Recombinant Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
![Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)](https://www.abcam.com/ps/products/131/ab131225/Images/ab131225-469365-anti-hemoglobin-subunit-beta--delta-wb-recombinat-protein.JPG "Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8322(B)] to Hemoglobin subunit delta + Hemoglobin subunit beta
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] -
Description
Rabbit monoclonal [EPR8322(B)] to Hemoglobin subunit delta + Hemoglobin subunit beta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Hemoglobin subunit delta aa 1-100. The exact sequence is proprietary.
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Positive control
- Human fetal heart, fetal liver, and red blood cell (RBC) lysates; Human endometrium and spleen tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8322(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/200 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/200 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Images
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Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)All lanes : Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225) at 1/200 dilution
Lane 1 :Recombinant Human Hemoglobin subunit alpha protein (denatured) (ab131697)
Lane 2 : His-tagged human Hemoglobin subunit beta/ba1 (aa 3-147) recombinant protein
Lane 3 : His-tagged human Hemoglobin subunit delta (aa 3-147) recombinant protein
Lane 4 : His-tagged full length human Hemoglobin subunit delta recombinant protein
Lane 5 : His-tagged human Hemoglobin subunit epsilon (aa 3-147) recombinant protein
Lane 6 : His-tagged human Hemoglobin subunit gamma-2 (aa 2-147) recombinant protein
Lane 7 : His-tagged human Hemoglobin subunit gamma-1 (aa 2-147) recombinant protein
Lane 8 :Recombinant Human Hemoglobin subunit zeta protein (ab95347)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 16 kDaBlocking/Dilutiing buffer and concentration: 5% NFDM/TBST
Exposure time: 100 seconds
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![Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)](https://www.abcam.com/ps/products/131/ab131225/Images/ab131225-145535-anti-hemoglobin-subunit-delta-antibody-epr8322b-western-blot.jpg)
Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)All lanes : Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : Human red blood cell (RBC) lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)](https://www.abcam.com/ps/products/131/ab131225/Images/ab131225-145534-anti-hemoglobin-subunit-delta-antibody-epr8322b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)Immunohistochemical analysis of paraffin embedded Human endometrium tissue labelling Hemoglobin subunit delta with ab131225 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)](https://www.abcam.com/ps/products/131/ab131225/Images/ab131225-145533-anti-hemoglobin-subunit-delta-antibody-epr8322b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling Hemoglobin subunit delta with ab131225 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)](https://www.abcam.com/ps/products/131/ab131225/Images/ab131225-4-benefits-of-recombinant-antibodies.png)
Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab131225 has been referenced in 1 publication.
- Zhang X et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873