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RecombinantRabMAb

Recombinant Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)

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Flow Cytometry (Intracellular) - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
  • OI-RD Scanning - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
  • Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5610] to Hemopexin - BSA and Azide free
  • Suitable for: ICC/IF, WB, IP, IHC-P, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 HRP Unconjugated

Overview

  • Product name

    Anti-Hemopexin antibody [EPR5610] - BSA and Azide free
    See all Hemopexin primary antibodies

  • Description

    Rabbit monoclonal [EPR5610] to Hemopexin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IP, IHC-P, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human liver tissue. Flow Cyt (intra): HepG2 cells. ICC/IF: HepG2 cells

  • General notes

    ab226099 is the carrier-free version of ab124935.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab226099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 70-75 kDa (predicted molecular weight: 52 kDa).
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)
Use at an assay dependent concentration.
Notes
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 70-75 kDa (predicted molecular weight: 52 kDa).
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)
Use at an assay dependent concentration.

Target

  • Function

    Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.

  • Tissue specificity

    Expressed by the liver and secreted in plasma.

  • Sequence similarities

    Belongs to the hemopexin family.
    Contains 5 hemopexin-like domains.

  • Post-translational
    modifications

    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Beta 1B glycoprotein antibody
      • Beta-1B-glycoprotein antibody
      • FLJ56652 antibody
      • HEMO antibody
      • HEMO_HUMAN antibody
      • Hemopexin antibody
      • Hpx antibody
      • HX antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
      Flow Cytometry (Intracellular) - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)

      Intracellular Flow Cytometry analysis of HepG2  (Human hepatocellular carcinoma epithelial cell) cells labeling Hemopexin (red) with ab124935 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue). 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124935) 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)This image is courtesy of an Abreview submitted by Steffen Rickelt

      ab124935 staining Hemopexin in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/200 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124935).

    • Immunocytochemistry/ Immunofluorescence - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
      Immunocytochemistry/ Immunofluorescence - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
      This data was developed using the same antibody clone in a different buffer formulation (ab124935).
      Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) labeling Hemopexin with purified ab124935 at 1/200 dilution (10 µg/ml). Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.8 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
      Negative control: PBS instead of the primary antibody.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)

      ab124935, at 1/250 dilution, staining Hemopexin in paraffin-embedded Human liver tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124935).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
      OI-RD Scanning - Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)

      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124935).

    • Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)
      Anti-Hemopexin antibody [EPR5610] - BSA and Azide free (ab226099)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab226099? Please let us know so that we can cite the reference in this datasheet.

    ab226099 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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