of disorders characterized by bone anomalies and joint contractures of the hands and feet, causing medially overlapping fingers, clenched fists, ulnar deviation of fingers, camptodactyly and positional foot deformities. It is a disorder of primary limb malformation without primary neurologic or muscle disease. DA2A is the most severe form of distal arthrogryposis. Affected individuals have contractures of the orofacial muscles, characterized by microstomia with pouting lips, H-shaped dimpling of the chin, deep nasolabial folds, and blepharophimosis. Dysphagia, failure to thrive, growth deficit, and life-threatening respiratory complications (caused by structural anomalies of the oropharynx and upper airways) are frequent. Inheritance is autosomal dominant.
Defects in MYH3 are the cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as Sheldon-Hall syndrome (SHS) or arthrogryposis multiplex congenita distal type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. DA2B is the most common of the distal arthrogryposis syndromes. It is similar to DA2A but the facial contractures are less dramatic. -
Sequence similarities
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
Developmental stage
Abundantly present in fetal skeletal muscle and not present or barely detectable in heart and adult skeletal muscle. -
Domain
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
Cellular localization
Cytoplasm > myofibril. Thick filaments of the myofibrils. - Information by UniProt
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Database links
- Entrez Gene: 17883 Mouse
- Entrez Gene: 24583 Rat
- SwissProt: P13541 Mouse
- SwissProt: P12847 Rat
- Unigene: 340090 Mouse
- Unigene: 98847 Rat
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Alternative names
- embryonic antibody
- fast skeletal muscle antibody
- HEMHC antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
Immunohistochemical analysis of paraffin-embedded mouse E14.5 tissue labeling Myosin (MYH3) with ab264038 at 1/500 dilution (2.22 µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on the skeletal muscle of mouse E14.5. The section was incubated with ab264038 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Western blot - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)](https://www.abcam.com/ps/products/264/ab264038/Images/ab264038-395318-anti-myosin-myh3-antibody-f1652-western-blot-mouse-embryo-tissue-lysate-20ug.jpg)
Western blot - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)All lanes : Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038) at 0.222 µg/ml
Lane 1 : Mouse embryo tissue lysate
Lane 2 : Mouse adult skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 224 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Negative control: Mouse adult skelatal muscle (PMID:3530499).
The expression profile observed is consistent with what has been described in the literature (PMID: 3530499, 12899730). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)](https://www.abcam.com/ps/products/264/ab264038/Images/ab264038-2-anti-myosin-myh3-antibody-f1652-immunohistochemistry-rat-e145.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)Immunohistochemical analysis of paraffin-embedded rat E14.5 tissue labeling Myosin (MYH3) with ab264038 at 1/500 dilution (2.22 µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on the skeletal muscle of rat E14.5. The section was incubated with ab264038 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Western blot - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)](https://www.abcam.com/ps/products/264/ab264038/Images/ab264038-395317-anti-myosin-myh3-antibody-f1652-western-blot-l6.jpg)
Western blot - Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)All lanes : Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038) at 0.222 µg/ml
Lane 1 : L6 (rat skeletal muscle myoblast), whole cell lysate
Lane 2 : Mouse E14.5 limb tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 224 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 3530499, 12899730).
-
![Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)](https://www.abcam.com/ps/products/264/ab264038/Images/ab264038-5-benefits-of-recombinant-antibodies.png)
Anti-heavy chain Myosin/MYH3 antibody [F1.652] (ab264038)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab264038 has been referenced in 1 publication.
- Silberstein L et al. Developmental progression of myosin gene expression in cultured muscle cells. Cell 46:1075-81 (1986). PubMed: 3530499