Recombinant Anti-Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta antibody [EPR8322(B)] - BSA and Azide fr (ab248384)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8322(B)] to Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta antibody [EPR8322(B)] - BSA and Azide fr -
Description
Rabbit monoclonal [EPR8322(B)] to Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248384 is the carrier-free version of ab131225.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8322(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Cellular localization
Hemoglobin subunit beta/ba1: Haemoglobin complex, red blood cells. -
Database links
- Entrez Gene: 3043 Human
- Entrez Gene: 3045 Human
- Omim: 141900 Human
- Omim: 142000 Human
- SwissProt: P02042 Human
- SwissProt: P68871 Human
- Unigene: 699280 Human
Images
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All lanes : Anti-Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225) at 1/200 dilution
Lane 1 :Recombinant Human Hemoglobin subunit alpha protein (denatured) (ab131697)
Lane 2 : His-tagged human Hemoglobin subunit beta/ba1 (aa 3-147) recombinant protein
Lane 3 : His-tagged human Hemoglobin subunit delta (aa 3-147) recombinant protein
Lane 4 : His-tagged full length human Hemoglobin subunit delta recombinant protein
Lane 5 : His-tagged human Hemoglobin subunit epsilon (aa 3-147) recombinant protein
Lane 6 : His-tagged human Hemoglobin subunit gamma-2 (aa 2-147) recombinant protein
Lane 7 : His-tagged human Hemoglobin subunit gamma-1 (aa 2-147) recombinant protein
Lane 8 :Recombinant Human Hemoglobin subunit zeta protein (ab95347)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 16 kDaBlocking/Dilutiing buffer and concentration: 5% NFDM/TBST
Exposure time: 100 seconds
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All lanes : Anti-Hemoglobin subunit beta/ba1 + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : Human red blood cell (RBC) lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDaThis data was developed using ab131225, the same antibody clone in a different buffer formulation.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] - BSA and Azide free (ab248384)This data was developed using ab131225, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human endometrium tissue labelling Hemoglobin subunit delta with ab131225 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit delta antibody [EPR8322(B)] - BSA and Azide free (ab248384)This data was developed using ab131225, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling Hemoglobin subunit delta with ab131225 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab248384 has not yet been referenced specifically in any publications.