Recombinant Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3608] to Hemoglobin subunit alpha
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Hemoglobin subunit alpha antibody [EPR3608]
See all Hemoglobin subunit alpha primary antibodies -
Description
Rabbit monoclonal [EPR3608] to Hemoglobin subunit alpha -
Host species
Rabbit -
Specificity
This antibody gave a positive signal in Western blotting of rat brain, heart, kidney and spleen tissue. This antibody works in human, mouse and rat in IHC. -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Hemoglobin subunit alpha aa 50-150.
(Peptide available asab206105) -
Positive control
- K562 cell lysate, human heart lysate, human spleen tissue, human stomach cancer tissue, human lung tissue, human placenta, rat heart lysate, mouse spleen lysate and mouse cerebral cortex.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3608 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Hemoglobin subunit alpha antibody [EPR3608] - BSA and Azide free (ab174536)
- Alexa Fluor® 647 Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab215919)
- Biotin Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab217074)
- Alexa Fluor® 568 Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab313122)
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).Can be blocked with Hemoglobin subunit alpha peptide (ab206105).
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).Can be blocked with Hemoglobin subunit alpha peptide (ab206105). |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Target
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Function
Involved in oxygen transport from the lung to the various peripheral tissues. -
Tissue specificity
Red blood cells. -
Involvement in disease
Heinz body anemias
Alpha-thalassemia
Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
Hemoglobin H disease -
Sequence similarities
Belongs to the globin family. -
Post-translational
modificationsThe initiator Met is not cleaved in variant Thionville and is acetylated. - Information by UniProt
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Database links
- Entrez Gene: 3039 Human
- Entrez Gene: 3040 Human
- Omim: 141800 Human
- Omim: 141850 Human
- SwissProt: P69905 Human
- SwissProt: P01942 Mouse
- SwissProt: P01946 Rat
- Unigene: 449630 Human
see all -
Alternative names
- Alpha 1 globin antibody
- Alpha globin antibody
- Alpha one globin antibody
see all
Images
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All lanes : Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492) at 1/2000 dilution (purified)
Lane 1 : Human heart lysate
Lane 2 : Rat heart lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labeling Hemoglobin subunit alpha antibody with purified ab92492 at 1/500 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody at 1/500 dilution. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labeling Hemoglobin subunit alpha antibody with purified ab92492 at 1/500 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody at 1/500 dilution. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492) at 1/10000 dilution (purified) + Mouse spleen lysate at 15 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM /TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebral cortex tissue labeling Hemoglobin subunit alpha antibody with purified ab92492 at 1/500 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody at 1/500 dilution. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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All lanes : Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492) at 1/1000 dilution (unpurified)
Lane 1 : K562 cell lysate
Lane 2 : Human heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin embedded human placenta tissue labeling hemoglobin subunit alpha with unpurified ab92492 at 0.051 µg/ml (1:40000 dilution).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded human stomach cancer tissue labeling hemoglobin subunit alpha with unpurified ab92492 at 0.051 µg/ml (1:40000 dilution).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded human placenta tissue labeling hemoglobin subunit alpha with unpurified ab92492 at 0.051 µg/ml (1:40000 dilution).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab92492 has been referenced in 23 publications.
- Baine RE et al. Noxious Stimulation Induces Acute Hemorrhage and Impairs Long-Term Recovery after Spinal Cord Injury (SCI) in Female Rats: Evidence Estrous Cycle May Have a Modulatory Effect. Neurotrauma Rep 3:70-86 (2022). PubMed: 35112109
- Nahaboo W et al. Keratin filaments mediate the expansion of extra-embryonic membranes in the post-gastrulation mouse embryo. EMBO J 41:e108747 (2022). PubMed: 35266581
- Hauke M et al. Active RhoA Exerts an Inhibitory Effect on the Homeostasis and Angiogenic Capacity of Human Endothelial Cells. J Am Heart Assoc 11:e025119 (2022). PubMed: 35699166
- Rahrmann EP et al. The NALCN channel regulates metastasis and nonmalignant cell dissemination. Nat Genet 54:1827-1838 (2022). PubMed: 36175792
- Tang TT et al. Kim-1 Targeted Extracellular Vesicles: A New Therapeutic Platform for RNAi to Treat AKI. J Am Soc Nephrol N/A:N/A (2021). PubMed: 34127536