Anti-Hepatitis C Virus E1 antibody (ab110972)
Key features and details
- Goat polyclonal to Hepatitis C Virus E1
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Hepatitis C virus
- Isotype: IgG
Overview
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Product name
Anti-Hepatitis C Virus E1 antibody
See all Hepatitis C Virus E1 primary antibodies -
Description
Goat polyclonal to Hepatitis C Virus E1 -
Host species
Goat -
Tested applications
Suitable for: ELISA, WB, ICC/IFmore details -
Species reactivity
Reacts with: Hepatitis C virus -
Immunogen
Recombinant Hepatitis C Virus E1 (genotype 1a).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Purification notes
ab110972 is >95% pure by Sodium sulfate precipitation and ion exchange chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent dilution. Reacts with genotype 1a.
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| WB |
Use at an assay dependent dilution. Predicted molecular weight: 23 kDa. Reacts with genotypes 1b and 2a.
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| ICC/IF |
Use at an assay dependent dilution. Reacts with genotype 1b, but does not react with genotype 2a.
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| Notes |
|---|
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ELISA
Use at an assay dependent dilution. Reacts with genotype 1a. |
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WB
Use at an assay dependent dilution. Predicted molecular weight: 23 kDa. Reacts with genotypes 1b and 2a. |
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ICC/IF
Use at an assay dependent dilution. Reacts with genotype 1b, but does not react with genotype 2a. |
Target
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Relevance
Envelope glycoproteins E1 and E2 are involved in virus attachment to the host cell as well as in virus endocytosis and fusion with host membrane. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection. -
Cellular localization
Endoplasmic reticulum membrane; Single-pass type I membrane protein -
Database links
- Entrez Gene: 951475 Hepatitis C virus
- SwissProt: Q03463 Hepatitis C virus
- SwissProt: Q9WMX2 Hepatitis C virus
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Alternative names
- E1 protein antibody
- Envelope glycoprotein E1 antibody
- Gp32 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab110972 has not yet been referenced specifically in any publications.