Recombinant Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (ab238974)
![Western blot - Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (ab238974)](https://www.abcam.com/ps/products/238/ab238974/Images/ab238974-317637-238974.jpg "Western blot - Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (ab238974)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18074] to Hepcidin-25 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free
See all Hepcidin-25 primary antibodies -
Description
Rabbit monoclonal [EPR18074] to Hepcidin-25 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human plasma, serum and liver lysate.
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General notes
ab238974 is the carrier-free version of ab187778.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18074 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 9, 3 kDa (predicted molecular weight: 9 kDa).
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| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 9, 3 kDa (predicted molecular weight: 9 kDa). |
Target
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Function
Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005).
Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11113131, PubMed:11034317). -
Tissue specificity
Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine. -
Involvement in disease
Hemochromatosis 2B -
Sequence similarities
Belongs to the hepcidin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 57817 Human
- Omim: 606464 Human
- SwissProt: P81172 Human
- Unigene: 8821 Human
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Form
Hepcidin produces a propeptide of 84 amino acids that undergoes enzymatic cleavage into mature peptides of 20, 22, and 25 amino acids. Active peptides are rich in cysteines that form intramolecular bonds and stabilize the beta-sheet structure -
Alternative names
- Hamp antibody
- HEPC antibody
- HEPC_HUMAN antibody
see all
Images
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Western blot - Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (ab238974)All lanes : Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/1000 dilution
Lane 1 : Human plasma
Lane 2 : Human serum
Lane 3 : Human liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP), preadsorbed at 1/50000 dilution
Predicted band size: 9 kDa
Observed band size: 3 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST.
Dilution buffer: 1% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187778).
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![Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (ab238974)](https://www.abcam.com/ps/products/238/ab238974/Images/ab238974-2-benefits-of-recombinant-antibodies.png)
Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (ab238974)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab238974 has not yet been referenced specifically in any publications.
