Recombinant Anti-Hemopexin antibody [EPR5610] (ab124935)
![Western blot - Anti-Hemopexin antibody [EPR5610] (ab124935)](https://www.abcam.com/ps/products/124/ab124935/Images/ab124935-138966-anti-hemopexin-antibody-epr5610-western-blot.jpg "Western blot - Anti-Hemopexin antibody [EPR5610] (ab124935)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5610] to Hemopexin
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Hemopexin antibody [EPR5610]
See all Hemopexin primary antibodies -
Description
Rabbit monoclonal [EPR5610] to Hemopexin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Hemopexin aa 400-500. The exact sequence is proprietary.
-
Positive control
- WB: Human fetal liver and plasma lysates. IHC-P: Human liver tissue. Flow Cyt (intra): HepG2 cells. ICC: HepG2 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 8.30 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5610 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/200.
|
|
| WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 70-75 kDa (predicted molecular weight: 52 kDa).
|
| IP |
1/10 - 1/100.
|
|
| IHC-P | (1) |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| ICC/IF |
1/100 - 1/250.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/200. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 70-75 kDa (predicted molecular weight: 52 kDa). |
|
IP
1/10 - 1/100. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/250. |
Target
-
Function
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Belongs to the hemopexin family.
Contains 5 hemopexin-like domains. -
Post-translational
modificationsN- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 3263 Human
- Omim: 142290 Human
- SwissProt: P02790 Human
- Unigene: 426485 Human
-
Alternative names
- Beta 1B glycoprotein antibody
- Beta-1B-glycoprotein antibody
- FLJ56652 antibody
see all
Images
-
Western blot - Anti-Hemopexin antibody [EPR5610] (ab124935)All lanes : Anti-Hemopexin antibody [EPR5610] (ab124935) at 1/10000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : Human plasma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 70-75 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] (ab124935)](https://www.abcam.com/ps/products/124/ab124935/Images/ab124935-219902-anti-hemopexin-antibody-epr5610-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] (ab124935)This image is courtesy of an Abreview submitted by Steffen Rickeltab124935 staining Hemopexin in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/200 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
-
![Immunocytochemistry/ Immunofluorescence - Anti-Hemopexin antibody [EPR5610] (ab124935)](https://www.abcam.com/ps/products/124/ab124935/Images/ab124935-6-anti-hemopexin-antibody-epr5610-immunocytochemistry-hepg2-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hemopexin antibody [EPR5610] (ab124935)Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) labeling Hemopexin with purified ab124935 at 1/200 dilution (10 µg/ml). Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.8 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody. -
![Flow Cytometry (Intracellular) - Anti-Hemopexin antibody [EPR5610] (ab124935)](https://www.abcam.com/ps/products/124/ab124935/Images/ab124935-287279-anti-hemopexin-antibody-epr5610-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Hemopexin antibody [EPR5610] (ab124935)Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Hemopexin (red) with ab124935 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] (ab124935)](https://www.abcam.com/ps/products/124/ab124935/Images/ab124935-138967-anti-hemopexin-antibody-epr5610-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] (ab124935)ab124935, at 1/250 dilution, staining Hemopexin in paraffin-embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![OI-RD Scanning - Anti-Hemopexin antibody [EPR5610] (ab124935)](https://www.abcam.com/ps/products/124/ab124935/Images/ab124935-138965-anti-hemopexin-antibody-epr5610.jpg)
OI-RD Scanning - Anti-Hemopexin antibody [EPR5610] (ab124935) -
![Anti-Hemopexin antibody [EPR5610] (ab124935)](https://www.abcam.com/ps/products/124/ab124935/Images/ab124935-7-benefits-of-recombinant-antibodies.png)
Anti-Hemopexin antibody [EPR5610] (ab124935)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab124935 has been referenced in 2 publications.
- Canesin G et al. Scavenging of Labile Heme by Hemopexin Is a Key Checkpoint in Cancer Growth and Metastases. Cell Rep 32:108181 (2020). PubMed: 32966797
- Lim JH et al. Novel urinary exosomal biomarkers of acute T cell-mediated rejection in kidney transplant recipients: A cross-sectional study. PLoS One 13:e0204204 (2018). PubMed: 30226858