Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501)
![Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501)](https://www.abcam.com/ps/products/2/ab2501/Images/ab2501-280731-anti-heparan-sulfate-proteoglycan-2-antibody-a7l6-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501)")
Key features and details
- Rat monoclonal [A7L6] to Heparan Sulfate Proteoglycan 2/Perlecan
- Suitable for: ICC, IHC-P, IP, IHC-Fr, WB
- Reacts with: Mouse, Rat, Cow, Human, Pig, Fish
- Isotype: IgG2a
Overview
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Product name
Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6]
See all Heparan Sulfate Proteoglycan 2/Perlecan primary antibodies -
Description
Rat monoclonal [A7L6] to Heparan Sulfate Proteoglycan 2/Perlecan -
Host species
Rat -
Specificity
Recognizes domain IV of heparan sulphate proteoglycan 2 / perlecan. The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to heparitinase treatment. -
Tested applications
Suitable for: ICC, IHC-P, IP, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human, Pig, Fish -
Immunogen
Tissue, cells or virus corresponding to Heparan Sulfate Proteoglycan 2/Perlecan. High molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.
Database link: P98160 -
General notes
Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (> 400 kD) and one with a small core protein (30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.
Source: A7L6 is a Rat monoclonal IgG2a antibody derived by fusion of X63 Ag8.653 Mouse myeloma cells with spleen cells from a Fisher Rat immunized with high molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.
Formulation: The vial contains 100 ul 1 mg/ml monoclonal purified antibody in PBS containing 0.09% sodium azide.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (> 400 kD) and one with a small core protein (30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors. -
Clonality
Monoclonal -
Clone number
A7L6 -
Myeloma
x63-Ag8.653 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
Use amplification with ABC (avidin biotin complex). |
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| IHC-P | (6) |
Use at an assay dependent concentration.
Use amplification with ABC (avidin biotin complex). |
| IP |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration.
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| Notes |
|---|
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ICC
Use at an assay dependent concentration. Use amplification with ABC (avidin biotin complex). |
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IHC-P
Use at an assay dependent concentration. Use amplification with ABC (avidin biotin complex). |
|
IP
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
Target
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Function
Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.
Endorepellin in an anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.
The LG3 peptide has anti-angiogenic properties that require binding of calcium ions for full activity. -
Tissue specificity
Found in the basement membranes. -
Involvement in disease
Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1) [MIM:255800]; a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses.
Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH) [MIM:224410]. The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage. -
Sequence similarities
Contains 4 EGF-like domains.
Contains 22 Ig-like C2-type (immunoglobulin-like) domains.
Contains 11 laminin EGF-like domains.
Contains 3 laminin G-like domains.
Contains 3 laminin IV type A domains.
Contains 4 LDL-receptor class A domains.
Contains 1 SEA domain. -
Post-translational
modificationsProteolytic processing produces the C-terminal angiogenic peptide, endorepellin. This peptide can be further processed to produce the LG3 peptide.
N- and O-glycosylated; contains three heparan sulfate chains. The LG3 peptide contains at least three and up to five potential O-glycosylation sites but no N-glycosylation. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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Database links
- Entrez Gene: 444872 Cow
- Entrez Gene: 3339 Human
- Entrez Gene: 15530 Mouse
- Entrez Gene: 313641 Rat
- Omim: 142461 Human
- SwissProt: P98160 Human
- SwissProt: Q05793 Mouse
- Unigene: 562227 Human
see all -
Alternative names
- Basement membrane specific heparan sulfate proteoglycan core protein antibody
- Endorepellin (domain V region) antibody
- Heparan sulfate proteoglycan of basement membrane antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501)
IHC image on a frozen section of human colon showing strong reactivity in the extracellular matrix and basement membrane.
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![Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501)](https://www.abcam.com/ps/products/2/ab2501/Images/ab2501-5878-anti-heparan-sulfate-proteoglycan-2-antibody-a7l6-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501)Immunohistochemistry on frozen section of Human kidney showing strong reactivity in the extracellular matrix and basement membrane.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab2501 has been referenced in 24 publications.
- Wang R et al. Mitochondrial transfer from bone-marrow-derived mesenchymal stromal cells to chondrocytes protects against cartilage degenerative mitochondrial dysfunction in rats chondrocytes. Chin Med J (Engl) 134:212-218 (2020). PubMed: 32858593
- Damodarasamy M et al. The microvascular extracellular matrix in brains with Alzheimer's disease neuropathologic change (ADNC) and cerebral amyloid angiopathy (CAA). Fluids Barriers CNS 17:60 (2020). PubMed: 32993718
- Vaisar T et al. Parallel Murine and Human Plaque Proteomics Reveals Pathways of Plaque Rupture. Circ Res 127:997-1022 (2020). PubMed: 32762496
- Templin AT et al. Loss of perlecan heparan sulfate glycosaminoglycans lowers body weight and decreases islet amyloid deposition in human islet amyloid polypeptide transgenic mice. Protein Eng Des Sel 32:95-102 (2019). PubMed: 31769491
- Horsley H et al. A urine-dependent human urothelial organoid offers a potential alternative to rodent models of infection. Sci Rep 8:1238 (2018). PubMed: 29352171