Anti-HEC1/HEC antibody [9G3] (ab3613)

In general, issues like this arise from the secondary antibody. Often the antibody is too concentrated or is reacting with something in you buffers (usually milk or BSA) Have you had the opportunity to reduce the concentration of the secondary antibody when using this product or have you tried changing your blocking/incubation buffer? You may sometimes see these types of problem before film exposure by looking at the membrane in a darkroom after ECL. If the membrane is glowing then you should allow it to 'cool down' for up to 1 hour before exposure to film. I was also wondering if you could let me know what type of ECL reagent you are using? A high intensity ECL reagent like the Pierce Pico or Dura, GE Prime or Abcam Optiblot Ultradetect may be too sensitive for use with this product. If using a high sensitivity product we suggest switching to a lower sensitivity ECL reagent.

Thank you for enquiry and your interest.

I can confirm that this antibody (ab3613) recognizes Mouse, Human, Pig, African Green Monkey - it has not been tested in yeast and currently we do not have antibodies which detect HEC1 in Saccharomyces.

If you need any further assistance in the future, please do not hesitate to contact me.

I appreciate your cooperation and understand your concerns. It is regrettable that this product has not matched the standards of those previously.

Under our Abpromise, we guarantee that our product will work in a the manner stated on the datasheet, e.g. Western Blot on porcine tissue. If the product has failed to do so, we offer scientific support, replace or refund of the product. Our Abpromise covers the product for 6 months.

The details requested are nessesary in order for us to determine whether this product is covered by our Abpromise. If you could provide some information, such as species type or protocol conditions this would be very helpful. We appreciate your time taken to supply us this information. This information will also be helpful to our quality monitoring.

I will be pleased to provide a free of charge replacmenet or credit note if the antibody is not working as it should be and has been purchased within the last 6 months. In order to process this I will need the Abcam order confirmation number or the PO number used to purchase the product.

Thank you for your time. I look forward to hearing from you with the requested information and hope we can resolve this case as soon as possible.

Thank you for contacting Abcam.

I have called and left a message with you in regards to this issue. Feel free to disregard that call should this be a better forum for communicating.

I am sorry that the latest lot of this product is not performing well for you. Before I may issue a replacement however I was hoping that you could please send me the protocol that you have used, any troubleshooting steps that you might have performed and any images should you have them. Please be as specific as possible as this data will help us pinpoint issues that the antibody might have and test them as necessary.

Please let me know if you have any questions.

Thank you very much for your phone call. The dilution given on the datasheet is for guideline purpose only. The dilutions have to be optimized by the end user based on cells or tissue sections they use. We recommend using a range e.g. 1/500, 1/1000 and 1/2000 dilution; this range will helps to get best optimized results at particular dilutions. 1/1000 dilution =1 ul of antibody diluted in 999 ul of buffer (1-5%BSA + PBS). or 10 ul of antibody solution in 9990 ul of buffer will give the same 1/1000 dilution with total volume of 10ml. We recommend aliquoting 10 ul per vial and storing the antibody neat at -20C or -80C (as recommended on datasheet). Antibodies can also be diluted in PBS with 1-3% BSAin casethere is need for diluted antibody to be stored. I hope this info will be helpful. SHould you have any other inquiry please do not hesitate to contact us.