RecombinantRabMAb

Recombinant Anti-Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)

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Western blot - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
  • Western blot - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
  • Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8322(B)] to Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Carrier Free

Overview

  • Product name

    Anti-Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta antibody [EPR8322(B)]

  • Description

    Rabbit monoclonal [EPR8322(B)] to Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Hemoglobin subunit delta aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Human fetal heart, fetal liver, and red blood cell (RBC) lysates; Human endometrium and spleen tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/200 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/200 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for IP.

Target

Images

  • Western blot - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
    Western blot - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
    All lanes : Anti-Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225) at 1/200 dilution

    Lane 1 : Recombinant Human Hemoglobin subunit alpha protein (denatured) (ab131697)
    Lane 2 : His-tagged human Hemoglobin subunit beta/ba1 (aa 3-147) recombinant protein
    Lane 3 : His-tagged human Hemoglobin subunit delta (aa 3-147) recombinant protein
    Lane 4 : His-tagged full length human Hemoglobin subunit delta recombinant protein
    Lane 5 : His-tagged human Hemoglobin subunit epsilon (aa 3-147) recombinant protein
    Lane 6 : His-tagged human Hemoglobin subunit gamma-2 (aa 2-147) recombinant protein
    Lane 7 : His-tagged human Hemoglobin subunit gamma-1 (aa 2-147) recombinant protein
    Lane 8 : Recombinant Human Hemoglobin subunit zeta protein (ab95347)

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 16 kDa



    Blocking/Dilutiing buffer and concentration: 5% NFDM/TBST

    Exposure time: 100 seconds

     

  • Western blot - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
    Western blot - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
    All lanes : Anti-Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225) at 1/1000 dilution

    Lane 1 : Human fetal heart tissue lysate
    Lane 2 : Human fetal liver tissue lysate
    Lane 3 : Human red blood cell (RBC) lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 16 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)

    Immunohistochemical analysis of paraffin embedded Human endometrium tissue labelling Hemoglobin subunit delta with ab131225 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)

    Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling Hemoglobin subunit delta with ab131225 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
    Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)

References (1)

Publishing research using ab131225? Please let us know so that we can cite the reference in this datasheet.

ab131225 has been referenced in 1 publication.

  • Zhang X  et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873

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