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    products/primary-antibodies/hemoglobin-subunit-betaba1--hemoglobin-subunit-delta-antibody-epr8322b-ab131225.pdf

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RecombinantRabMAb

Recombinant Anti-Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)

  • Datasheet
  • SDS
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Western blot - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
  • Western blot - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
  • Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8322(B)] to Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta antibody [EPR8322(B)]
  • Description

    Rabbit monoclonal [EPR8322(B)] to Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Hemoglobin subunit delta aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Human fetal heart, fetal liver, and red blood cell (RBC) lysates; Human endometrium and spleen tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8322(B)
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Other
    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Other
    • Cardiovascular
    • Blood
    • Erythrocytes

Associated products

  • Alternative Versions

    • Anti-Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta antibody [EPR8322(B)] - BSA and Azide fr (ab248384)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/200 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/200 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for IP.

Target

  • Cellular localization

    Hemoglobin subunit beta/ba1: Haemoglobin complex, red blood cells.
  • Database links

    • Entrez Gene: 3043 Human
    • Entrez Gene: 3045 Human
    • Omim: 141900 Human
    • Omim: 142000 Human
    • SwissProt: P02042 Human
    • SwissProt: P68871 Human
    • Unigene: 699280 Human

    Images

    • Western blot - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
      Western blot - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
      All lanes : Anti-Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225) at 1/200 dilution

      Lane 1 : Recombinant Human Hemoglobin subunit alpha protein (denatured) (ab131697)
      Lane 2 : His-tagged human Hemoglobin subunit beta/ba1 (aa 3-147) recombinant protein
      Lane 3 : His-tagged human Hemoglobin subunit delta (aa 3-147) recombinant protein
      Lane 4 : His-tagged full length human Hemoglobin subunit delta recombinant protein
      Lane 5 : His-tagged human Hemoglobin subunit epsilon (aa 3-147) recombinant protein
      Lane 6 : His-tagged human Hemoglobin subunit gamma-2 (aa 2-147) recombinant protein
      Lane 7 : His-tagged human Hemoglobin subunit gamma-1 (aa 2-147) recombinant protein
      Lane 8 : Recombinant Human Hemoglobin subunit zeta protein (ab95347)

      Lysates/proteins at 0.1 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 16 kDa



      Blocking/Dilutiing buffer and concentration: 5% NFDM/TBST

      Exposure time: 100 seconds

       

    • Western blot - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
      Western blot - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
      All lanes : Anti-Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225) at 1/1000 dilution

      Lane 1 : Human fetal heart tissue lysate
      Lane 2 : Human fetal liver tissue lysate
      Lane 3 : Human red blood cell (RBC) lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 16 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)

      Immunohistochemical analysis of paraffin embedded Human endometrium tissue labelling Hemoglobin subunit delta with ab131225 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)

      Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling Hemoglobin subunit delta with ab131225 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
      Anti-Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab131225? Please let us know so that we can cite the reference in this datasheet.

    ab131225 has been referenced in 1 publication.

    • Zhang X  et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab131225.
    Please use the links above to contact us or submit feedback about this product.

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